1. Figure 2-3

2. Table 2-2

3. Figure 2-7 (1 of 3)

4. Figure 2-7 (2 of 3)

5. Figure 2-7 (3 of 3)

6. Figure 2-8 (2 of 6)

7. Figure 2-8 (3 of 6)

8. Figure 2-8 (4 of 6)

9. Figure 2-8 (5 of 6)

10. Figure 2-8 (6 of 6)

11. Figure 2-9 (2 of 7)

12. Figure 2-9 (3 of 7)

13. Figure 2-11 - Overview (1 of 3)

14. Figure 2-14

15. Figure 2-15

16. METABOLISM Energy Transformations –What determines how much energy is transferred? What factors can influence whether a chemical reaction (or set of chemical reactions) produces a product? What is the difference between an exergonic and an endergonic reaction? What does it mean when reactions are coupled? Where does the energy to make ATP come from? Why do we make ATP (  G= -30.9kJ/mole) rather than PEP (  G=- 60.9 kJ/mole)?

17. Compound  G o 'of phosphate hydrolysis (kJ/mol) Phosphoenolpyruvate (PEP)- 61.9 Phosphocreatine- 43.1 Pyrophosphate- 33.5 ATP (to ADP)- 30.5 Glucose-6-phosphate- 13.8 Glycerol-3-phosphate- 9.2 High Energy Compounds

18. Gibbs Free Energy Changes Rxn#EnzymeΔG°'(kJ/mol)ΔG(kJ/mol) 1Hexokinase-16.7-33.5 2Phosglucoisom+1.7-2.5 3Phosfructkinase-14.2-22.2 4Aldolase+23.9-1.3 5TriosphosIsom+7.6+2.5 6G-3-PDH+12.6-3.4 7Phosglyc kinase-37.6+2.6 8Phosglyc mutas+8.8+1.6 9Enolase+3.4-6.6 10Pyruvate kinase-62.8-33.4 12345671234567 8989 10 ΔG°‘ = under standard temperature and pressure with equal concentrations of reactants ΔG = non standardized conditions (physiological)

19. Figure 4-4 - Overview

20. Enzymes What kind of biomolecule is an enzyme? What does an enzyme do to make a reaction go faster? How do the substrates bind to the enzyme? What happens to the enzyme when the reaction is complete?

21. Figure 2-16

22. Figure 4-8

23. Table 4-3

24. Figure 2-17 Some Important Characteristics of Enzymes: Some enzymes must be “activated” before they can interact with their ligand

25. Figure 2-18 Some require a cofactor or coenzyme in order to make the reaction proceed Glucose +ATP Glucose-6-Phosphate hexokinase Mg 2+

26. Coenzyme Coenzymes: Pyruvate Lactate Lactate dehydrogenase NADH+ H + NAD +

27. Table 2-3

28. Figure 2-19 Succinate Fumarate Succinate dehydrogenase Malonate or oxaloacetate FAD+ FADH2

29. Figure 2-20a Glucose Glucose-6-phosphate ATP ADP Glycogen Glycolysis Glycogen phosphorylase

30. Figure 2-20b Glucose Glucose-6-phosphate ATP ADP hexokinase

31. Covalent modulation – generally an addition or removal of a phosphate group; can either increase or decrease the activity of the enzyme Triglyceride fatty acid + diglyceride Hormone sensitive lipase epinephrine 2 nd messenger activation of kinase P

32. Figure 2-21 Modulation by temperature

33. Modulation by pH – How? What is the mechanism? pH for Optimum Activity Enzyme pH Optimum Lipase (pancreas)8.0 Lipase (stomach)4.0 - 5.0 Lipase (castor oil)4.7 Pepsin1.5 - 1.6 Trypsin7.8 - 8.7 Urease7.0 Invertase4.5 Maltase6.1 - 6.8 Amylase (pancreas)6.7 - 7.0 Amylase (malt)4.6 - 5.2 Catalase7.0

34. Figure 2-22 Enzyme Given a set concentration of substrate, more enzyme makes a reaction proceed faster

35. Figure 2-23 enzyme With a fixed concentration of enzyme, increasing the substrate concentration will lead to the enzyme becoming saturated, and the reaction will go no faster (maximum rate).

36. Many enzymes can bind more than one substrate. How can we tell which substrate binds more efficiently? Determine Km (binding affinity)! *Rule = the smaller the Km, the tighter the binding

37. You have a mixture of several metabolic intermediates in a test tube. The intermediate compound A is the substrate of enzyme ZZ. Additional compounds include B, C, D, F, and G. Look at the following conditions and explain what is happening. Condition 1: A and B are in in the test tube in equal concentrations. A is found to occupy the active site 3X as often as B, but when both are present, the reaction rate slows down. Draw what the activity of the enzyme might look like in the presence of compound A, and then when A and B are both present. Condition 2: Enzyme ZZ is in the test tube with intermediates A, C and F. The activity of the enzyme is lower than expected. What is possibly happening and how would you determine whether your theory is true or not? Graph your experimental results. Condition 3. Enzyme ZZ is in the test tube with intermediates B and D. The activity is higher than expected. What is possibly happening and how would you determine whether your theory is true or not? Graph your experimental results. Activity of enzyme [Substrate] ?