1. Lienhard Pagel 4.6.2007 Flow injection analysis realized using PCBs S. Gassmann, L. Pagel Faculty of computer since and electronics Institute of Electronic Appliances and Circuits, University of Rostock, Rostock, Germany

2. Lienhard Pagel 4.6.2007 Micro fluidics in PCB technology

3. Lienhard Pagel 4.6.2007 University of Rostock PCB-Fluidics

4. Lienhard Pagel 4.6.2007 Fluidic PCB-Technology normal PCB production drilling milling

5. Lienhard Pagel 4.6.2007 Fluidic PCB-Technology normal PCB production etching

6. Lienhard Pagel 4.6.2007 Fluidic PCB-Technology second PCB stacked copper lines build channels gluing

7. Lienhard Pagel 4.6.2007 Fluidic PCB-Technology tube connection channel view

8. Lienhard Pagel 4.6.2007 Fluidic PCB-Technology cross section of channel

9. Lienhard Pagel 4.6.2007 Cross section of channel wall epoxy copper (2) copper (1) channel

10. Lienhard Pagel 4.6.2007 µC controlled programmable micro pump  Electrical connector - Power 4 –7 V DC - I 2 C - Bus - programming  500µl/min Flow  150 mBar max Backpressure  over USB-I2C IF fully programmable

11. Lienhard Pagel 4.6.2007 Flow-Injection-Analysis quantitative measurement of components automated procedure short response times / high sample throughput usage in quality control / automated assays developed by J. Růžička and E.H. Hansen in the year 1975

12. Lienhard Pagel 4.6.2007 Flow-Injection-Analysis Sample Reaction coil Injection valveDetector Reagent Pump

13. Lienhard Pagel 4.6.2007 Reaction Detection of Fe 3+ Thiocyanate as reagent forms red colored Iron-Thiocyanate detection by absorption at 480 nm

14. Lienhard Pagel 4.6.2007 Why a FIA? All basic fluidic handling necessary - transportation of fluids - dosing of fluids - mixing - detecting - adoption to detection needed - electronics needed

15. Lienhard Pagel 4.6.2007 FIA in PCB-Technologie Principle reagent pump sample pump reaction coil injectiondetector 2 separate controllable pumps no injection valve optical detection photometrical 480 nm

16. Lienhard Pagel 4.6.2007 MIXING Flow pattern in 180° curves

17. Lienhard Pagel 4.6.2007 Simulation results of the used reaction channel Parameters: 2 pumps with constant flow of 0.2ml/min Fluidsimulation: FLOWORKS 789388 cells

18. Lienhard Pagel 4.6.2007 … and the reality

19. Lienhard Pagel 4.6.2007 Simulation results of the used reaction channel parameters: thermopneumatically actuated pumps with pulsing flow of 0.2ml/min, 789388 cells

20. Lienhard Pagel 4.6.2007 Simulation results of the reaction channel vector plot of the fluid velocity at the cross section of the channel after the second curve parameters: thermopneumatically actuated pumps with pulsing flow of 0.2ml/min, 789388 cells

21. Lienhard Pagel 4.6.2007 OPTICAL SENSOR OPTICAL SENSOR in FIA

22. Lienhard Pagel 4.6.2007 Optical Cell with air bubble (vector of the force)

23. Lienhard Pagel 4.6.2007 Realization 5 cm stack of four PCBs one movable layer of polyimid electronics on the back of the fourth PCB

24. Lienhard Pagel 4.6.2007 FIA Realization

25. Lienhard Pagel 4.6.2007 FIA in PCB with integrated electronics

26. Lienhard Pagel 4.6.2007 Signals in the photo detector in dependence on the concentration of Fe(III)ions

27. Lienhard Pagel 4.6.2007 PCB FIA facts Detection Fe 3+ Ions 0,16 mMol – 10 mMol reproducibility peak height 5%, peak area 10% Monolithic system consisting fluidics and electronics in one stack of PCBs.

28. Lienhard Pagel 4.6.2007 Thank you for your attention!