1. VectorBase Seth Redmond Imperial College, London seth.redmond@imperial.ac.uk

2. VectorBase “A Relational Bioinformatics Resource Center for Arthropod Vectors of Human pathogens” Funded by NIAID (National Institute of Allergy & Infectious Diseases - US) One of a set of BRC’s funded to support large-scale sequencing projects

3. VectorBase Aedes aegyptii (Yellow Fever) A.gambiae (PEST) (Malaria) A.gambiae (M)A.gambiae (S) Culex pipens (Filariasis) Pediculus humanus (Misc - body louse) Ixodes Scapularis (Lyme Disease) Lutzomyia longipalpis (Leishmaniasis) Glossina Morsitans (Sleeping sickness) Rhodnius prolixus (Chagas Disease) Phlebotomus papatasi (Leishmaniasis)

4. VectorBase PROBE ALIGNS COMP ANNOT MAN ANNOT EST ANNOT MAN ANNOT PROT ANNOT HOSTING & INTEGRATION

5. VectorBase

7. CEGG APOLLO DAS server DAS server DAS server DAS server DAS server DAS client

8. VectorBase APOLLO CEGG

9. VectorBase VB DAS use e! DAS client Permanent DAS servers: Rapidly changing features –e.g. manual annotation Low priority / low usage features –e.g. AnoEST clusters ‘Temporary’ servers Preliminary data –e.g. array mappings between genebuilds Collaborative use

10. VectorBase Permanent Servers A Gambiae –Manual annotation VectorBase manual CEGG Immunity set –Comp annotation AnoEST Clusters ReAnoCDS –Transposons A.Aegypti –Comp Annotation AedEST Clusters

11. VectorBase APOLLO CEGG

12. VectorBase APOLLO CEGG

13. VectorBase APOLLO CEGG DAS server DAS server DAS client DAS server

14. VectorBase DAS requests… Versioning –Old features / New assemblies Nested Features –Sim to GFF3, refer to parent ID to allow construction of gene models

15. VectorBase Imperial –Fotis Kafatos –George Christophides –Bob Maccallum EBI –Ewan Birney –Dan Lawson –Martin Hammond –Karyn Megy Harvard –William Gelbart –Susan Russo –Kathy Campbell Notre Dame –Frank Collins –Rob Bruggner –EO. Stinson –Neil Lobo IMBB –Kitsos Louis –Pantelis Topalis