1. MBV2010/BIO2140 Colloquium, April 9

2. RULES Multiple choice Only one correct answer 30-60 seconds to answer No textbook, computer, mobile phone, please There will be a prize for the group with the most correct answers.

3. Most small nuclear RNA genes are transcribed by A RNA polymerase I B RNA polymerase II C RNA polymerase III D RNA polymerase IV 1

4. Most small nuclear RNA genes are transcribed by 1

5. The carboxy-terminal domain of RNA polymerase II consists of a repeated A heptapeptide (7 repeats) B hexapeptide (6 repeats) C octapeptide (8 repeats) D decapeptide (10 repeats) 2

6. The carboxy-terminal domain of RNA polymerase II consists of a repeated 2 Tyr-Ser-Pro-Thr-Ser-Pro-Ser

7. The human CTD consists of A 27 repeats B 32 repeats C 45 repeats D 52 repeats 3 Tyr-Ser-Pro-Thr-Ser-Pro-Ser

8. The human CTD consists of 3 Tyr-Ser-Pro-Thr-Ser-Pro-Ser

9. The TATA-binding protein (TBP) is part of A Transcription factor IIA B Transcription factor IIB C Transcription factor IID D Transcription factor IIE 4

10. The TATA-binding protein (TBP) is part of 4

11. FACT is a protein complex involved in A Transcription B Replication C Translation D Recombination 5 ?

12. FACT is a protein complex involved in 5 FACT = facilitates chromatin transcription

13. The rho protein mediates A Initiation of transcription B Initiation of replication C Site-specific recombination D Termination of transcription 6

14. The rho protein mediates 6

15. Which type of intron does not require a branch site for splicing? A Group I introns B GU-AG introns C AU-AC introns D Group II introns 7

16. Which type of intron does not require a branch site for splicing? 7

17. Which of the following proteins plays a role in splice site selection? A snRNPs B SR proteins C Sxl protein D U2 8

18. Which of the following proteins plays a role in splice site selection? 8

19. Amino acids are linked to tRNAs at A the 5’ terminus of tRNAs B the adenine base at the 3’ terminus C the ribose at the 3’ terminus D the phosphate linking the 3’ terminal nucleotides of tRNAs 9

20. Amino acids are linked to tRNAs at 9

21. Which of the following components of the translation machinery has proofreading capability? A ribosomal RNAs B ribosomal proteins C translation initiation factors D tRNA synthetases 10

22. Which of the following components of the translation machinery has proofreading capability? 10

23. Peptide bond formation is catalyzed by A ribosomal RNA B ribosomal proteins C translation factors D all of the above 11

24. Peptide bond formation is catalyzed by 11