1. NI DAQ, Nanopositioner (Tilt mirror)  Reference: NARIONAL INSTRUMENT, “NI-DAQ 7, DAQmx C Reference Help ”, 2004 NARIONAL INSTRUMENT, “DAQ, 6534X User Manual”, 2001 nPoint, “C-300 Series Controller Manual”, Version 1.2, 2005 Control of the focal point location is done using a piezoelectric tilt mirror for the in plane motions

2. Specification MODELTILT STAGE ParameterTypicalMaximumUNIT Tilt range(mrad)8mrad Static stiffness4N/mm Integrated sensorStrain Gauge Resolution Resonant frequency1kHzunloaded InterfaceUSB MaterialAl

3. Application of Tilt mirror Trapping position control with tilt mirror Objective lens Tilt mirror –Controlling the position of the laser focus in two dimensional plane perpendicular to the beam axis –The tilt mirror is positioned at the conjugate plane of the objective back aperture to minimize the loss of energy caused due to the deflection of the tilt mirror

4. Point scan (20 Hz) Line scanning and circle scanning Application of Tilt Mirror (con’t) 2 point3 point circle Line

5. nPoint Digital Controller interface nPoint C300 USB V1.2.5 –Provides nanopositioning control capabilities –Graphical controls facilitate easy adjustment of control parameters, step-response verification and enabling of advanced control modes X, Y axis control

6. Interface of the Tilt mirror scanning User interface of optical tweezers control program

7. [7] Nanopositioner Control with NI DAQ 3D laser focus position control

8. [8] NI DAQ ? NI DAQ (National Instrument Data Acquisition) : Collecting and measuring the same kinds of electrical signals with analog-to-digital and/or digital devices plugged into a PC, and possibly generating control signals with digital-to-analog and/or digital devices in the same PC. PC-BASED DATA ACQUISITION Figure referred to www.ni.com  Two libraries for NI DAQ - Traditional NI DAQ, difficult to understand and implement - NIDAQmx: new library, easy to understand and implement

9. [9] PCI-6534 DAQ Device PCI - 6534 www.ni.com 32 (5 V TTL/CMOS) digital input/output lines 20 MHz (80 Mbytes/s) maximum transfer rate 8, 16, or 32-bit transfers Start and stop triggering, pattern and change detection 32 MB onboard memory per data path (group) (NI 6534 only) NI-DAQ driver simplifies configuration and measurements  Operating Systems Windows 2000/NT/XP/Me/9x Mac OS 9*  Recommended Software LabVIEW Measurement Studio for Visual  Other Compatible Software Visual Basic C/C++  Driver Software (included) NI-DAQ

10. [10] NI-DAQmx Key Concepts Task: A task is a collection of one or more channels, timing, triggering, and other properties that apply to the task itself. Conceptually, a task represents a measurement or generation you want to perform. Channel: Virtual channels are software entities that encapsulate the physical channel. The physical channel can be configured as data output channel or data input channel. Timing, Triggering: The timing section explains clocks. The triggering section goes over the triggers such as a Start Trigger and a Reference Trigger, Analog Edge Trigger, Digital Edge Trigger. Buffer: A buffer is a temporary storage in computer memory for acquired or to-be-generated samples.

11. [11] Basic NI DAQmx Functions for PCI-6534 Device Task Configuration/Control : DAQmxCreateTask (), DAQmxStartTask(), DAQmxStopTask(), DAQmxClearTask(), DAQmxIsTaskDone(), etc. Channel Creation : DAQmxCreateDOChan(), DAQmxCreateDIChan() Timing: DAQmxCfgSampClkTiming() Write: DAQmxWriteDigitalU8(), DAQmxWriteDigitalU16(), DAQmxWriteDigitalU32() Read: DAQmxReadDigitalU8(), DAQmxReadDigitalU16(), DAQmxReadDigitalU32()

12. [12] DAQ Flowcharts DAQmxCreateTask DAQmxCfgSampClkTiming DAQmxCreateDOChan Finite Sample Writing to DAQ device DAQmxWriteDigitalU16 DAQmxStartTask DAQmxStopTask DAQmxClearTask DAQmxCreateTask DAQmxCfgSampClkTiming DAQmxCreateDIChan Finite Sample reading from DAQ device DAQmxReadDigitalU16 DAQmxStartTask DAQmxStopTask DAQmxClearTask

13. [13] Example code (data output) Include “nidaqmx.lib” in the project setting

14. [14] Example code (data input) Include “nidaqmx.lib” in the project setting