1. Drs. Wei Tian & Yanhui Chen Sep-Dec. 2014

2. Main Content Chromatography Analysis Gas Chromatography (GC) High Performance Liquid Chromatography (HPLC) Gel Permeation Chromatography (GPC) Size Exclusion Chromatography/Multiangle Laser Light Scattering (SEC/MALLS) (Key point)

3. Petrochemical Industry Food Analysis Environmental Monitoring Pesticide Residue Function Detect the amount and type of the compounds in the samples Application

4. HPLC is suitable for separation and analysis of high boiling point, poor thermal stability, high polarity, high molecular weight and ionic compounds. As long as the sample can be dissolved in the mobile phase, it is suitable for the detector and can be directly analyzed. Characteristics and Advantages

5. Separation of chromatographic column 1.Molecular weight, molecular weight distribution, and polymer additives 2.Reaction Tracking and Kinetics 3.Composition distribution of the block copolymer Main Application

6. Multiangle Laser Light Scattering Quasielastic Pump Differential Refractometer Viscosity Meter SEC System

7. (1) Polymer samples are first dissolved in appropriate solvent, and then injected into SEC system; the pump can supply the power and drive the solvent flowing in SEC system. (2) After an adequate separation, polymer solution goes into the multiangle laser light scattering, and then into quasielastic, or viscosity meter. Both of them are combined with data wires and differential refractometer to get output signal; (3) The long-range structure information of polymer sample can be obtained by computerized processing. Principle of Work （ Understand ）

8. (1) To determine molecular weight and its distribution; (2) To test parameters in solution, such as molecular rotation radius, end-to-end distance, second virial coefficient; (3) To analyze the size and distribution of polymer aggregates ranging from nanometer to micrometer. Functions （ Master ）

9. (1) The preparation and test conditions of polymer samples; (2) Reasons for the difference between theory and practice molecular weight; (3) Objective analysis to the experimental results. ×√√ Three important problems （ Master key ）

10. Description Macromolecules, 2009, 42(3): 640–651

11. Acknowledgement Wei Tian thanks for the help and source materials from graduate students of Applied Chemistry Department of School of Science.