1. Parts of the microscope Pamela Gonzalez 9 th grade Biology I

2. Objective After completing this tutorial, you should be able to identify and describe the parts and functions of the compound microscope with 90% accuracy. After completing this tutorial, you should be able to identify and describe the parts and functions of the compound microscope with 90% accuracy. TN Biology I state standards 1.0: The student will investigate the structures and functions of cell membranes, organelles, and component biomolecules as related to major cell processes by demonstrating appropriate use and care of compound light microscopes. TN Biology I state standards 1.0: The student will investigate the structures and functions of cell membranes, organelles, and component biomolecules as related to major cell processes by demonstrating appropriate use and care of compound light microscopes. NETSS Profile 9-12: 2: Make informed choices among technology systems, resources, and services. (1, 2) NETSS Profile 9-12: 2: Make informed choices among technology systems, resources, and services. (1, 2)

3. Directions Click on to take you to the previous slide. Click on to take you to the next slide. Click on to take you to your microscope menu. Click on to bring you back to these directions. This page shows the buttons that you will use to move around in this tutorial. The main menu provides a labeled picture of a microscope. Each label on the microscope is a link to more information on that part. Click each link on the menu before proceeding to the summary. Proceed to the summary from the microscope menu by clicking the next button. Get started by clicking the microscope menu button.

4. Microscope Menu eyepiece fine adjustment coarse adjustment base light source stage objectives arm

5. Eyepiece The eyepiece is the point where you look at your specimen. The eyepiece is the point where you look at your specimen. The eyepiece has a magnification of 10X. This is important to remember when calculating the total magnification. The eyepiece has a magnification of 10X. This is important to remember when calculating the total magnification.

6. Stage The stage where your slide is placed for viewing. The stage can be moved to locate specimens on the slide. The stage sits above the light source. The stage where your slide is placed for viewing. The stage can be moved to locate specimens on the slide. The stage sits above the light source. When placing a slide on the stage for viewing, remember that to put the stage at its lowest position to avoid accidentally breaking a slide.

7. Arm The arm acts as support for the microscope and connects the eyepiece and base. The arm acts as support for the microscope and connects the eyepiece and base. The arm is one of the parts you must hold properly secure the microscope while carrying it. The arm is one of the parts you must hold properly secure the microscope while carrying it.

8. Objective lenses Our microscopes have four objectives. The objectives are 4X, 10X, 40X and 100X. Our microscopes have four objectives. The objectives are 4X, 10X, 40X and 100X. The shortest objective is the lowest power. The shortest objective is the lowest power.

9. Coarse adjustment The course adjustment knob is the larger knob and is used first when bringing a specimen into focus. The course adjustment knob is the larger knob and is used first when bringing a specimen into focus. This moves the stage closer to the objective lens. Use caution to avoid breaking the slide when doing this. This moves the stage closer to the objective lens. Use caution to avoid breaking the slide when doing this.

10. Fine adjustment The fine adjustment knob is the smaller knob protruding from the coarse adjustment knob and is used to bring a clear image into sharper focus. The fine adjustment knob is the smaller knob protruding from the coarse adjustment knob and is used to bring a clear image into sharper focus. The fine adjustment knob is used second. The fine adjustment knob is used second.

11. Base The base acts as support. It is the bottom most part of the microscope. The base acts as support. It is the bottom most part of the microscope. The base, along with the arm, are used to safely carry the microscope to a table. The base, along with the arm, are used to safely carry the microscope to a table.

12. Light source Our microscopes use a light source. Our microscopes use a light source. The light source is a 110V light bulb instead of a mirror. The light source is a 110V light bulb instead of a mirror.

13. Summary The proper way to hold the microscope is to place one hand under the base and grasp the arm with the other hand. The proper way to hold the microscope is to place one hand under the base and grasp the arm with the other hand. The objectives provide different magnifications and length tells its strength of magnifications. The objectives provide different magnifications and length tells its strength of magnifications. When calculating the total magnification of what you are viewing, calculate it with the eyepiece magnification included. When calculating the total magnification of what you are viewing, calculate it with the eyepiece magnification included.

14. Quiz When you feel comfortable with the material covered on the parts of the microscope and their functions, click the next slide button to take the quiz. When you feel comfortable with the material covered on the parts of the microscope and their functions, click the next slide button to take the quiz. Read each question carefully and select the best answer. Click on your answer. Read each question carefully and select the best answer. Click on your answer. If you answer incorrectly, you can click back to the question to try again. If you answer incorrectly, you can click back to the question to try again. When you answer correctly, you can click to proceed to the next question. When you answer correctly, you can click to proceed to the next question.

15. Question 1 What two parts of the microscope are held when properly holding your compound microscope? A. Arm and eyepiece Arm and eyepiece Arm and eyepiece B. Stage and base Stage and base Stage and base C. Base and arm Base and arm Base and arm D. Base and objectives Base and objectives Base and objectives

16. Uh-oh

17. Wahoo!

18. Question 2 What magnification is the eyepiece? A. 5X 5X B. 10X 10X C. 15X 15X D. 20X 20X

19. Oh-no

20. Great job!

21. Question 3 Once a slide is on the stage, which adjustment focus is used first? A. Coarse adjustment Coarse adjustment Coarse adjustment B. Fine adjustment Fine adjustment Fine adjustment C. Objective Objective D. Eyepiece Eyepiece

22. Try again

23. Excellent

24. Question 4 Before placing a slide on the stage, you should do which of the following steps? A. Turn to the highest power objective Turn to the highest power objective Turn to the highest power objective B. Focus your eyepiece Focus your eyepiece Focus your eyepiece C. Place the stage at its lowest position Place the stage at its lowest position Place the stage at its lowest position D. Place the stage at its highest position Place the stage at its highest position Place the stage at its highest position

25. Oops

26. Perfect

27. Resources Miller, K. R. & Levine, J (2002). Biology. Miller, K. R. & Levine, J (2002). Biology. New Jersey: Pearson Education Inc. All pictures are courtesy of Microsoft Clipart. All pictures are courtesy of Microsoft Clipart.