1. Leafy vegetables WP2: Characterisation and Regeneration WP-Leader: Andreas Börner, IPK Gatersleben, Germany

2. Characterization Descriptors Regeneration WP2 regeneration & characterization Year 4 Year 3 Year 2 Year 1 WP activities WP2: Characterisation and Regeneration 128314646182112797Characterization 1254334020149931Regeneration total rucolavaleria- nella chicoryspinachlettuce

3. WP2: Characterisation and Regeneration First year: Milestones & Deliverables D07 – D1145 Regeneration and characterisation for all crops started 12 D12- D15 D06 Deliverable # Start made developing minimum descriptors for the minor leafy vegetables 02 Material for regeneration and characterisation of the four crops selected 06 Minimum descriptors for the minor leafy vegetables concluded 06 Regeneration of some crops started pending the growing season 06 All accessions regenerated48 M&D descriptionMonth All accessions characterised

4. WP2: Characterisation and Regeneration First year: Milestones & Deliverables 24 Regeneration and characterisation for all crops started 12 D07- D11 D06 Deliverable # Start made developing minimum descriptors for the minor leafy vegetables 02 Material for regeneration and characterisation of the four crops selected 06 Minimum descriptors for the minor leafy vegetables concluded 06 Regeneration of some crops started pending the growing season 06 50% of all accessions characterised22 All accessions characterised45 M&D descriptionMonth 50% of all accessions regenerated D12- D15 All accessions regenerated 48

5. WP2: Characterisation and Regeneration Descriptors Feldsalat (Valerianella spp.) / corn salad (Valerianella spp.) Passportdaten – passport data Aussaatdatum – sowing date Aufgangsdatum – emerging date Datum der kommerziellen Reife – date of commercial ripeness Schossen - bolting Blühdatum – flowering time Erntedatum – harvest date Erntemenge – yield TKM – 1000 grain weight Pflanzenhöhe (cm) – plant height (cm) Wuchsform zur kommerziellen Reife – growth habit at commercial ripeness 1=waagrecht – horizontal 3=halb-aufrecht – semi-erect 5=aufrecht – erect Blattform (Abb. 1) – leaf shape (fig. 1) 1=elliptisch – elliptic 2=breit spatelförmig – broad spatulate 3=schmal spatelförmig – narrow spatulate Blattrand – leaf margin 1=ganzrandig, ungeteilt – entire 2=gezähnt – dentate Blattfarbe – colour of leaves 3=hellgrün – light green 5=grün – green 7=dunkelgrün – dark green Blattfarbe – colour of leaves 3=hellgrün – light green 5=grün – green 7=dunkelgrün – dark green Blattglanz – leaf glossiness 3=gering – weak 5=mittel – medium 7=stark – strong Blattblasigkeit – leaf blistering 3=gering – weak 5=mittel – medium 7=stark – strong Abb. 1: Blattform – fig. 1: leaf shape (source: UPOV, Guidelines for corn salad, 2006)

6. WP2: Characterisation and Regeneration Rucola (Eruca spp.) / rocket (Eruca spp.) Passportdaten – passport data Aussaatdatum – sowing date Aufgangsdatum – emerging date Datum der kommerziellen Reife – date of commercial ripeness Schossen - bolting Blühdatum – flowering time Erntedatum – harvest date Erntemenge – yield TKM – 1000 grain weight Pflanzenhöhe (cm) – plant height (cm) Wuchsform zur kommerziellen Reife – growth habit at commercial ripeness 1=halb-aufrecht – semi-erect 2=aufrecht – erect Blattform (Abb. 1) – leaf shape (fig. 1) 1=rundlich – round 2=elliptisch – elliptic 3=verkehrt eiförmig – obovate 4=spatelförmig – spatulate 5=eiförmig – ovate 6=lanzettlich – lanceolate 7=länglich, rechteckig – oblong Blattrand (Abb. 2) – leaf margin (fig. 2) 1=annähernd ganzrandig – nearly entire 2=leicht gebuchtet – slightly waved 3=tief gebuchtet, fiederteilig – deep sinuate, pinnatipartite Blattfarbe – colour of leaves 1=hellgrün – light green 2=grün – green 3=dunkelgrün – dark green 4=violett – violet Blütenfarbe – colour of flowers 1=weiß – white 2=cremefarben – creamy 3=schwefelgelb – sulphureous 4=gelb – yellow Äderung der Blüte – venation of the petals 0=fehlend – absent 1=vorhanden – present Abb. 1: Blattform – fig. 1: leaf shape (source: IPGRI, Descrittori per la Rucola, 2002) Abb. 2: Blattrand – fig. 2: leaf margin (source: IPGRI, Descrittori per la Rucola, 2002) Descriptors

7. WP2: Characterisation and Regeneration Characterisation PartnerLettuceSpinachChicoryValerianella Rucola P0 CGN 60 - - - 5 P1 HRI 30 30 - - - P2 GEVES - - 75 - - P3 IPK500 - - 29 134 P4 Palacky 30 - - - - P5 NGB - 70 - 8 7 P6 INRA - - - - - P7 Arche Noah 60 12 - - - P8 ProspecieRara - -100 - - P9 USOVA - - - 9 - P10 HDRA 17 - - - - P12 UNIBO - - - - - P13 AIS100 - 7 - -

8. WP2: Characterisation and Regeneration Regeneration PartnerLettuceSpinachChicoryValerianella Rucola P0 CGN 60 15 - - 5 P1 HRI 60 15 18 - 4 P2 GEVES 8 - 60 8 - P3 IPK320 - - 10 20 P4 Palacky - - - - - P5 NGB 6 7 - 5 4 P6 INRA300 - - - - P7 Arche Noah 60 12 16 8 - P8 ProspecieRara - -100 - - P9 USOVA - - - 9 - P10 HDRA 17 - - - - P12 UNIBO - - - - - P13 AIS100 - 7 - -

9. WP2: Characterisation and Regeneration Characterisation PartnerLettuceSpinachChicoryValerianella Rucola P0 CGN 60 (150) - - - 5 (0) P1 HRI 30 (?) 30 (?) - - - P2 GEVES - - 75 (25?) - - P3 IPK500 (150) - - 29 (10) 134 (40) P4 Palacky 30 (15) - - - - P5 NGB - 70 (70) - 8 (0) 7 (0) P6 INRA - - - - - P7 Arche Noah 60 (37) 12 (0) - - - P8 ProspecieRara - -100 (25) - - P9 USOVA - - - 9 (9) - P10 HDRA 17 (?) - - - - P12 UNIBO - - - - - P13 AIS100 (100) - 7 (0) - -

10. WP2: Characterisation and Regeneration Regeneration PartnerLettuceSpinachChicoryValerianella Rucola P0 CGN 60 (70) 15 (0) - - 5 (0) P1 HRI 60 (20) 15 (5) 18 (6) - 4 (0) P2 GEVES 8 (?) - 60 (?) 8 (?) - P3 IPK320 (80) - - 10 (3) 20 (8) P4 Palacky - - - - - P5 NGB 6 (?) 7 (7) - 5 (0) 4 (0) P6 INRA300 (75) - - - - P7 Arche Noah 60 (37) 12 (0) 16 (?) 8 (?) - P8 ProspecieRara - -100 (25) - - P9 USOVA - - - 9 (9) - P10 HDRA 17 (17) - - - - P12 UNIBO - - - - - P13 AIS100 (100) - 7 (0) - -