1. Care and Use of the Microscope

2. Do’s and Don’ts Replace dust cover and put up after use
Hold with one hand on the arm and one on the base
Only use lens paper to clean microscope lens
Report any problems to me
Let the cord hang off the lab table
Drag the microscope across the lab table
Try to disassemble the microscope

5. Calculating Magnification
Two lenses
Objective lens
Ocular lens (eyepiece)
Usually 3-4
Common set: scanning power (4x), low-power (10x), high-power (40x), and oil immersion (100x)
Ocular lens
(10x)
Total Magnification= objective lens magnification x ocular lens magnification

6. Example Problem 1
Q: If the ocular lens has a power of 10x and the objective lens has a power of 100x, what is the total magnification?

7. Example Problem 1
Q: If the ocular lens has a power of 10x and the objective lens has a power of 100x, what is the total magnification?
A: Multiply the ocular power times the objective power
10x * 100x=1000x
Total Magnification=1000x

8. Example Problem 2
Q: If the ocular lens has a power of 5x and the objective lens has a power of 40x, what is the total magnification?

9. Example Problem 2
Q: If the ocular lens has a power of 5x and the objective lens has a power of 40x, what is the total magnification?
A: Multiply the ocular power times the objective power
5x * 40x=200x
Total Magnification=200x

10. Preparing a slide
((Didn’t use this in class this time, but I figured I’d leave it in))
To make a temporary wet mount slide
Get a clean slide
Place a tiny, thin piece of the specimen on the slide
Place a small drop of water over the specimen
Place a cover slip over the specimen
For oil emersion put a drop of oil on top of the cover slip

11. For this Lab Not making a wet mount
Remember when looking into the microscope to try adjusting the following to get a good view….
Coarse focus
Fine focus
Iris Diaphragm
Position of the slide
Level of magnification