1. Report 0218-2008: Steryl Glucoside Calibration By Haiying Tang

2. Steryl glucoside (SG) ISTD1 ISTD2 GC-FID Chromatography: Steryl glucoside (Standard,Matreya)

3. GC-FID Chromatography: Sterol glycoside (Standard,Matreya) ISTD2 SG

4. GC-FID Chromatography STD:Mono1,3,5 STD:SG STD: Total glycerol glycerol ISTD1 ISTD2 Diglyceridestriglycerides ISTD1 Mono1 Mono Mono3,5 SG

5. Calibration Curve

6. GC-FID Chromatography SG-Std REG- insoluble ISTD2 SG

7. STD-SG Insoluble-B100soy STD:Di-;Tri- GC-FID Chromatography

8. Quantity of SG in Biodiesel Area SG Conc.(mg/l)SG REG 2665630307.98 61.6%(purity) Insoluble-SBO 91906.3210.6227510 ppm Insoluble-CSO 13304.861.531302 ppm B100-SBO (after filtration) 13016.111.5104 ppm B100-CSO ( after filtration) 23380.212.7219 ppm

9. Future Work Insoluble formation on SBO-based biodiesel (NextDiesel) Quantify the SG in biodiesel using GC-FID

10. GC-FID Chromatography: REG

11. Table 1. Sterol composition in some seed oils of commercial importance (mg/Kg). Corn oilcottonseedolivepalmrapeseedsafflowersoybeansunflower cholesterol---26---- campesterol2691170283581530452720313 stigmasterol7024214204-313720313 β-sitosterol77223961131018943549180919082352 Δ5-avenasterol46885295112235108156 Δ7-stigmastenol117-5825306696108588 Δ7-avenasterol-----10436156 brassicasterol----612--- other-----69-39 Data from Gunstone, F.D., Harwood, J.L. and Padley, F.B. The Lipid Handbook (Second Edition) (Chapman & Hall, London) (1994).