1. Biological Membranes Penghui Lin Nov. 15. 2013

2. Membranes http://www.uvm.edu/~inquiryb/webquest/fa06/mvogenbe/

3. Diffusion across plasma membrane http://en.wikipedia.org/wiki/Cell_membrane

4. Plasma Membrane Pabst G. Applications of neutron and X-ray scattering to the study of biologically relevant model membranes J.Chem Phys Lipids. 2010 Jun;163(6):460-79J

5. Man-made membrane http://bioweb.wku.edu/courses/biol115/wyatt/biochem/lipid/Lipid_2.asp

6. Liquid crystals Nematic phase Smectic phases Chiral phases http://en.wikipedia.org/wiki/Liquid_crystal

7. Sample preparation Jörg Fitter, Thomas Gutberlet, John Katsaras. Neutron Scattering in Biology, Techniques and Applications. 2006 ISBN: 978-3-540-29108-4 (Print) 978-3-540-29111-4 (Online)

8. Incident angle = reflected angle Specular Neutron Reflectivity Jörg Fitter, Thomas Gutberlet, John Katsaras. Neutron Scattering in Biology, Techniques and Applications. 2006 ISBN: 978-3-540-29108-4 (Print) 978-3-540-29111-4 (Online)

9. Density profile ρ 0 (z) Fourier Synthesis Jörg Fitter, Thomas Gutberlet, John Katsaras. Neutron Scattering in Biology, Techniques and Applications. 2006 ISBN: 978-3-540-29108-4 (Print) 978-3-540-29111-4 (Online)

10. Lipid bilayer phases

11. Location of Cholesterol Pabst G. Applications of neutron and X-ray scattering to the study of biologically relevant model membranes J.Chem Phys Lipids. 2010 Jun;163(6):460-79J

12. Location of cholesterol Pabst G. Applications of neutron and X-ray scattering to the study of biologically relevant model membranes J.Chem Phys Lipids. 2010 Jun;163(6):460-79J

13. Forces between membranes Disjoining pressureOsmotic pressure Pabst G. Applications of neutron and X-ray scattering to the study of biologically relevant model membranes J.Chem Phys Lipids. 2010 Jun;163(6):460-79J

14. Osmotic stress experiment Neutron/ Pabst G. Applications of neutron and X-ray scattering to the study of biologically relevant model membranes J.Chem Phys Lipids. 2010 Jun;163(6):460-79J

15. Thermal unbinding Pabst G. Applications of neutron and X-ray scattering to the study of biologically relevant model membranes J.Chem Phys Lipids. 2010 Jun;163(6):460-79J

16. Single component membrane Pabst G. Applications of neutron and X-ray scattering to the study of biologically relevant model membranes J.Chem Phys Lipids. 2010 Jun;163(6):460-79J

17. Lipid mixtures and domains Ideal mixing: no phase separation Non-ideal or non-random mixing: compositional fluctuation happens while no stable domains formed Macroscopic phase separation: micron-size stable lipid domains are formed

18. No phase separation Membrane thickness Fluctuation parameter in DMPC bilayers at 35° Pabst G. Applications of neutron and X-ray scattering to the study of biologically relevant model membranes J.Chem Phys Lipids. 2010 Jun;163(6):460-79J

19. Macroscopic phase separation DPPC/DOPC/cholesterol Pabst G. Applications of neutron and X-ray scattering to the study of biologically relevant model membranes J.Chem Phys Lipids. 2010 Jun;163(6):460-79J

20. Supramolecular properties Diffused scattering Tilted phase Interdigitated phase Pabst G. Applications of neutron and X-ray scattering to the study of biologically relevant model membranes J.Chem Phys Lipids. 2010 Jun;163(6):460-79J

21. Bicelle mixtures Pabst G. Applications of neutron and X-ray scattering to the study of biologically relevant model membranes J.Chem Phys Lipids. 2010 Jun;163(6):460-79J

22. Bicelle mixtures Pabst G. Applications of neutron and X-ray scattering to the study of biologically relevant model membranes J.Chem Phys Lipids. 2010 Jun;163(6):460-79J

23. Fluctuation change upon antimicrobial peptide binding Jörg Fitter, Thomas Gutberlet, John Katsaras. Neutron Scattering in Biology, Techniques and Applications. 2006 ISBN: 978-3-540-29108-4 (Print) 978-3-540-29111-4 (Online)

24. Membrane rigidity change Pabst G. Applications of neutron and X-ray scattering to the study of biologically relevant model membranes J.Chem Phys Lipids. 2010 Jun;163(6):460-79J

25. Pabst G. Applications of neutron and X-ray scattering to the study of biologically relevant model membranes J.Chem Phys Lipids. 2010 Jun;163(6):460-79J

26. Thanks! Jörg Fitter, Thomas Gutberlet, John Katsaras. Neutron Scattering in Biology, Techniques and Applications. 2006 ISBN: 978-3-540-29108-4 (Print) 978-3-540-29111-4 (Online)