1. Chapter 9 Photosynthesis “Sunshine splits apart the carbon dioxide that a plant has absorbed from the air, the plant throws out at that time the oxygen alone and keeps the carbon to itself as nourishment.” — Jan Ingenhousz (1779)

2. Autotrophs and Heterotrophs  Autotrophs are organisms that make their own food.  They obtain everything they need by using CO 2 and inorganic compounds from the environment.  Heterotrophs are unable to make their own food and survive on compounds made by other organisms.  Plants are autotrophic and animals are heterotrophic.

3. Chloroplasts  Chloroplasts are the main site of photosynthesis in plants.  They are found in all green parts of plants.  Chlorophyll is the green pigment found in chloroplasts and is responsible for a plant’s color.  They absorb light and drive the synthesis of organic molecules.

4. Chloroplasts  CO 2 and O 2 enter and exit the leaf through pores called stomata.  The leaves contain veins that deliver water to the leaves and sugar to the roots of the plant.

5. Chloroplasts  The chloroplast is enclosed by 2 membranes. Within the chloroplast is the stroma, the site of the Calvin-Benson cycle (“dark reactions”, or the light independent reactions).

6. Chloroplasts  Tiny interconnected stacks of thylakoids are called grana. Inside of the thylakoids, the thylakoid space is where the “light” reactions of photosynthesis take place.

7. Photosynthesis  In general, photosynthesis is carried out according to the following equation:  6CO 2 + 12H 2 O + light energy --> C 6 H 12 O 6 + 6O 2 + 6H 2 O  Which is essentially the reverse of cellular respiration:  C 6 H 12 O 6 + 6O 2 --> 6CO 2 + 6H 2 O + energy

9. Photosynthesis: The Light Reactions  The light reactions convert solar energy into chemical energy.  When light is absorbed by the chlorophyll. Water is split, O 2 is given off, electrons and hydrogen atoms get transferred from water to NADP + making NADPH.  The light reactions also generate ATP from ADP.  This all occurs in the thylakoids.

10. Photosystems I & II

11. Photosynthesis: The Calvin Cycle, a.k.a. The Dark Reactions  The Calvin cycle incorporates CO 2 from the air into organic molecules present in the chloroplast via a process called carbon fixation.  Here, NADPH and ATP (from the light reactions) convert CO 2 into carbohydrate.  They occur in the stroma.

13. The Electromagnetic Spectrum  The sun radiates energy that makes up the full electromagnetic spectrum from radio waves to  -rays.  Our atmosphere filters out all but a small portion of the radiation, and the energy that gets through (the visible spectrum for the most part) is what drives photosynthesis.  The visible spectrum is ~400-700nm.

15. Light  When light hits matter, 3 basic things happen:  1. It is reflected.  2. It is transmitted.  3. It is absorbed.

16. Light  When white light hits an object, only the color that is reflected is the one we see, all others are absorbed.  For example, leaves are green, because chlorophyll doesn’t absorb green light. It reflects it, and absorbs all other colors.

17. The 3 Main Pigments in Chloroplasts  1. Chlorophyll a- which absorbs violet- blue and red light the best.  2. Chlorophyll b- which absorbs violet- blue and red-orange light the best.  3. Carotenoids absorb violet and blue-green light the best.

18. Chlorophyll a and Accessory Pigments  Chlorophyll a is the main photosynthetic pigment.  Chlorophyll b and the carotenoids are accessory pigments.

19. Chlorophyll a and Accessory Pigments  Chlorophyll b and the carotenoids help to broaden the spectrum of useful photosynthetic pigments.  Carotenoids also have an important function in photoprotection--that is, they absorb some of the light energy and dissipate the heat that would otherwise damage the plant.

22. Comparing Oxygen Production and Absorption of Light in Photosynthesis  If you notice, the points at which the O 2 production is the greatest overlaps with the points at which light absorption is the greatest.

23.  Theodore Englemann  Experimented with aerophilic bacteria and long spiral algae called spirogyra.  Used light to illuminate the specimens after it had been passed through a prism.  Found that bacteria congregated in areas of violet-blue and red light.

24. Photosystems  In the thylakoid membrane, chlorophyll molecules are organized with proteins into what are known as photosystems II and I.

25. 25 Photosystems  These are essentially reaction centers surrounded by light-harvesting complexes which consist of chlorophyll a/b and/or carotenoids bound to proteins.

26. Photosystems II and I  There are two types of photosystems that exist in the thylakoid membrane, photosystem II and photosystem I. They function sequentially and are named in the order of their discovery.  Photosystem II actually occurs 1st.

27. Photosystems II and I  The pigment molecules that make up the two photosystems are actually the same, but the difference is the proteins with which they are associated. It makes the difference in the wavelength of light they absorb.  These two photosystems work together to make ATP and NADPH.

30. The Light Reactions and The Electron Transport Chain  The light reactions and electron flow The light reactions and electron flow

31.  Light reactions occur in the thylakoid space  The Calvin cycle occurs in the stroma

32. The Calvin Cycle  The Calvin cycle The Calvin cycle