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Published byAlvin Wilkinson Modified over 9 years ago
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Basics of Chromatography Separation of Analytes Type of Equilibrium
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Basics of Chromatography Ability to dissolve in mobile phase Interaction with stationary phase Time on X axis Response on Y
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Chromatography Types
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Separation dependent on time on the column (along with other factors)
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Retention time of the analyte Retention time of the Mobile Phase
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Basics of Chromatography Average linear flow rate Average linear flow of mobile phase
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Plate Height Theoretical Measure of Efficiency
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Fig. 26-6 Determining standard deviation from a peak Std dev = τW = 4 τ
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Basics of Chromatography Capacity Factor Selectivity Factor
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Basics of Chromatography The Van Deemter Equation H = A + B/u + Cu
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Multiple Flow Paths Tortuosity
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Components of the VanDeemter
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Van Deemter Plot
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How Plate Height is effected by particle size in solid packing
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Basics of Chromatography Column Efficiency
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Resolution
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Basics of Chromatography Resolution
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The Effect of Mobile Phase on Retention
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More on Resolution
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