2. In 1665, ________ ________ reported that living things were composed of little boxes or cells. 1673-1723, _______ ____ _______________– first to describe live microorganisms that he observed in teeth scrapings and pond water

3. The hypothesis that living organisms arise from nonliving matter is called _______________ _________________. According to spontaneous generation, a “_________ _______” forms life. The alternative hypothesis, that the living organisms arise from preexisting life, is called ______________. In 1858, Rudolf ____________ said that cells arise from preexisting cells. Cell Theory: All living things are composed of _________ and come from preexisting ___________.

4. Beginning in 1668, experiments were conducted to either prove or disprove spon gen. But before we get into that, let’s talk about experimental design. Some terms: Control – something that is used as a constant and unchanging standard of _______________ in an experiment Variable – factor, trait, condition, etc that ___________in an experiment Manipulated/Independent variable – variable that is changed (manipulated) by the scientist; only one of these in an experiment Responding/Dependent variable – variable that responds to and depends upon the ___________________ variable Experimental group - group in an experiment that receives the _____________________ variable Control group – group in an experiment that does NOT receive the _____________________ variable

5. Chemists working for LiveLonger Pharmaceuticals believe that they have developed a drug that will lengthen human life. This potential drug needs to be tested before LiveLonger can apply for a patent from the Food and Drug Administration for production of their life-lengthening drug. Research scientists from LiveLonger test the drug as follows: 5,000 individuals involved; 2500 60-year-old non-smoking men and 2500 60-year-old non-smoking women 50% are given a pill containing the drug; 50% are given a placebo Study conducted over a five-year period Controls? Manipulated variable? Responding variable? Experimental group? Control group? Experimental design (2:39)

6. ConditionsResults 3 sealed jarsNo maggots on the meat 3 open jarsMaggots on the meat 3 jars covered with fine net No maggots on the meat Where did the maggots come from? What was the purpose of the open jars? What was the manipulated variable? 1668: Francesco Redi (biogenesis) placed decaying meat in nine jars then made these observations:

8. ConditionsResults Nutrient broth boiled, poured into flasks, and then sealed Microbial growth Where did the microbes come from? What error did he make? 1745: John Needham (spon gen) put boiled nutrient broth into flasks.

9. ConditionsResults Nutrient broth placed in flask, sealed, then boiled No microbial growth Needham claims, “The ‘vital force’ was destroyed!” 1767: Lazzaro Spallanzani (biogenesis) boiled nutrient solutions in sealed flasks.

11. ConditionsResults Nutrient broth placed in flask, boiled, not sealed Microbial growth Nutrient broth placed in flask, sealed, then boiled No microbial growth Why the difference? 1861: Louis Pasteur (biogenesis) demonstrated that microorganisms are present in the air.

12. 1864-1865: Pasteur boiled broth in open swan-necked flasks and let the flasks sit for a __________. No __________ grew in the broth. He then broke off the necks and within a few hours the flasks were teeming with _________________.

13. Part Two: Microscopy History of the Microscope (4:40)

14. A ________ __________ has only one lens. ____ _______________ was obsessed with lens grinding. He ground single lenses that could magnify _____x; they were by far the ________ in the world at that time. Leeuwenhoek Video (2:00)

15. 1595: First ____________ microscope; made by Hans and Zaccharias Janssen _____ lenses: one at the top and one at the bottom Poor __________ and low __________, but it paved the way

16. In a ______________ microscope the image from the objective lens is ______________ again by the ocular lens. Total magnification = _____ lens  _____ lens

17. _______________ is the ability of the lenses to distinguish two points. A microscope with a resolving power of 4nm can distinguish between two points ≥ 4nm. If less than 4nm apart, then the two points appear as one. The better the ___________, the finer the detail. Visible light, because of its long _____________, limits light microscopes to _____x.

18. Uses _________ instead of ________. The shorter ____________ of electrons gives far greater ____________ than visible or UV light and can therefore show the smallest objects known.

19. Ultrathin sections of specimens must be used. Electrons pass through specimen, then an ____________________ lens to a screen or film. Internal chamber is a ___________. How a TEM works (1:48)

20. 10,000-100,000  (5,000,000x max) Resolution 2.5 nm Great for examining viruses and internal cellular structures

21. An electron gun produces a beam of ___________ that _________ the surface of a whole specimen.

22. 100-10,000x (300,000x max) Resolution 20 nm Great for examining surface features

23. Scanning tunneling microscope uses a metal __________ to __________ the surface of a specimen. Produces an image that reveals the ridges and depressions of individual _________ Resolution: 1/100 of an atom!! Most _____________ microscope on earth! “Molecular Man” Image made from scanning 28 CO molecules on platinum Individual silicon atoms

24. From the simple…. (couple of thousand $$) to the complex! (hundreds of thousands)