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Yin-Yang Column for Global Protein Identification Column Technology Inc.

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Presentation on theme: "Yin-Yang Column for Global Protein Identification Column Technology Inc."— Presentation transcript:

1 Yin-Yang Column for Global Protein Identification Column Technology Inc.

2 Yin-Yang MDLC-MS/MS The Yin-Yang column combines anion and cation exchange chromatography For the protein identification through multi-LC/MS/MS:

3 Advantages of Yin-yang MDLC-MS/MS ► Three-dimensional LC-MS/MS ► SCX and ANX are complimentary methods for peptide fractionation ► Most complete coverage of peptides and proteins ► Enrichment and high-sensitivity for phosphopeptides ► Identify phospho and non-phospho peptides in the same run.

4 Theoretical pI distribution of identified non-phosphrylated peptides Complementary peptide identification of SCX and SAX

5 9094 3047 10254 SAXSCX 108413 Unique peptides 6184 Proteins SAX SCX 2040 4072 4152 only 1mg protein digests of mouse liver, 5% FPR Another More than 1000 phosphopeptides identified

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