1. Progress Report on Miniaturized Liquid Chromatography for NeSSI Scott Gilbert, CPAC Visiting Scholar Crystal Vision Microsystems LLC Atofluidic Technologies, LLC

2. Lab-on-chip approach Simplicity of operation Reduction of moving parts – no (mechanical) pumps Robustness Inexpensive and facile maintenance

3. Chip Layout 20mm Column Inlet lines Detector electrodes

4. From Old Setup…

5. …To New NeSSI Mountable Design

6. Split flow approach to sampling Liters per minute microliters per minute nanoliters per minute

7. Fluid Flow Strategy

8. Functional Integration of  -fluidic LC Chip for On-line Sample Pretreatment micromixer diluent in column mobile phase in sample in

9. Rapid Prototyping Using Quick Photolithographic Method Inkjet printed photomaskFinished product

10. Front Mounted Microfluidic Chip

11. Back-Mounted Valves

12. Quantitation of flow splitting