1. How to Write a Scientific Paper
Barbara Gastel, MD, MPH
Professor, Texas A&M University
Knowledge Community Editor, AuthorAID

2. Overview Structure of a scientific paper
Methods/Results/Tables and Figures
Discussion/Introduction/References
Title
Abstract

3. Structure of a Scientific Paper

4. The IMRAD Format for Scientific Papers
Introduction: What was the question?
Methods: How did you try to answer it?
Results: What did you find?
And
Discussion: What does it mean?

5. A More Complete View (Title) (Authors) (Abstract) Introduction Methods
Results
Discussion
(Acknowledgments)
(References)

6. Some Other Structures
IRDAM (Introduction, Results, Discussion, and Methods)
IMRDRD…(Introduction, Methods, a Result, Some Discussion, Another Result, More Discussion )
Other
Question: In your field, what is the usual structure of papers reporting research?

7. Orders of Reading and Writing Sections of a Paper
People read the sections of scientific papers in various orders.
You can write the sections of a scientific paper in any order.
A convenient order in which to write the sections: Methods, Results, Discussion, Introduction

8. Methods, Results, and Tables and Figures

9. Purposes of the Methods Section
To allow others to replicate what you did
In order to test it
In order to do further research
To allow others to evaluate what you did
To determine whether the conclusions seem valid
To determine whether the findings seem applicable to other situations of interest

10. Methods: Basic Information to Include
In most cases, overview of study design
Identification of (if applicable)
Equipment, organisms, reagents, etc used (and sources thereof)
Approval of human or animal research by an appropriate committee
Statistical methods

11. Methods: Amount of Detail to Use
For well-known methods: name of method, citation of reference
For methods previously described but not well known: brief description of method, citation of reference
For methods that you yourself devise: relatively detailed description

12. Methods: The Words and More
Should be written in past tense
In some journals, may include subheads (which can be helpful to readers)
May include tables and figures—for example:
Flowcharts
Diagrams of apparatus
Tables of experimental conditions

13. The Results Section The core of the paper
Often includes tables, figures, or both
Should summarize findings rather than providing data in great detail
Should present results but not comment on them
(Note: Some journals combine the Results and the Discussion.)

14. Verb Tense for the Results Section: Past Tense
Examples:
A total of 417 of the customers replied.
_____ increased, but _____ decreased.
The average temperature was _____.
Three of the dogs died.
This difference was not statistically significant.

15. Results Sections of Papers with Tables or Figures
How much should the information in the text overlap that in the tables and figures?
Not extensive overlap
In general, only the main points from the tables and figures
Perhaps a few of the most important data
Remember to mention every table and figure. Do so as soon as readers might want to see the table or figure.

16. Mentioning Tables and Figures: Some Writing Advice
In citing tables and figures, emphasize the finding, not the table or figure.
Not so good: Table 3 shows that researchers who took the course published twice as many papers per year.
Better: Researchers who took the course published twice as many papers per year (Table 3).

17. Tables: A Few Suggestions
Use tables only if text will not suffice.
Design tables to be understandable without the text.
If a paper includes a series of tables, use the same format for each.
Be sure to follow the instructions to authors.

18. Figures: A Few Suggestions
Use figures (graphs, diagrams, maps, photographs, etc) only if they will help convey your information.
Avoid including too much information in one figure.
Make sure any lettering will be large enough once published.
Follow the journal’s instructions.

19. Discussion Question
If you have data that could be presented in either a table or a figure, how do you decide which one to use?

20. Sources of Further Information
“Almost Everything You Wanted to Know About Making Tables and Figures,” Department of Biology, Bates College, (
Writing and Publishing Scientific Papers, Part 2 (from China Medical Board course), accessible at

21. Discussion, Introduction, and References

22. Discussion
One of the more difficult parts to write, because have more choice of what to say
Often should begin with a brief summary of the main findings
Should answer the question(s) stated in the introduction

23. The Discussion: Some Possible Content
Limitations of the study
For example: small sample size, short follow-up, incomplete data, possible sources of bias, problems with experimental procedures
Better to mention limitations yourself than for peer reviewers and readers to think that you’re unaware of them
If the limitations seem unlikely to affect the conclusions, can explain why

24. The Discussion: Possible Content (cont)
Relationship to findings of other research—for example:
Similarities to previous findings (your own, others’, or both)
Differences from previous findings
Possible reasons for similarities and differences

25. The Discussion: Possible Content (cont)
Applications and implications—for example:
Possible uses of the findings (in agriculture, medicine, industry, public policy, etc)
Relationship of the findings to theories or models:
Do the findings support them?
Do they refute them?
Do they suggest modifications?

26. The Discussion: Possible Content (cont)
Other research needed—for example:
To address questions still unanswered
To address new questions raised by the findings
Other

27. Discussion (cont)
Typically should move from specific to general (opposite of introduction)
In some journals, is followed by a Conclusions section
In some short papers, is called “Comment” rather than “Discussion”

28. Purposes of the Introduction
To provide background
In order to help readers understand the paper
In order to help readers appreciate the importance of the research
To identify the question(s) the research addressed
Sometimes stated as a hypothesis or hypotheses

29. Length of Introduction
Articles in biomedical journals: tend to have short Introductions (a few paragraphs or less)
Articles in some other journals: tend to have long Introductions
What about Introductions in your field?

