1. Stephen L. Doggett Senior Hospital Scientist Department of Medical Entomology Institute for Clinical Pathology & Medical Research, Westmead Hospital, Westmead NSW 2145

2. Ticks – introduction Ticks – introduction Biology/ecology/lifecycle Biology/ecology/lifecycle Management Management Avoidance Avoidance Overview

3. Worldwide – about 800 sp. Worldwide – about 800 sp.  Australia – 75 (~15 attack humans)  2 groups – Soft & Hard ticks Most important - Ixodes holocyclus Most important - Ixodes holocyclus  Paralysis tick, scrub, bush, shell back  Seed, grass (larval stage) Ticks

4. 1988-2013: 671 specimens 1988-2013: 671 specimens Amblyomma: 3.7% Amblyomma: 3.7% Aponomma: 0.3% Aponomma: 0.3% Haemaphysalis: 1.8% Haemaphysalis: 1.8% Rhipicephalus: 1.3% Rhipicephalus: 1.3% Ixodes: 93% Ixodes: 93%  Ixodes holocyclus: (98%)  Adults: 37%, Nymph: 26%, Larvae: 37% Tick Samples to DME

8. 1mm LarvaNymph Adult Male Adult Female

9. Tick Life Cycle Paralysis tick, Ixodes holocyclus

16. 3m 0.5m Host Seeking Behaviour

17. Most of the year Most of the year Survival dependant on humidity Survival dependant on humidity Most active: Most active:  Following rain  Periods of high humidity Larvae far more common than other stages Larvae far more common than other stages  High mortality in larval stage to nymph When are ticks active?

18. Animal Hosts Red-necked Wallaby House Mouse Magpie Australian Raven Domestic Fowl Human Dog Cat Pig Eastern Grey Kangaroo Norwegian Rat Bush Rat Grasslands Melomys Water Rat Crimson Rosella Pied Butcherbird Echidna Common Dunnart Feathertail Glider Red-legged Pademelon Northern Brown Bandicoot Southern Brown Bandicoot Long-nosed Bandicoot Mountain Brushtail Possum Common Brushtail Possum Brush-tailed Phascogale Koala Swamp Wallaby Rabbit Black Rat After Roberts (1970)

20. Main Host Bandicoot

24. MAKE SURE YOU REMOVE THE HEAD!!! Tick Removal

30. Cultural Control: Behaviour modification Cultural Control: Behaviour modification Physical Control: Exclusion fencing Physical Control: Exclusion fencing Biological Control Biological Control  Parasites/pathogens/predators  Host removal  Habitat modification Chemical Control Chemical Control  Repellents  Habitat treatment  Host treatment Tick Management

31. Tick Control Methods

32. Avoid ticky habitat Avoid ticky habitat Avoid good ticky times Avoid good ticky times  After rain, high humidity Wear light coloured clothing Wear light coloured clothing Check oneself regularly during & after Check oneself regularly during & after Tuck pants into socks, shirts into pants Tuck pants into socks, shirts into pants Remove clothing, place into hot dryer Remove clothing, place into hot dryer Use repellents/permethrin on clothing Use repellents/permethrin on clothing Cultural Control

34. Formulations

35. Gimmicks

36. Australian Pesticides & Veterinary Medicines Authority (National Registration Authority: NRA) Australian Pesticides & Veterinary Medicines Authority (National Registration Authority: NRA) For insecticides/repellents, assess: For insecticides/repellents, assess:  Human Toxicity  Product Efficacy  Environmental Impacts APVMA

37. Active Active Approved use Approved use Registered? Registered? Directions Directions

38. DEET (N,N-Diethyl-meta-toluamide) DEET (N,N-Diethyl-meta-toluamide) Picaridin Picaridin Lemon Eucalyptus Oil (PMD) Lemon Eucalyptus Oil (PMD) IR3535 IR3535 Citronella, melaleuca oil Citronella, melaleuca oil Repellent Actives “Natural” “Organic” “Safe” & “Effective” =

39. Active% Protection Time Repellent Efficacy Mosquitoes Mosquitoes  Fradin & Day, NEJM (2002) Active% Protection Time DEET24%302mActive% DEET24%302m DEET20%234mActive% DEET24%302m DEET20%234m DEET6.7%112mActive% DEET24%302m DEET20%234m DEET6.7%112m Citronella10%20mActive% DEET24%302m DEET20%234m DEET6.7%112m Citronella10%20m Citronella5%14mActive% DEET24%302m DEET20%234m DEET6.7%112m Citronella10%20m Citronella5%14m Citronella (WB) 25%0.2m

40. Permethrin = toxicant not a repellent Permethrin = toxicant not a repellent Two forms: Two forms:  DIY, ‘dip’ clothing  Impregnated fabrics (IF) Studies show: Studies show:  IF better protection than DIY  IF more washes than DIY  IF less environmental impacts  Permethin better protection than repellents  Widely used by armies Permethrin Impregnated Clothing

41. Permethrin Impregnated Clothing?

42. Human Safety Risk analysis Risk analysis  What is worse: bites or repellents? DEET first registered, 1957 DEET first registered, 1957  Billions of uses  Recommended by WHO & CDC  Only product recommended by CDC  US EPA: adverse reactions 1:100million EPA: “permethrin factory-treated clothing is unlikely to pose any significant acute or chronic hazard to people” EPA: “permethrin factory-treated clothing is unlikely to pose any significant acute or chronic hazard to people” Read & heed the label!

43. Host Exclusion Fencing

45. Reduce overhanging foliage Reduce overhanging foliage Clearing brush/bushes Clearing brush/bushes Removal of leaf litter/mulch Removal of leaf litter/mulch Maintain lawns <15cm Maintain lawns <15cm Cementing Cementing Inc. sunlight to ground, dec. humidity Inc. sunlight to ground, dec. humidity Achieves most prolonged level of control Achieves most prolonged level of control Habitat Modification

47. Advantages Advantages  Rapid control, 97% within 24 hours  Cost effective  Less environmental damage Disadvantages Disadvantages  Not tick specific  Relatively short term Pyrethroids: Permethrin, Betacyfluthrin, Bifenthrin Pyrethroids: Permethrin, Betacyfluthrin, Bifenthrin Chemical Control

48. Pyrethroid Pyrethroid Toxic to lower vertebrates Toxic to lower vertebrates Strongly binds to the soil Strongly binds to the soil  reduced contamination risk Recommended by WHO for mosquito c. Recommended by WHO for mosquito c. BUT DOES IT WORK? BUT DOES IT WORK? Two formulations: Two formulations:  Emulsifiable Concentration, Granular Bifenthrin

49. Bifenthrin - Formulations Granular (Bithor, Brigade, Vigilanti) Can be used against all stages Directions for use: “Applications should be made in the spring to control larvae and nymphs that reside in the soil and leaf litter…” Application: Hand, fertilizer spreaders, backpack, aerial

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51. www.medent.usyd.edu.au