1. Antioxidant activities and reactive oxygen species in flag leaves of Fusarium-inoculated wheat genotypes Szeged – Timişoara axis for the safe food and feed SZETISA1 HURO/0901/147/2.2.2 Ágnes Gallé Department of Plant Biology University of Szeged 2nd Progress Report Meeting Szeged, 22-23 September, 2011 Reporting period: 01.06. 2011 – 30.09. 2011 www.huro-cbc.eu www.hungary-romania-cbc.eu Disclaimer The content of this page does not necessarily represent the official position of the European Union. Two countries, one goal, joint success!

2. Fusarium head blight (FHB) is an important disease of wheat and has worldwide distribution, which results in yield loss and mycotoxin contamination of grain when the disease is caused by a mycotoxin-producing Fusarium species. Fusarium graminearum and F. culmorum are the Fusarium species that most commonly cause FHB of wheat and both can produce trichothecene mycotoxins, predominantly deoxynivalenol (DON, also known as vomitoxin), zearalenone, nivalenol, dideoxynivalenol, 3-acetyldeoxynivalenol Fusarium graminearum Fusarium culmorum

3. Vomitoxin, also known as deoxynivalenol (DON), is a type B trichothecene, an epoxy-sesquiterpeoid. When compared to other trichothecene mycotoxins which can form in grains and forages, vomitoxin is relatively mild. Vomitoxin belongs to a class of mycotoxins which are strong protein inhibitors. Inhibition of protein synthesis following exposure to vomitoxin causes the brain to increase its uptake of the amino acid tryptophan and, in turn, its synthesis of serotonin. Increased levels of serotonin are believed to be responsible for the anorexic effects of DON and other tricothecenes. Irritation of the gastrointestinal tract may also play a role in reducing feed intake.

4. Zearalenone is also a trichothecene mycotoxin, and has estrogen effect, and can cause inflame in the uterus in swine. Nivalenol was shown to inhibit protein synthesis in rabbit reticulocytes in vitro, with an ID50 of 2.5 mg/ml, probably by impairment of the ribosomal function. Nivalenol inhibited the synthesis of nucleic acids in vitro (Ueno and Fukushima, 1968). This response appeared at doses much higher than the inhibition of protein synthesis. The mechanism of nucleic acid inhibition is not known.

5. Before inoculation sampling (ROS and total antioxidant capacity) anthesis inoculation SAMPLING Dough stagePremilk stageMilk stage GK Mini Manó/Nobeokabozu genetic mapping population (Prof. Mesterházy Ákos ) Nobeokabozu: FHB resistant line A recombinant line from China: ‘WSY’, Wangshubai/Sumai3/Yangangfanzhou GK Mini Manó Arthur 71 / Sava // Rusalka / NS 171.2 /3/ F 30.74 Resistant line to e.g. leaf rust Plant material

8. Enzymatic antioxidants

9. Peroxidases (POX; EC 1.11.1.7) are oxido-reductive enzymes that participate in the wall-building processes such as oxidation of phenols, suberization, and lignification of host plant cells during the defense reaction against pathogenic agents. Accumulation of lignin and phenolic compounds have been correlated with disease resistance in a number of plant–pathogen interactions. These include wheat, tomato and rice. Enhanced POX activity has been correlated with resistance in rice, wheat, barley and sugarcane following the inoculation with phytopathogens. POX activity was rather delayed or remained unchanged during the compatible interaction in susceptible plants..

10. Peroxidase specific activity in heads of wheat cultivars. C, non-inoculated control; I, inoculated plants. (F. graminearum)

15. Some protein families (examples) which were induced during Fusarium infection : Zhou et al 2005 Proteins with an antioxidant function: superoxide dismutase, dehydroascorbate reductase, and glutathione S-transferases (GSTs) Expression of proteins with highest similarity to dehydroascorbate reductase and TaGSTF5 (a glutathione S- transferase) differed following FHB infection in susceptible and resistant cultivars.

17. Parameter561474501483488 POD SOD KAT GR GST Total ROS Total antioxidant capacity FUSARIUM CULMORUM

18. Parameter561474501483488 POD SOD KAT GR GST Total ROS Total antioxidant capacity FUSARIUM GRAMINEARUM

19. Parameter561474501483488 POD SOD KAT GR GST Total ROS Total antioxidant capacity CONTROL

20. Conclusions: The Fusarium inoculation on the ear influenced the hole antioxidant system in the flag leaves of wheat in dough stage! There were differences between the resistant and the sensitive cultivars in POD activity inductions. BUT according our measurements differences were detecable in control circumstances. And the amount of ROS, antioxidant capacity and the GST activity correlated with the resistance.

21. Thank you for your attention!