1. Avon Longitudinal Study of Parents and Children (ALSPAC) I4C Barcelona, Spain 2011 Update: Progress to date Cohort summary  Years of recruitment – 1990-2 in pregnancy  Number of live births at baseline 14062  Follow up status currently being contacted at age 20 Acknowledgements We are extremely grateful to all the families who took part in this study, the midwives for their help in recruiting them, and the whole ALSPAC team, which includes interviewers, computer and laboratory technicians, clerical workers, research scientists, volunteers, managers, receptionists and nurses. The UK Medical Research Council, the Wellcome Trust and the University of Bristol currently provide core support for ALSPAC.  For further details see the website : www. Bristol.ac.uk/alspac  Please note that the numbers of samples available was correct at 23.2.10. Jean Golding and Colleagues, Centre for Child and Adolescent Health, School for Social and Community Medicine, University of Bristol, Bristol, UK ENVIRONMENTAL SAMPLES COLLECTED ( YES/NO) TIMING OF SAMPLE COLLECTION (weeks gestation / months of age) LOCATION OF SAMPLE COLLECTION (e.g. child’s bedroom) CONDITIONS OF STORAGEMONTHS SINCE STORED TOTAL NUMBER OF SAMPLES COLLECTED NUMBER OF SAMPLES AVAILABLE SEPT 2011 PrenatalFirst year of life Indoor air sample Y(a)study homes monitored over a 12 month period (b) - (a)Approximately first 6 months (b) 1 st year (a)Not stored but analysed for NO2, formaldehyde, total VOCs, undecane, bacteria, (b) NO2 indoors and out DNA(a)150 homes, many sampling points per home (a) 1200 indoors, 500 of these outdoors DNA Dwelling dust collection N Soil sample N Drinking water N Hair sample from pets N Geographic Information Systems (GIS): Describe type of data collected, source of data, other relevant information. The study postcodes have been used for a variety of projects including the mapping of accidents and distance from overhead power lines.  Population sample Update: Primary outcomes  Cases of childhood cancer up until the age of 14 have been linked. BIOLOGICAL SAMPLES TIMING ( Indicate YES (Y) or NO (N) AND weeks gestation/months of age) CONDITIONS OF STORAGE MONTHS SINCE STORED BRIEF DESCRIPTION OF COLLECTION METHOD TOTAL NUMBER OF SAMPLES COLLECTED NUMBER OF SAMPLES AVAILABLE SEPT 2011 Prenatal (Mother) At birth (Mother/Child) First year of life (Child) No of individuals for whom samples are available Blood YSee Cord blood below Collected but no samples left (a)Prenatal: Whole blood EDTA; -20 (b)Red cells EDTA; -70 (c)Freeze dried plasma; room temperature (d)Plasma; -20/-70 (e)Red cells hep; -70 (f)White cells hep; -70 240Collected by midwife during clinical examination and sent to ALSPAC laboratories (a)7494 (b) 1613 (c) 1945 x 0.5ml; 339 x 1ml (d) 8103 x 1ml [up to 6 aliquots]; 524 x 0.5ml [up to 5 aliquots] (e) 2488 (f)2629 Serum YNY240(a)As above stored at -20 (b)Collected from what was left after AFP test (c) Collected from sample stored for rubella testing (a)7153 x 1ml [up to 3 aliquots] (b)4466 x 0.5-1ml [up to 3 aliquots] (c) 5605 x 0.5 -1ml Urine YNN240Collected as a midstream specimen and sent to the ALSPAC laboratories where it was aliquoted and stored at -20; up to 5 aliquots per woman. 10939 mothers Saliva NNN Buccal swab NNN Vaginal swab NNN Cord blood -YN(a) red cells EDTA at -70; (b) freeze dried plasma at room temp (c) plasma at -70; (d) red cells hep at -70; (e) serum at -20; 240(a)263 (b)144 x 1ml and 1898 at 0.5ml (c)4976 x 1ml, 1594 x 0.5ml. (d)1725 (e)6665 x 1ml Blood spots YYNStored -20240(a) Maternal antenatal in heparin (b) Cord blood in heparin (a) Up to 10 spots for 5687 women (b) Up to 5 spots per child for 9695 children. Placenta -Y-(a)The whole placenta stored in formalin at room temperature; (b)Umbilical cord stored at -20 240(a)Collected by midwives at delivery and put straight into formalin (a) Umbilical cord: immediately frozen but later thawed and sliced into 4 aliquots, then stored at -20 (a)8933 (b) 6283 Meconium -NN Hair sample NNYStored at room temp5076 Nail sample (Toes) NNYStored at room temp4945 Breast milk -NN Milk teeth NNYStored at -204168Up to 4 teeth per child4168