1. Cell Membranes are Selectively Permeable

2. What can pass? Water and other smaller molecules Water and other smaller molecules Gases like oxygen, carbon dioxide, and nitrogen Gases like oxygen, carbon dioxide, and nitrogen Small polar molecules Small polar molecules Larger non-polar molecules pass through more slowly Larger non-polar molecules pass through more slowly

3. Diffusion Random motion of particles powers diffusion- kinetic energy Random motion of particles powers diffusion- kinetic energy Particles move from an area of higher concentration to an area of lower concentration ultimately reaching equilibrium Particles move from an area of higher concentration to an area of lower concentration ultimately reaching equilibrium

5. http://www.biosci.ohiou.edu/introbioslab/ Bios170/diffusion/Diffusion.html

6. Rate of Diffusion Depends upon Depends upon  Temperature  Size of molecules  Electrical charges  Concentration gradient

7. Dialysis Diffusion of a solute across a selectively permeable membrane Diffusion of a solute across a selectively permeable membrane Useful when kidneys do not function properly; blood can be cleaned by kidney dialysis Useful when kidneys do not function properly; blood can be cleaned by kidney dialysis

8. Kidney dialysis machines were once not very common. Now, patients can find one in most every community.

9. Osmosis Diffusion of water across a selectively permeable membrane Diffusion of water across a selectively permeable membrane Osmotic pressure is the tendency of water to move into that solution. Osmotic pressure is the tendency of water to move into that solution.

12. Isotonic solution Isotonic- when the solution outside the cell is of equal concentration of solute as that inside the cell Isotonic- when the solution outside the cell is of equal concentration of solute as that inside the cell Solute concentration of solution equal to that of cell Solute concentration of solution equal to that of cell No net water movement No net water movement

13. Hypotonic solution Solute concentration of solution lower than cell Solute concentration of solution lower than cell Less dissolved particles outside of cell than inside of cell Less dissolved particles outside of cell than inside of cell Hypo = less, under (think hypodermic, hypothermia); Tonic = dissolved particles Hypo = less, under (think hypodermic, hypothermia); Tonic = dissolved particles Water moves into cell from solution Water moves into cell from solution Cell expands (and may burst) Cell expands (and may burst)

14. Hypertonic solution Solute concentration of solution higher than cell Solute concentration of solution higher than cell More dissolved particles outside of cell than inside of cell More dissolved particles outside of cell than inside of cell Higher water concentration inside cell Higher water concentration inside cell Water moves out of cell Water moves out of cell

15. What about human cells? Human cells are isotonic with a 0.9% sodium chloride solution. Human cells are isotonic with a 0.9% sodium chloride solution.

17. Osmotic pressure Solutions that are hypertonic have a higher osmotic pressure than the cells Solutions that are hypertonic have a higher osmotic pressure than the cells Solutions that are hypotonic have a lower osmotic pressure than the cells Solutions that are hypotonic have a lower osmotic pressure than the cells Remember that osmosis is the movement of water Remember that osmosis is the movement of water

19. Who is this man?

21. Osmotic Pressure changes Cell Size Plasmolysis- cell contents shrink away from its cell wall caused by a hypertonic solution Plasmolysis- cell contents shrink away from its cell wall caused by a hypertonic solution Crenation- cell shrinks caused by a hypertonic solution Crenation- cell shrinks caused by a hypertonic solution Cytolysis- cell contents swell and burst the cell caused by a hypotonic solution Cytolysis- cell contents swell and burst the cell caused by a hypotonic solution

22. plasmolysi s

24. crenation

25. Turgor Pressure The pressure that a plant cell places on its cell wall The pressure that a plant cell places on its cell wall Caused by the plant’s cellular environment being almost always hypotonic Caused by the plant’s cellular environment being almost always hypotonic Turgor provides structural support in non-woody plants Turgor provides structural support in non-woody plants

29. Aquaporin-1 Water sometimes seems to come into the cell faster than osmosis can account for Water sometimes seems to come into the cell faster than osmosis can account for Aquaporin-1 is an integral protein that acts as a gated water channel Aquaporin-1 is an integral protein that acts as a gated water channel Aquaporin-1 allows water to pass through but not protons Aquaporin-1 allows water to pass through but not protons

31. Question for Experts- (that’s you) Large molecules cannot move easily through the cell’s selectively permeable membrane. Ions cannot cross a lipid bilayer. Why is this advantageous to the cell? Large molecules cannot move easily through the cell’s selectively permeable membrane. Ions cannot cross a lipid bilayer. Why is this advantageous to the cell?

