1. HANDS-ON APPROACH TO CHROMATOGRAPHY Nataša Gros 1 Margareta Vrtačnik 2, M. Filomena Camões 3 1 Faculty of Chemistry and Chemical Technology, 2 Faculty of Natural Sciences and Engineering, University of Ljubljana, Slovenia 3 University of Lisbon, Portugal Project No: SI/03/B/F/PP-176012 © FKKT, Ljubljana, 2003-2005

2. Navigation – next Navigation – next Navigation – back to modules Navigation – back to modules Experiment Video clip Video clip LEGEND Next slide

3. 1. Introduction to chromatographyIntroduction to chromatography 2. Gas chromatographyGas chromatography 3. Liquid chromatographyLiquid chromatography 4. Chromatography –fields of applicationChromatography –fields of application

4. Examples of slides

5. A simplified gas chromatograph Cylinder with carrier gas Flow regulator Injector Column with stationary phase Detector display  Record of measurement values

6. Chromatogram Graphic presentation of measurement values vs time Chromatographic peak of the component eluted first Chromatographic peak of the component eluted second RESPONSERESPONSE

7. A simplified liquid chromatograph

8. Chromatographic system with vacuum water-jet pump Container with eluent Pump Sample injection port Column with stationary phase Detector display  Record of measurement values

9. “ Separating the dyes from mint aroma Video: click on the icon

10. “ Separation of the dyes from mint aroma Green component blue component

11. Acknowledgements This Project has been carried out with the support of the European Community. The content of this project does not necessarily reflect the position of the European Community, or National Agency. For this reason neither the National Agency nor the European Community take any responsibility regarding the content of the report.