1. 0 100 200 Condensed TUNEL + cells (%) WT RanBP9 Tg B The scale bar represents 50  m A CA1 TUNEL Wild TypeRanBP9 Tg P<0.0005 * Supplemental Fig. S1

2. Supplemental Fig. S2 A B C D

3. Supplemental Fig. S3

4. Supplemental Fig. S4

5. Thy1.2 pEYFP-N1 GFP- RanBP9 VC RanBP9 O.D at 490/690 nm VC RanBP9 pEYFP-N1 GFP RanBP9 B C UL: 2.42 UR: 28.40 LL: 47.36 LR: 21.82 UL: 0.68 UR: 24.56 LL: 29.62 LR: 45.14 RanBP9 21.87%45.14% A VC PI Annexin V Supplemental Fig. S5 50 100 75 37 25 20 10 15 150 250 Stacking gel 6E10 O F 4 tetramer trimer dimer monomer fibril D

6. HT22 Cells Cont. RanBP9 siRNA * RanBP9 * β-actin Supplemental Fig. S6 AB * Cofilin * β-actin Cont. Cofilin siRNA C Control DAPT 5μM APP-FL APP-CTFs