1. ENZYMES 1- 2-

2. Enzymes lower the activation energy of reactions.

3. Substrate: Enzymes are specific. Active site:.

4. How does an enzyme work? Enzymes + substrate ES Enzyme + product What does rate of reaction mean? How is rate of reaction measured? A+BC+Enzyme Is this reaction a synthesis reaction or breaking down reaction? Enzyme

6. Structure of enzymes: Simple enzymes:

7. Complex enzymes: 1- proetein part+ (apoenzyme) Inorganic part (cofactor) Active enzyme 2- proetein part+ (apoenzyme) organic part (coenzyme) Active enzyme

8. Naming enzymes ------------ ase, e.g: What is the substarate of lipase? What kind of reaction is catayist by DNA polymerase? How about pepsin, amylase?

9. Factors that affect enzymatic activity 1- Temperature: What is the optimum temperature of a human body enzyme?

10. How does low temperature affect enzymatic activity? How does high temperature affect enzymatic activity?

11. 2- pH changes What is the optimum pH of pepsin? What would happen if you put an enzyme in acidic medium? What would happen if an enzyme is put in a basic solution?

12. 3- Substrate concentration Describe how does substrate concentration affect rate of reaction?

13. 4- Enzyme concentration How does enzyme concentration affect enzymetic activity? How is enzyme concentration increased?

14. 5- Inhibitor HL Inhibitors are the chemicals which bind active site of an enzyme. Eg: cyanade?

15. Graph? Enzyme inhibitor