2. Compound Microscope

3. How to properly carry the microscope Two-dimensional images View internal structures

4. With a binocular microscope, adjust oculars for both eyes!

5. Stereoscopic (Dissecting) Microscope Three-dimensional images View external structures

6. Stereoscopic close-up of finger

7. Microsurgery being performed with a stereoscope

8. Fluorescence Microscopy  Cells are stained with fluorescent dyes called fluorochromes.  Uses UV light as energy source.  Fluorochromes absorb UV light and emit visible light.  Produces green to yellow color

9. Digital Microscope System Automated system: Auto-focus, auto-aperture, auto- light……

10. Electron Microscopy  Uses a beam of electrons  Very high magnification of up to 100,000 X.  Transmission electron microscope (TEM): Two-dimensional images Two-dimensional images View internal structures View internal structures  Scanning electron microscope (SEM): Three-dimensional images Three-dimensional images View external structures View external structures

11. TEM and SEM Compared Scanning Electron Microscope Transmission Electron Microscope

13. Transmission Electron Microscopy (a) a virus and (b) a protozoan. E. coli

14. Scanning Electron Microscopy ParameciumAspergillus