1. Analyticon Biotechnologies AG
Lichtenfels - Germany

2. Basics of Hematology
Hemolyzer ®

3. Measuring Principles
Laser light scatter technology for 5-part WBC differential
Impedance measurement for WBC, RBC und PLT
Light absorbance for measuring HGB
3-diff (H3) impedance only

4. Types of blood cells RBC: 95% of all blood cells
PLT: ~4.8% of all blood cells
WBC: ~0.2% of all blood cells
NEU: 40-75%
LYM: 20-45%
MON: 2-8%
EOS: 1-6%
BAS: 0-1%
95% RBC
40-45% of Blodd volume = RBC (hematocrit)
Neu ~ 40-75% of WBC
target & destroy bacteria, virusses & fungi
Lym ~ 20-45% of WBC
(B-; T-; & NK-cells) identify & attack „invaders“ & infected/abnormal cells
Mon ~ 2-8% of WBC
Monophages (waste disposal) & dendritic cells (showcasing antigens to activate T-cells)
Eos ~ 1-6% of WBC
target mlticellular parasites
Bas ~ 0-1% of WBC
release histamin, involved in asthma & allergies

5. WBCs selectively shrank to nuclei
Differential Lysing Process
PLT & RBC LYM MON GRAN
RBCs destroyed
WBCs selectively shrank to nuclei

6. 3-part Differential WBC Histogram
PLT & RBC LYM MON GRAN
& HGB

7. 4-part Differential WBC Scattergram
Eosinophiles
Neutrophiles
Basophiles cannot be reliably differentiated from the rest of the white blood cells
Additional optical count is needed
High angle forward scatter
Complexity
Monocytes
Lymphocytes
Size
Low angle forward scatter
RBC GHOST

8. Hemolyzer® 3

9. Hemolyzer® 3
Provides 20 parameters CBC including 3-part WBC differential
3 Histograms: WBC, RBC, PLT
Throughput: 60 samples per hour
6 QC lots, Levey-Jennings diagrams
Database capacity: 10,000
Only 3 reagents (cyanide-free)
Automatic monitoring of reagent status

10. Hemolyzer® 3 Sample Volume: 25 μl (open tube sampling)
Automatic clogging prevention
Large, high resolution colour LCD
Easy, intuitive operation
Interfaces: RS232, USB
Printout via internal thermal printer (optional also via external printer)
Compact design:
350 x 490 x 385 mm
Weight: 15 kg

11. Hemolyzer® 3: Maintenance
Daily Maintenance
Hemolyzer Hypocleaner is inserted as sample and “measured”

12. Hemolyzer® 3: Key Benefits
Safe Operation
The sample is being aspirated inside the analyzer, practically excluding the risk of injuries for the user
High speed
Results within 60 seconds
Reliable Instrument
Easy maintenance by the user

13. Hemolyzer® 3: Competitors
Model
Hemolyzer® 3
Cell-dyn® 1800
AC•T Diff
AC•T Diff2
ABX Micros 60
Distributor
Analyticon
Abbott
Beckman Coulter
Horiba
WBC diff. 3
Number of parameters 20 18
Histograms
WBC, RBC, PLT
Throughput (PT/h) 60 50
60 (open); 55(closed)
Sample volume [μl] 25 30 12 10
Open/Closed sampling
ü/-
ü/ü
STAT Function ü
Number of reagents
Bar code reader
optional
Autoloader (capacity) -
Colour display
Touch Screen
Internal printer
RS-232/USB
 ü/-
Database capacity
10,000
250
1; 78 (with smart card) QC
Levey-Jennings
Levey-Jennings, IQAP
Dimensions
Size (WxDxH) [cm]
35x49x38.5
66x44x52
38x42x39
45x49x40
36x42x32
Weight [kg] 15 55 16 14
Operating temperature
15-35°C
18-30°C
16-34°C

14. Hemolyzer® 3: Competitors
Model
Hemolyzer® 3
M 16M/C/20M/C
M 16S/20S
CA 620
KX 21
Distributor
Analyticon
Medonic
Sysmex
WBC diff. 3
Number of parameters 20
16/20
9/16/20 18
Histograms
WBC, RBC, PLT
Throughput (PT/h) 60 65
Sample volume [μl] 25
100 (open);
250 (closed)
300
125 50
Open/Closed sampling
ü/-
ü/ü (C only)
ü/ü
ü/optional
STAT Function ü
Number of reagents 2
Bar code reader
optional
Autoloader (capacity) -
ü (2x20)
Colour display
Touch Screen
Internal printer
RS-232/USB
 ü/ü
Database capacity
10,000
220 QC
Levey-Jennings
Dimensions
Size (WxDxH) [cm]
35x49x38.5
29x41x46
33x43x46
42x35x46
42x48x36
Weight [kg] 15 22 28
Operating temperature
15-35°C
18-32°C

