1. MBV2010/BIO2140 Colloquium, February 19

2. RULES Multiple choice Only one correct answer 30-60 seconds to answer No textbook, computer, mobile phone, please There will be a prize for the group with the most correct answers.

3. Example Below is a series of equations. Which numbers would fit logically into the last equation ? (13 – 4) : 9 = 1(27 – 9) : 9 = 2 (87 – 15) : 9 = 8(68 - ??) : 9 = ?? A(68 - 14) : 9 = 6 B (68 - 5) : 9 = 7 C (68 - 23) : 9 = 5 D (68 - 32) : 9 = 4 0

4. Example Below is a series of equations. Which numbers would fit logically into the last equation ? (13 – 4) : 9 = 1(27 – 9) : 9 = 2 (87 – 15) : 9 = 8(68 - ??) : 9 = ?? 0

5. Dideoxynucleotides are used in A DNA sequencing B Cloning of DNA C PCR D Southern hybridization 1

6. Dideoxynucleotides are used in 1

7. Ribosomal RNAs A code for ribosomal proteins B function in transcription of genes C are absent from mitochondria D function in protein synthesis 2

8. Ribosomal RNAs 2

9. The smallest human chromosome has a size of A 32 x 10 6 bp B 41 x 10 6 bp C 47 x 10 6 bp D 58 x 10 6 bp 3

10. The smallest human chromosome has a size of 3

11. The polymerase chain reaction (PCR) is used for A amplifying proteins B copying plasmids C copying RNA D amplifying DNA 4

12. The polymerase chain reaction (PCR) is used for 4

13. DNA ligases A catalyze the formation of ß-N- glycosidic bonds B play a role in transcription of DNA C catalyze the formation of phosphodiester bonds D catalyze the formation of hydrogen bonds 5

14. DNA ligases 5

15. Van der Waal’s forces A are attractive forces between atoms B are 10 times weaker than covalent bonds C are strongest at an atom distance of ≈ 0.2 nm D play a role in stabilization of the DNA double helix 6

16. Van der Waal’s forces 6

17. What is shown in this figure? A a random RNA structure B a microRNA structure C a small nuclear RNA structure D a tRNA structure 7

18. What is shown in this figure? 7

19. Protein structures can be determined by A mass spectrometry B 2D-polyacrylamide gel electrophoresis C x-ray diffraction D liquid chromatography 8

20. Protein structures can be determined by 8

21. The amino acid sequence of proteins can be determined by A mass spectrometry B 2D-polyacrylamide gel electrophoresis C x-ray diffraction D liquid chromatography 9

22. The amino acid sequence of proteins can be determined by 9

23. Centromeres A are present in all chromosomes B are normally in the center of chromosomes C are nucleosome-free sequences D normally contain repetitive DNA 10

24. Centromeres 10

25. SWI/SNF is a A histone modification complex B nucleosome remodeling complex C nucleosome assembly complex D none of the above 11

26. SWI/SNF is a 11

27. What is shown in this figure? A histone modifications in telomeres B histone modifications in protein-coding genes C replacement of histone H3 D replacement of histone H2A 12

28. What is shown in this figure? 12

29. The average size of genes in bacterial genomes is 13 A 1000 – 1500 bp B 500 – 1000 bp C 3000 – 4000 bp D 1500 – 3000 bp

30. The average size of genes in bacterial genomes is 13 Table 8.2 part 1 of 2 Genomes 3 (© Garland Science 2007) Vibrio cholerae Deinococcus radiodurans (Conan the bacterium)

31. What is the role of the SeqA protein in E. coli ? 14 A initiate replication B prevent replication C break hydrogen bonds D relieve topological stress in DNA

32. What is the role of the SeqA protein in E. coli ? 14 SeqA SeqA binds to hemimethylated DNA CH 3

33. When in a cell cycle is the eukaryotic origin recognition complex bound to DNA? 15 A in G 1, S, and G 2 B in S, G 2, and M C in G 2, M, and G 1 D only at the end of G 1

34. When in a cell cycle is the eukaryotic origin recognition complex bound to DNA? 15

35. Which of the following proteins is involved in initiation of replication in the nucleus? 16 A DnaA B Type II topoisomerases C Cdc6 D Cdc45

36. Which of the following proteins is involved in initiation of replication in the nucleus? 16

37. Replication protein A (RPA) is a 17 A helicase B topoisomerase C single-strand binding protein D DNA polymerase

38. Replication protein A (RPA) is a 17

39. The proliferating cell nuclear antigen (PCNA) is involved in 18 A cell division B nucleosome assembly C genome replication D formation of heterochromatin

40. The proliferating cell nuclear antigen (PCNA) is involved in 18