30. Gearing the Introduction to the Audience
Papers in relatively general journals: Introduction must provide basic background information.
Papers in specialized journals in your field: Introduction can assume that readers have more knowledge about the field.

31. Structure of the Introduction
Introduction typically should be funnel-shaped, moving from general to specific
A common structure:
Information on importance of topic
Highlights of relevant previous research
Identification of unanswered question(s)
Approach you used to seek the answer(s)
(In some fields) your main findings

32. Functions of References
To give credit to others for their work
To add credibility to your work by showing that you used valid information sources
To help show how your work is related to previous work
To help readers find further information

33. References: Importance of Accuracy
Studies show that many references are inaccurate.
For references to fulfill their functions, they must be accurate. Therefore
Make sure that you accurately state what the cited material says.
Make sure that all information in the citation (for example, author list, article title, journal title, volume, year, pages) is accurate.

34. Another Reason Your References Should Be Accurate
Often, authors whose work you cite will be your peer reviewers. Inaccurate references to their work will not impress them favorably.

35. Formats Various formats exist for citation in text—for example:
Accuracy of references is important (Day and Gastel, 2006).
Accuracy of references is important.3
Various formats exist for items in reference lists—for example:
Pineda D Communication of science in Colombia. Sci. Ed. 26:91-92.
Pineda D. Communication of science in Colombia. Sci Ed 2003;26:91-2.

36. A Reminder Be sure to use the format used by your target journal.
For the citations in the text
For the reference list

37. Citation Management Software
Examples: EndNote, Reference Manager
Allow you to keep a database of references
Provide the citations and references in the proper format for your target journal

38. Placement of Citations
Ambiguous:
This disease has been reported in humans, dogs, rabbits, and squirrels (Tuda and Gastel, 1997; Xie and Lozano, 2008; Flores, 2002).
This disease has been reported in humans, dogs, rabbits, and squirrels.1,4,7
Clear:
This disease has been reported in humans (Tuda and Gastel, 1997), dogs (Xie and Lozano, 2008), and rabbits and squirrels (Flores, 2002).
This disease has been reported in humans,1 dogs,4 rabbits,7 and squirrels.7

39. Other Advice on References
Cite only items that you have read.
Check each reference against the original source.
Carefully follow the journal’s instructions to authors.
Use other articles in the same journal as models.

40. Title

41. Title
The fewest possible words that adequately indicate the contents of the paper
Important in literature searching
Should not include extra words, such as “a study of”
Should be specific enough but not overly narrow

42. Abstract

43. The Abstract Briefly summarizes the paper
Should be organized like a scientific paper (in sort of a mini-IMRAD format)
In some fields, there are structured abstracts (with standardized headings).

44. Example of a Short Abstract
From the following short paper: Pitkin RM, Burmeister LF. Prodding tardy reviewers: a randomized comparison of telephone, fax, and . JAMA 2002;287:

45. Context To compare telephone, fax, and methods of prodding tardy reviewers. Methods Randomized trial conducted January 1998 through June 1999 at the main editorial office of Obstetrics & Gynecology. Reviewers who had failed to file reviews by 28 days after being sent manuscripts (7 days after deadline) were sent identical messages in oral (telephone) or written (fax and ) form inquiring as to the status of review, asking for its completion as soon as possible, and requesting it be sent by fax or . Results Of 378 reviewers, proportions returning reviews within 7 days were essentially identical: telephone, 85 (68%) of 125; fax, 86 (67%) of 129; and , 84 (67%) of 124 (P=.59). In the two thirds who responded, the mean time to return reviews did not differ among the 3 groups. Conclusion Contacting tardy reviewers resulted in a review being received within 7 days in about two thirds of cases, and it made no difference if the contact was made by telephone, fax, or .

46. Example of an Abstract from Water Research
From: Skraber S, Ogorzaly L, Helmi K, Maul A, Hoffmann L, Cauchie H-M, Gantzer C. Occurrence and persistence of enteroviruses, noroviruses and F-specific RNA phages in natural wastewater biofilms. Water Research 2009;43(19):

47. Enteroviruses and noroviruses are pathogenic viruses excreted by infected individuals. Discharged in wastewaters, some of these viruses can be captured by biofilms. In the present study, we assessed the occurrence and persistence of these viruses in wastewaters and in corresponding biofilms. Natural wastewaters and biofilms were analyzed monthly from January to July using real-time RT-PCR. Enterovirus RNA was detected in wastewater in June while norovirus RNA was detected from January to March. In contrast, biofilm analysis revealed the presence of both enterovirus and norovirus genomes throughout the study period. For instance, enterovirus and norovirus genogroups (GG) I and II were detected in 50, 46 and 37% of the biofilm samples, respectively (n = 24). In a laboratory

48. experiment, persistence of norovirus GGI RNA (quantified using molecular techniques) and F-specific bacteriophages (quantified using both culture and molecular techniques) was assessed in wastewater and corresponding naturally-contaminated biofilms at both 4 and 20 °C. The concentrations of viral genomes (norovirus GGI and F-specific RNA phage) were very stable in biofilms. Indeed, no significant decrease was observed during the persistence experiment that lasted 49 days. Furthermore, regardless of our experimental conditions, viral genome and infectious F-specific bacteriophages persisted longer in biofilm than in wastewater. According to our results, wastewater biofilms may contribute to the persistence and dispersal of pathogenic viruses outside of epidemic periods.

49. Thank you!