15. Hemolyzer® 3: Competitors
Model
Hemolyzer® 3
BC 3000+
BC 3200
BC 3600
Distributor
Analyticon
Mindray
WBC diff. 3
Number of parameters 20 19 21
Histograms
WBC, RBC, PLT
Throughput (PT/h) 60
Sample volume [μl] 25 13
Open/Closed sampling
ü/-
-/ü
ü/optional
STAT Function ü
Number of reagents 5
Bar code reader
optional
Autoloader (capacity) -
Colour display
Touch Screen
Internal printer
RS-232/USB
ü/ü
Database capacity
10,000
35000
40000 QC
Levey-Jennings
Dimensions
Size (WxDxH) [cm]
35x49x38.5
39x46x40
39x46x42
45x40x45
Weight [kg] 15 28
Operating temperature
15-35°C
15-30°C

16. Hemolyzer® 3: Competitors
Model
Hemolyzer® 3
Celltac α MEK-6400/6410/6420
Mythic 18
H18 light
Celly Plus
Distributor
Analyticon
Nihon Kohden
Orphee
SFRI
Biocode Hycel
WBC diff. 3
Number of parameters 20 18
Histograms
WBC, RBC, PLT
Throughput (PT/h) 60 63
Sample volume [μl] 25 30
9.8
9.6 10
Open/Closed sampling
ü/-
ü/ü
STAT Function ü
Number of reagents 4
Bar code reader
optional
Autoloader (capacity) -
Colour display
Touch Screen
Internal printer
RS-232/USB
Database capacity
10,000
400
1,500
20,000
1,000 QC
Levey-Jennings
Dimensions
Size (WxDxH) [cm]
35x49x38.5
23x45x38.3
25x34x35
33x43x38
32x35.2x36.8
Weight [kg] 15 9 23
9.5
Operating temperature
15-35°C
18-35°C

17. Hemolyzer® 5

18. Hemolyzer® 5
Provides 24 parameters CBC including 5-part WBC differential
2 Histograms: RBC, PLT
2 Scattergrams: 4-diff WBC, BASO
Throughput: 60 samples per hour
6 QC lots, Levey-Jennings diagrams
Database capacity: 100,000
Only 3 reagents (cyanide-free)
Automatic monitoring of reagent status

19. Hemolyzer® 5 Sample Volume: 100 μl (open/closed, whole blood)
Automatic clogging prevention
Large colour LCD touch screen
Easy, intuitive operation
Autoloader (100 samples) optional
Interfaces: RS232, USB
Printout via external Microsoft®
WindowsTM compatible printers

20. Hemolyzer® 5: Dimensions
Compact design: 410 x 465 x 515 mm
Weight: 35 kg (with autoloader: 49 kg)

21. Hemolyzer® 5: Autoloader
Plug-In; can be added any time
Based on linear racks
Up to 100 samples (10 racks)
Cap recognition and cap piercing
Monovette®, Vacutainer®, Vacuette®
Integrated barcode reader
Integrated sample mixer
Using the same sample needle

22. Hemolyzer® 5: 4-Diff Scattergram
Eosinophiles
Neutrophiles
High angle forward scatter
Complexity
Monocytes
Lymphocytes
Size
Low angle forward scatter
RBC GHOST

23. Hemolyzer® 5: Basophile Scattergram
in the 4-part differential the basophiles cannot be reliably differentiated from the rest of the white blood cells
additional optical count is needed for basophiles
in the WBC/HGB dilution all WBC’s are (over)lysed with the exception of the basophiles
after the impedance count the WBC/HGB dilution is transferred to the optical head for BASO count

24. Hemolyzer® 5: Maintenance
Daily Maintenance
Hemolyzer Hypocleaner is inserted as sample and “measured”
Regular Maintenance (depending on throughput)
Cleaning of measurement head
Cleaning of shear valve

25. Hemolyzer® 5: Key Benefits
Safe Operation
The sample is being aspirated inside the analyzer, practically excluding the risk of injuries for the user
Patented Laser Technology
High speed
Results within 60 seconds
Reliable Instrument
Easy maintenance by the user
Autoloader (100 samples)
Upgrade can be done at any time

26. Hemolyzer® 5: Competitors
Model
Hemolyzer® 5
Cell-dyn® 3200 CS
Cell-dyn® 3200 AL
Cell-dyn® 3700 CS
Cell-dyn® 3700 SL
Distributor
Analyticon
Abbott
WBC diff. 5
Number of parameters 24 22 23
Histograms
RBC, PLT
Scattergrams
4diff (L-M-N-E), BAS
WBC, RBC, PLT, RET
WBC, RBC, PLT,
Throughput (PT/h) 60 71 72 90
Sample volume [μl]
100
240 (closed); 150 (open)
240 (closed); 130 (open)
355 (AL); 240 (closed); 130 (open)
Open/Closed sampling
/
STAT Function 
Number of reagents 3 4
Bar code reader
optional
Autoloader (capacity)
optional (100) -
 (50)
 (100)
Standalone
Touch Screen
Internal printer
RS-232/USB
1/4
/-
Database capacity
100,000
10,000 QC
Levey-Jennings
Dimensions
Size (WxDxH) [cm]
40x45x50
83x48x59
83x48x74
76x61x56
76x68x79
Weight [kg] 35 97
106 86
131

