1. Example2: Building a protein from amino acids: Substrate: Amino acids Product: The protein

2. Beginning substance(s) Ending substances Reactant(s)Products Substrate(s) Notes We define: Chemical reaction: A process where atoms and bonds are re- arranged.

3. Consider the Benedict result from the four test tubes: (1. water, 2. starch, 3. saliva, 4. saliva + starch) 1) Which one showed presence of sugar? Why not in the others? 2) What was the purpose of dipping in warm water? 3) What was the purpose of the boiling water in the end?

4. Enzyme notes – cont.

5. Digestion by saliva Amylase Starch Glucose Example1: Substrate: Starch (Amylose) Products: Glucose molecules

6. Process of Enzyme Action: Reactants Products s l o w Substrates fast! Enzyme

7. Process of Enzyme Action: Binding to Active Site complex Product release

8. Process of Enzyme Action: Notes Step 1: Enzyme binds substrate Step 2: Enzyme and Substrate form a complex. Step 3: Changes occur in substrate. Step 4: Release of products, enzyme can start again. fast!

9. Notes Process of Enzyme Action: 1. Binding to Active Site 2. Temporary Enzyme- Substrate Complex 3. Product Release1 4. Product Release2

10. Enzyme Animation Course Website Starch digestion

11. Amylase (enzyme) Amylose / Starch (Substrate)

13. Fragmented polysaccharide (Product)

14. 1)What is the relationship between: a. enzyme, protein b. enzyme, amino acids c. amylase, enzyme d. protein, amino acids 2) Arrange the four terms in a brace map, using ‘our’ arrows. enzyme, protein, amino acids, amylase. 09.07.11 Journal 2