1. Microscopy Looking at the small stuff

2. Microscopes  “micro” means : Small  “scope” means: Watch/ look  It was actually developed first as entertainment for the rich

3. Light Microscope  Focuses visible light rays  Can be used on live or dead specimens  Dyes and fluorescent markers help for viewing  Magnify up to 1000X on the ones we have  Examples: Compound light microscope Confocal microscope

4. Light Microscope

5. Light Micrographs Plant magnified Human chromosomes 

6. Confocal Microscope

7. Confocal micrograph Spirogyra crassa Maximum projection green: Actin. Rhodamin, Label: Phalloidin. Actin red: Chloroplasts. Autofluorescence Image acquired with the Leica TCS SP2

8. Electron Microscopy (EM)  Focus beam of electrons on the specimen  Specimens must be dead  Kinds Transmission Electron Microscope (TEM)  Cross-section of the material  Most detailed of internal of cell Scanning Electron Microscope (SEM)  Surface of the specimen  Shows texture, but less detail

9. TEM

11. SEM

12. Ant Pollen Grains

13. Red Blood Cells

14. Compare cardiac myocytes Maximum projection (overlay) of neonatal cardiac myocytes from rat. green: Myomesin, FITC red: Cadherin, Cy3 blue: DNA (nuclei), DRAQ5 Image acquired with the Leica TCS SL Compare to TEM of myoctes on the wall.

15. Keen eyes  Light microscopes will not show you the nucleus of a cell or something the size of a virus!  Confocal microscopy uses brightly colored markers  TEM is detailed, flat, and black and white  SEM is 3D, textured, and black and white

16. Cited pictures  Pollen grains (1000x magnification). Paul Fuqua. 2003. unitedstreaming. 29 August 2006 http://www.unitedstreaming.com/ http://www.unitedstreaming.com/  Blood cells, red; microscopic view. U.S. Army. 2006. unitedstreaming. 29 August 2006 http://www.unitedstreaming.com/ http://www.unitedstreaming.com/  http://www- medlib.med.utah.edu/WebPath/HEMEHTML/HEME001.h tml http://www- medlib.med.utah.edu/WebPath/HEMEHTML/HEME001.h tml  www.plymouth.ac.uk/upload/SCI/liverartery.jpg www.plymouth.ac.uk/upload/SCI/liverartery.jpg  http://www.confocal- microscopy.com/WebSite/pictures.nsf/(ALLIDs)/23C65 31FD6B41E6BC1256A7D0053F8CA/$FILE/drosoegg_ap p_img_i_cl.jpg http://www.confocal- microscopy.com/WebSite/pictures.nsf/(ALLIDs)/23C65 31FD6B41E6BC1256A7D0053F8CA/$FILE/drosoegg_ap p_img_i_cl.jpg  http://www.confocal- microscopy.com/WebSite/pictures.nsf/(ALLIDs)/23C65 31FD6B41E6BC1256A7D0053F8CA/$FILE/drosoegg_ap p_img_i_cl.jpg

17. Cited Pictures  http://remf.dartmouth.edu/images/insectPart3SE M/image/31formicidae40(extra).jpg http://remf.dartmouth.edu/images/insectPart3SE M/image/31formicidae40(extra).jpg  http://mikes- machine.mine.nu/Mysterymoss/Micro3_DSCN648 6.jpg http://mikes- machine.mine.nu/Mysterymoss/Micro3_DSCN648 6.jpg  http://www.nims.go.jp/analysis/TEM.jpg http://www.nims.go.jp/analysis/TEM.jpg  http://www.nims.go.jp/analysis/SEM.jpg http://www.nims.go.jp/analysis/SEM.jpg  http://cdr.rfmh.org/NewFiles/Components/images /confocal%20microscope.jpg http://cdr.rfmh.org/NewFiles/Components/images /confocal%20microscope.jpg  http://131.229.114.77/microscopy/images/chrom osomes2.jpg http://131.229.114.77/microscopy/images/chrom osomes2.jpg  http://www.biosci.ohio- state.edu/~plantbio/osu_pcmb/images/Courses/P B101/plant_cell_tem.jpg