1. Electron Microscopy 1 Electron Microscopy (EM) Applying Atomic Structure Knowledge to Chemical Analysis

2. Electron Microscopy 2 Electron Microscopes achieve higher magnification and resolution than optical scopes. use electrons instead of light to image samples. use electromagnets (instead of glass lenses) to focus the electron beams. require samples to be exposed to the electron beam in a vacuum. allow for analysis of a sample’s composition in addition to imaging.

3. Electron Microscopy 3 Electron Microscopes Scanning Electron Microscopes: Scan (raster) across a sample surface to get a high resolution images. used for many different types of samples. Transmission Electron Microscopes: creates an image after passing electrons through an ultra thin sample that is somewhat optically transparent. are used for biological and metrology samples to get high resolution images of cells, crystals, and their structures.

4. Electron Microscopy 4 Electronics and Signal Processing Video Signals Computer E-Beam, Magnet, and Detector Control Systems Video Signal Electro-Optical Column Sample Chamber Scanning Electron Microscope Instrument Diagram

5. Electron Microscopy 5 Scanning Electron Microscope Hitachi S800 SEM at the USF College of Engineering Metrology Laboratory Image and x-ray analysis and processing Sample compartment Electron beam Control system for e-beam, magnetic fields, and communications

6. Electron Microscopy 6 SEM Electron Beam

7. Electron Microscopy 7 SEM of a filter paper with 0.45 micron (0.45 x 10 -6 meters) pores at 1000x magnification. The white particles are aluminum oxide. SEM Image of Filter Paper

8. Electron Microscopy 8 SEM of the same filter paper with 0.45 micron (0.45 x 10 -6 meters) pores at 10,000x magnification. The white particles are aluminum oxide. SEM Image of Filter Paper

9. Electron Microscopy 9 Sample SEM Images SEM of the old USF logo etched on a polymer substrate. Note the micron scale.

10. Electron Microscopy 10 Sample SEM Images SEM of nickel (white) film test pattern on silicon carbide substrate. Note the 100 micron scale.

11. Electron Microscopy 11 Medical Applications: compare healthy and unhealthy blood and tissue samples study the effects of medication on patients analysis of crime scene evidence Structural Applications: faults and weaknesses of building materials crystal structure of materials characterization of size, shape, and distribution of various particulates (such as air pollution) adsorption of particles on surfaces Electron Microscope Applications

12. Electron Microscopy 12