1. Process of Genetic Modification “Genetic engineering is just an extension of natural processes”

2. What is canola oil? What is a canola? Also known as rapeseed Stands for CANadian Oil Low Acid

3. How are GMOs produced? Strawberries that express a gene from an arctic fish? Why might this be desirable?

4. How are GMOs made?

5. The general idea is simple.. (3 components you need) The gene you want to transfer The target species A Vector

6. How can a gene be excised? restriction endonucleases

7. Outcomes of Genetic Modification “Genetic engineering will deliver more nutritious crops and increase yield potential”

8. Herbicide Resistance Selective vs. non- selective herbicides Spray less herbicides Roundup ready soybeans

9. Pest Resistant Bt (Bacillus thuringiensis) crops Produce their own insecticide Reduce need for sprayed pesticides

10. Nutrition Golden Rice Potatoes Coffee

11. Atlantic Salmon Genes from other fish species to produce growth hormones and allow for growth year round, even in colder water Fish reach market size in 18 months, compared with 3 years

12. Safety of GMOs “Genetically engineered products are completely safe for human use/consumption”

13. Plant microRNAs in Your Blood miRNA passed from the food through digestion into your blood and attach onto your organs to regulate functions. examples: Exogenous plant MIR 168a This miRNA inhibits a protein that remove LDL cholesterol from the blood.

14. Bt corn Bt corn pollen’s effects on non-target organisms Studies on monarch larvae who feed on milkweed leaves dusted with Bt corn pollen

15. Bt corn-StarLink corn StarLink corn contains Cry9C, incorporated from Bt sp. – Stock Feed – Taco Bell – Further detection

16. Bt-Toxin in Your Blood! Sherbrooke University Hospital in Quebec Bt-toxin in the blood of: – 93 % of pregnant women tested – 80 % of umbilical blood in their babies – 67 % of non-pregnant women  Food sources: animals fed Bt corn, processed food and drink of high fructose corn syrup…  Scientists speculate Bt toxin can cause autoimmune diseases, food allergies, gastrointestinal problems, child learning disorders… (more tests are needed!)

17. Effects of GMO/Roundup Food

18. Detect GMOs 1.Herbicide bioassays 2.ELISA (“dipstick”, “plate” tests) 3. PCR (polymerase chain reaction)

19. Future Applications “Genetic engineering is only used to improve food products”

20. Pharming Farming + Pharmaceuticals – Bacteria – Plants – Animals

21. Products of Pharming Insulin (bacteria) Various Proteins (PMPs) – Antibodies, antigens, enzymes, hormones, structural proteins, Atryn (goats)

22. Insects Mosquitoes – Dengue Fever Pink Bollworms – Cotton Crops

23. GloFish Pets Research