1. ENERGY SOURCES FOR MUSCLE CONTRACTION

2. Objectives 1.Energy used 2.Energy produced 3.Oxygen debt 4.Muscle fiber types 5.Muscle fatigue

4. ATP Muscle cells convert chemical energy to mechanical energy. ATP is the energy source used for this conversion. The ATP pool in skeletal muscle is small and capable of supporting only a few contractions if not replenished.

5. Creatine Phosphate Immediate high-energy source for replenishing the ATP supply The enzyme creatine phosphokinase (CPK) catalyzes the reaction

9. Carbohydrates Muscle cells contain glycogen, Glycogen provide glucose for oxidative phosphorylation and glycolysis

10. Fatty Acids and Triglycerides Fatty acids represent an important source of energy for muscle cells during prolonged exercise. Muscle cells contain fatty acids but can also take up fatty acids from blood. In addition, muscle cells can store triglycerides, which can be hydrolyzed when needed to produce fatty acids

11. Metabolic Adaptations Allow Contraction to Continue With an Inadequate Oxygen Supply Glycolytic (anaerobic) metabolism can provide energy for sudden, rapid, and forceful contractions of some muscles. In most muscles, especially under conditions of rest or moderate exercise, the supply of oxygen is adequate for aerobic metabolism (fed by fatty acids and by the end products of glycolysis) to supply the energy needs of the contractile system.

12. Muscle tissue has two sources of oxygen (1) oxygen that diffuses into muscle fibers from the blood and (2) oxygen released by myoglobin within muscle fibers.

13. Depletion of aerobic energy capacity This results from two effects: (1) the oxygen debt and (2) depletion of the glycogen stores of the muscles

14. Oxygen debt The body normally contains about 2 liters of stored oxygen that can be used for aerobic metabolism even without breathing any new oxygen. This stored oxygen consists of the following: (1) 0.5 liter in the air of the lungs, (2) 0.25 liter dissolved in the body fluids, (3) 1 liter combined with the hemoglobin of the blood, and (4) 0.3 liter stored in the muscle fibers them-selves, combined mainly with myoglobin

15. After the exercise is over, this stored oxygen must be replenished by breathing extra amounts of oxygen over and above the normal requirements. In addition, about 9 liters more oxygen must be consumed to provide for reconstituting both the phosphagen system and the lactic acid system. All this extra oxygen that must be “repaid,” about 11.5 liters, is called the oxygen debt.

17. Measuring Maximal Oxygen Uptake Is the most Common Method of Quantifying Dynamic Exercise

18. Oxygen debt If the energy demands of exercise cannot be met by oxidative phosphorylation, an oxygen debt is incurred. After completion of exercise, respiration remains above the resting level in order to "repay" this oxygen debt. The extra oxygen consumption during this recovery phase is used to restore metabolite levels (such as creatine phosphate and ATP) and to metabolize the lactate generated by glycolysis. The increased cardiac and respiratory work during recovery also contributes to the increased oxygen consumption seen at this time and explains why more O 2 has to be "repaid" than was "borrowed."

20. TYPES OF SKELETAL MUSCLE FIBERS

21. Slow oxidative fibers Fast glycolytic fibers Myoglobin content LargeSmall MitochondriaManyFew CapillariesManyFew ColourRedWhite (Pale) Capacity for generating ATP and method used High capacity, aerobic respiration Low capacity, anaerobic respiration Glycogen stores LowHigh Creatine Kinase Lowest amountHighest amount Contraction velocity SlowFast Fatigue resistance HighLow Location where fibers are abundant Postural muscles such as those of the neck Upper limb muscles Primary function of the fibers Maintaining posture and aerobic endurance activities Rapid intense movements of short duration

22. Classification of Fiber Types in Skeletal Muscles

23. Examples: Type I Red fibers: in postural muscles – Large myoglobin content and many mitochondria Type IIa Red fibers: in muscles needed for activities like middle distance running, swimming, etc. – Large myoglobin content and many mitochondria Type IIb White fibers: needed for activities like sprinting Low myoglobin content and few mitochondria

24. Muscle fatigue. The inability of a muscle to maintain force of contraction after prolonged activity. Causes-  inadequate release of calcium ions from the SR.  Depletion of creatine phosphate  insufficient oxygen,  depletion of glycogen and other nutrients,  build up of lactic acid and ADP,  failure of action potentials in the motor neuron to release enough acetylcholine.

26. Q. Fast twitch muscle fiber differ from slow twitch muscles in that former: a.have low ATPase activity b.are adapted for posture maintaining contraction c.contains more sarcoplasmic reticulum with high glycogen content d.resistant to fatigue