27. Hemolyzer® 5: Competitors
Model
Hemolyzer® 5
AC•TTM 5 Diff OV/CP
AC•TTM 5 Diff AL
HmX
ABX Pentra (XL) 80
Distributor
Analyticon
Beckman Coulter
Horiba
WBC diff. 5
Number of parameters 24 26
Histograms
RBC, PLT
WBC, RBC, PLT
Scattergrams
4diff (L-M-N-E), BAS
4diff (L-M-N-E) 
Throughput (PT/h) 60 80 75
Sample volume [μl]
100
53 (CBC + diff); 30 (CBC)
185 (closed); 125 (open)
Open/Closed sampling
/
/ (CP)
STAT Function
Number of reagents 3
Bar code reader
optional
Autoloader (capacity)
optional (100) -
 (100)
Standalone
 (OV)/-(CP)
Touch Screen
Internal printer
 (laser)
RS-232/USB
1/4
/2
Database capacity
100,000
10,000
5,000 QC
Levey-Jennings
IQAP
Dimensions
Size (WxDxH) [cm]
40x45x50
44.4x58x50.1
81.5x58x54
61.5x61.7x84.8
82x57x54cm
Weight [kg] 35
36.2 55
94.5

28. Hemolyzer® 5: Competitors
Model
Hemolyzer® 5
BC 5300
BC 5380
BC 5500
BC 5800
Distributor
Analyticon
Mindray
WBC diff. 5
Number of parameters 24 27 29
Histograms
RBC, PLT 3
Scattergrams
4diff (L-M-N-E), BAS 1
Throughput (PT/h) 60
80 (AL); 60 (manual) 90
Sample volume [μl]
100
20 (CBC + diff); 15 (CBC)
180 (closed); 120 (open)
Open/Closed sampling
/
/-
STAT Function 
Number of reagents 6
Bar code reader
optional
Autoloader (capacity)
optional (100) -
 (30)
optional (50)
Standalone
Touch Screen
Internal printer
RS-232/USB
1/4
/4
/5
Database capacity
100,000
40,000 QC
Levey-Jennings
Dimensions
Size (WxDxH) [cm]
40x45x50
41x53x47
66.4x61.3x58.5
66.4x61.3x58.6
Weight [kg] 35 45 73 76

29. Hemolyzer® 5: Competitors
Model
Hemolyzer® 5
XS 800i
XS 1000i
XT 1800i/2000i
Distributor
Analyticon
Sysmex
WBC diff. 5
Number of parameters 24
24/31
Histograms
RBC, PLT
Scattergrams
4diff (L-M-N-E), BAS 
4diff, WBC/Baso, PLT, Ret (2000i)
Throughput (PT/h) 60
60; AL: 52 80
Sample volume [μl]
100 20
85; 150 (AL)
Open/Closed sampling
/
/-
STAT Function
Number of reagents 3 4
Bar code reader
optional
Autoloader (capacity)
optional (100) -
optional (20)
Standalone
Touch Screen
Internal printer
RS-232/USB
1/4
Database capacity
100,000
10,000 QC
Levey-Jennings
Dimensions
Size (WxDxH) [cm]
40x45x50
32x50x41
32x40x41
53x63x50
Weight [kg] 35 52

30. Hemolyzer® 5: Competitors
Model
Hemolyzer® 5
Celltac E MEK-7222
Celltac E MEK-8222
Mythic 22 OT/CT
Mythic 22 AL
Distributor
Analyticon
Nihon Kohden
Orphee
WBC diff. 5
Number of parameters 24 22
Histograms
RBC, PLT 
Scattergrams
4diff (L-M-N-E), BAS
5-diff
Throughput (PT/h) 60 51 80
50(OT)/45(CT)
Sample volume [μl]
100
55 (CBC + diff); 30(CBC)
56 (CBC + diff); 30 (CBC)
17 (OT)/20 (CT) 20
Open/Closed sampling
/
/optional
/ (CT only)
STAT Function
Number of reagents 3
Bar code reader
optional
Autoloader (capacity)
optional (100) -
 (50)
Standalone
Touch Screen
Internal printer
RS-232/USB
1/4
1/2
1/5
Database capacity
100,000
1,500 QC
Levey-Jennings
Dimensions
Size (WxDxH) [cm]
40x45x50
61.3x58.3x55
25x34x35
48x40x40
38.2x48.5x52
Weight [kg] 35 55 11 25 38