32. Carrier Mediated Transport Two forms Two forms  Facilitated diffusion  Still diffusion- down the gradient  Active transport  Energy requirement

33. Facilitated Diffusion Occurs down a concentration gradient Occurs down a concentration gradient Membrane can be made permeable to certain molecules by carrier or transport proteins Membrane can be made permeable to certain molecules by carrier or transport proteins

39. Carrier Mediated Active Transport Against diffusion flow- from low concentration to higher Against diffusion flow- from low concentration to higher Must use an energy source like ATP Must use an energy source like ATP

40. Sodium Potassium Pumps Found in most animal cells Found in most animal cells Exchange sodium ions (Na + ) on inside of cell for potassium ions (K + ) on outside of cell Exchange sodium ions (Na + ) on inside of cell for potassium ions (K + ) on outside of cell Usually 2 potassiums for three sodiums Usually 2 potassiums for three sodiums

41. Sodium Potassium Pump

42. Electrical Gradient When the concentration of net charges in one area is different than another When the concentration of net charges in one area is different than another The difference in the concentration of electrical charges drives ions across the membrane The difference in the concentration of electrical charges drives ions across the membrane Net “+” Net “-”

43. Cotransport Electrochemical gradient- higher concentration of electrical charges and molecules in one place than in another Electrochemical gradient- higher concentration of electrical charges and molecules in one place than in another Moving down the gradient can supply enough energy to push a molecule through a protein transport back against the gradient Moving down the gradient can supply enough energy to push a molecule through a protein transport back against the gradient

47. Energy Requirement for Transport Generally speaking, Facilitated Diffusion is said to require no energy to move molecules across the membrane Generally speaking, Facilitated Diffusion is said to require no energy to move molecules across the membrane In reality, the energy of diffusion is what powers this transport In reality, the energy of diffusion is what powers this transport In active transport, some other type of energy is required to make the transport work against the concentration gradient In active transport, some other type of energy is required to make the transport work against the concentration gradient

48. Endocytosis Three types Three types  Pinocytosis- pino “to drink”; cyto “cell”  Phagocytosis- phago “to eat”  Receptor-mediated endocytosis

50. Pinocytosis Cell takes in dissolved materials in the form of fluids; so you could say fluids or very small particles Cell takes in dissolved materials in the form of fluids; so you could say fluids or very small particles

51. PINOCYTOSIS

52. PINOCYTIC VESICLES FORMING

53. Phagocytosis Cell can ingest large particles by wrapping the cell membrane around the particle and taking it into the cell Cell can ingest large particles by wrapping the cell membrane around the particle and taking it into the cell Cell takes in food and bacteria Cell takes in food and bacteria

56. Receptor Mediated Endocytosis Involves bonding of receptor proteins to the material to be ingested Involves bonding of receptor proteins to the material to be ingested

57. Molecules bind to receptors called ligands concentrated in coated pits Molecules bind to receptors called ligands concentrated in coated pits  Coated pits are coated by the protein Clathrin The pits move inwards via endocytosis and are then termed coated vesicles The pits move inwards via endocytosis and are then termed coated vesicles Receptor Mediated Endocytosis

59. 1 2 1.Coated pit- coated with clathrin 2.Coated vesicle

60. The coated vesicle ultimately becomes an endosome when the coating is lost The coated vesicle ultimately becomes an endosome when the coating is lost Cholesterol is taken into cells by this pathway Cholesterol is taken into cells by this pathway Receptor Mediated Endocytosis

62. Brown and Goldstein- 1985 Nobel Prize Brown and Goldstein- 1985 Nobel Prize Receptor Mediated Endocytosis Michael S. Brown (right) and Joseph L. Goldstein (left) are currently Regental Professors in the Department of Molecular Genetics at the University of Texas Southwestern Medical Center in Dallas where they have worked together since 1972

64. Drs. Brown and Goldstein personify the team dynamic at the heart of UT Southwestern’s approach to research. They shared the 1985 Nobel Prize for their discovery of the underlying mechanisms of cholesterol metabolism. Their findings led to the development of statin drugs, the cholesterol-lowering compounds that today are used by 16 million Americans and are the most widely prescribed medications in the United States. And their discovery is improving more lives every year. New federal cholesterol guidelines will triple the number of Americans taking statin drugs to lower their cholesterol, reducing the risk of heart disease and stroke for countless people.BrownGoldstein