1. Label the parts on your microscope picture.
Eyepiece/ocular lens magnification = 10X

2. Label the parts on your microscope picture.
Eyepiece/ocular lens magnification = 10X
Arm-Support

3. Label the parts on your microscope picture.
Eyepiece/ocular lens magnification = 10X
Arm-Support
Stage - support

4. Label the parts on your microscope picture.
Eyepiece/ocular lens magnification = 10X
Arm-Support
Stage - support
Coarse adjust – general focus

5. Label the parts on your microscope picture.
Eyepiece/ocular lens magnification = 10X
Arm-Support
Stage - support
Coarse adjust – general focus
Fine Focus – high power focus

6. Label the parts on your microscope picture.
Eyepiece/ocular lens magnification = 10X
Arm-Support
Stage - support
Coarse adjust – general focus
Fine Focus – high power focus
Base - support

7. Label the parts on your microscope picture.
Eyepiece/ocular lens magnification = 10X
Arm-Support
Stage - support
Coarse adjust – general focus
Fine Focus – high power focus
Light source
Base - support

8. Label the parts on your microscope picture.
Eyepiece/ocular lens magnification = 10X
Arm-Support
Stage - support
Coarse adjust – general focus
Diaphragm – adjusts amount of light
Fine Focus – high power focus
Light source
Base - support

9. Label the parts on your microscope picture.
Eyepiece/ocular lens magnification = 10X
Arm-Support
Stage - support
Stage clips – holds slide
Coarse adjust – general focus
Diaphragm – adjusts amount of light
Fine Focus – high power focus
Light source
Base - support

10. Label the parts on your microscope picture.
Eyepiece/ocular lens magnification = 10X
Arm-Support
Objective Lenses Scanning = 4X
Low Power = 10X
High power = 40X
Stage - support
Stage clips – holds slide
Coarse adjust – general focus
Diaphragm – adjusts amount of light
Fine Focus – high power focus
Light source
Base - support

11. Label the parts on your microscope picture.
Eyepiece/ocular lens magnification = 10X
Revolving nosepiece
Arm-Support
Objective Lenses Scanning = 4X
Low Power = 10X
High power = 40X
Stage - support
Stage clips – holds slide
Coarse adjust – general focus
Diaphragm – adjusts amount of light
Fine Focus – high power focus
Light source
Base - support

12. Label the parts on your microscope picture.
Eyepiece/ocular lens magnification = 10X
Revolving nosepiece
Arm-Support
Objective Lenses Scanning = 4X
Low Power = 10X
High power = 40X
Stage - support
Stage clips – holds slide
Coarse adjust – general focus
Diaphragm – adjusts amount of light
Fine Focus – high power focus
Light source
Base - support

13. Magnification of a Compound Microscope
Because you are looking through multiple lenses the lenses have a “compounding” effect.
The eyepiece always magnifies 10X
Eyepiece
Objective Lens
Magnification
Scanning lens (red)
10X
Low Power (yellow)
High Power (blue)

14. Magnification of a Compound Microscope
Each of the objective lenses have their own magnification
Eyepiece
Objective Lens
Magnification
Scanning lens (red)
10X 4X
Low Power (yellow)
High Power (blue)
  40X

15. Magnification of a Compound Microscope
You then multiply the eyepiece with the objective lens to determine the total magnification
Eyepiece
Objective Lens
Magnification
Scanning lens (red)
10X 4X
Low Power (yellow)
100X
High Power (blue)
40X
400X
40X

16. General Procedures
·  Make sure all backpacks are out of the aisles before you get a microscope! Always carry the microscope with one hand on the Arm and one hand on the Base.
·  Wear your glasses, the microscope will focus to your eyesight!
·  Keep both eyes open, your brain will learn to ignore the other eye.

17. Focusing a Specimen
Always start on low power or scanning.

18. Focusing a Specimen Always start on low power or scanning.
Focus the specimen using the course adjust.

19. Focusing a Specimen Always start on low power or scanning.
Focus the specimen using the course adjust.
Re-center. If high power is needed, turn only after re-centering!

20. Focusing a Specimen Always start on low power or scanning.
Focus the specimen using the course adjust.
Re-center. If high power is needed, turn only after re-centering!
Now use the FINE FOCUS ONLY for your final adjustments.

21. Care of microscopes
A. You must always keep the microscope clean and dry. B. When a slide is focused under the high power objective, it should not be pulled or removed. C. The microscope lenses must be cleaned by lens paper after each time using the microscope. Xylene can be used as a solvent for erasing the dusts of the lenses.

22. Care of microscopes
D. When you are going to view specimens first view under the low power objective lens, then bring the specimen into the focus by coarse adjustment screw. After that if you want to get a more magnified view then rotate the nose piece to the high power objective lens and then bring the specimen into focus by using the fine adjustment screw. E. Avoid lowering the objective lens into the slide, especially if there is not a coverslip. Even when using a coverslip, be careful and mindful of you raising the slide to far up and crushing the coverslip and damaging both the slide and microscope.

23. Care of microscopes
F. Never remove the slide from the stage when it is focused under the high power objective. G. When the use is over, clean all lenses with lens paper and carefully (with two hands) replace microscope on shelf.

24. Care of microscopes Always carry microscope with two hands.
Store microscope with scan strength (lowest power) objective in place.