1. Midterm Review Jeopardy $100 $200 $300 $400 $500 $100 $200 $200 $300 $400 $500 ControlMetabolism Gene Expression Cells Microbial Growth Chemistry $100 $200 $300 $400 $500 $100 $200 $300 $400 $500

2. Chapter 5: Control: $100 Question Which of these control methods destroys DNA? a. Alcohols. b. Washing. c. UV light. d. Silver Nitrate. BACK TO GAME ANSWER

3. Chapter 5: Control: $100 Answer BACK TO GAME Which of these control methods destroys DNA? a. Alcohols. b. Washing. c. UV light. d. Silver Nitrate.

4. Chapter 5: Control: $200 Question Chlorine bleach: a. Becomes inactivated by large quantities of organic compounds b. Is unable to destroy endospores c. Is ineffective when it is diluted d. Is expensive relative to other chemical control methods BACK TO GAME ANSWER

5. Chapter 5: Control: $200 Answer BACK TO GAME Chlorine bleach: a. Becomes inactivated by large quantities of organic compounds b. Is unable to destroy endospores c. Is ineffective when it is diluted d. Is expensive relative to other chemical control methods

6. Chapter 5: Control: $300 Question Typical conditions used for sterilization in an autoclave are: a. 100°C for 10 minutes. b. 121°C at 15 psi for 15 minutes. c. 80°C for 15 minutes. d. 72°C for 15 seconds. BACK TO GAME ANSWER

7. Chapter 5: Control : $300 Answer BACK TO GAME Typical conditions used for sterilization in an autoclave are: a. 100°C for 10 minutes. b. 121°C at 15 psi for 15 minutes. c. 80°C for 15 minutes. d. 72°C for 15 seconds.

8. Chapter 5: Control : $400 Question Preserving meat with salt kills microbes by: a. Causing plasmolysis b. Lysing cells c. Preventing protein synthesis d. Oxidizing DNA BACK TO GAME ANSWER

9. Chapter 5: Control : $400 Answer BACK TO GAME Preserving meat with salt kills microbes by: a. Causing plasmolysis b. Lysing cells c. Preventing protein synthesis d. Oxidizing DNA

10. Chapter 5: Control : $500 Question Which type of electromagnetic radiation has the most energy? a. X rays b. Gamma rays c. UV rays d. Microwaves BACK TO GAME ANSWER

11. Chapter 5: Control : $500 Answer BACK TO GAME Which type of electromagnetic radiation has the most energy? a. X rays b. Gamma rays c. UV rays d. Microwaves

12. Chapter 6: Metabolism: $100 Question Which of these would be an anabolic process? a.Glycolysis. b.Fermentation. c. DNA replication. d. Digestion. BACK TO GAME ANSWER

13. Chapter 6: Metabolism: $100 Answer BACK TO GAME Which of these would be an anabolic process? a.Glycolysis. b.Fermentation. c. DNA replication. d. Digestion.

14. Chapter 6: Metabolism: $200 Question In this reaction, tell whether NAD + is oxidized or reduced. NAD + + Pyruvate  NADH + Lactate BACK TO GAME ANSWER

15. Chapter 6: Metabolism: $200 Answer NAD + is getting reduced. BACK TO GAME

16. Chapter 6: Metabolism: $300 Question Which of these processes generates the most reducing power? a. Glycolysis b. Intermediate step c. Pentose Phosphate pathway d. TCA cycle BACK TO GAME ANSWER

17. Chapter 6: Metabolism: $300 Answer BACK TO GAME Which of these processes generates the most reducing power? a. Glycolysis b. Intermediate step c. Pentose Phosphate pathway d. TCA cycle

18. Chapter 6: Metabolism: $400 Question Which of these would generate the most ATP? a. Aerobic cellular respiration in a prokaryote b. Aerobic cellular respiration in a eukaryote c. Anaerobic cellular respiration in a prokaryote d. Fermentation BACK TO GAME ANSWER

19. Chapter 6: Metabolism: $400 Answer BACK TO GAME Which of these would generate the most ATP? a. Aerobic cellular respiration in a prokaryote b. Aerobic cellular respiration in a eukaryote c. Anaerobic cellular respiration in a prokaryote d. Fermentation

20. Chapter 6: Metabolism: $500 Question Which of these statements about photosynthesis is FALSE? a. The light reactions produce ATP and NADPH b. The Calvin Cycle produces G3P, which can be used to synthesize fructose c. After the reaction center chlorophyll in Photosystem I loses electrons, it splits water to get new electrons d. When light hits chlorophyll, it causes electrons to become excited and then fall back to ground state, releasing energy BACK TO GAME ANSWER

21. Chapter 6: Metabolism: $500 Answer BACK TO GAME Which of these statements about photosynthesis is FALSE? a. The light reactions produce ATP and NADPH b. The Calvin Cycle produces G3P, which can be used to synthesize fructose c. After the reaction center chlorophyll in Photosystem I loses electrons, it splits water to get new electrons d. When light hits chlorophyll, it causes electrons to become excited and then fall back to ground state, releasing energy

22. Chapter 7: Gene Expression: $100 Question Which of these is NOT a difference between DNA and RNA? a. DNA has T, RNA has U b. DNA is made of nucleotides with 3 phosphates, RNA nucleotides have 1 phosphate. c. DNA usually has 2 strands, RNA has 1. d. They have different sugars. BACK TO GAME ANSWER

23. Chapter 7: Gene Expression: $100 Answer BACK TO GAME Which of these is NOT a difference between DNA and RNA? a. DNA has T, RNA has U b. DNA is made of nucleotides with 3 phosphates, RNA nucleotides have 1 phosphate. c. DNA usually has 2 strands, RNA has 1. d. They have different sugars.

24. Chapter 7: Gene Expression: $200 Question Which of these would only be found in eukaryotes? a. Repressors and activators b. Polycistronic transcripts c. 5’ caps and poly A tails d. Operons BACK TO GAME ANSWER

25. Chapter 7: Gene Expression: $200 Answer BACK TO GAME Which of these would only be found in eukaryotes? a. Repressors and activators b. Polycistronic transcripts c. 5’ caps and poly A tails d. Operons

26. Chapter 7: Gene Expression : $300 Question The mRNA transcript reads: 5’ AUGUAUAACGAG 3’ What would the – strand of DNA read? a. 5’ ATGTATAACGAG 3’ b. 3’ TACATATTGCTC 5’ c. 5’ TACATATTGCTC 3’ d. 3’ UACAUAUUGCUC 5’ BACK TO GAME ANSWER

27. Chapter 7: Gene Expression : $300 Answer BACK TO GAME The mRNA transcript reads: 5’ AUGUAUAACGAG 3’ What would the – strand of DNA read? a. 5’ ATGTATAACGAG 3’ b. 3’ TACATATTGCTC 5’ c. 5’ TACATATTGCTC 3’ d. 3’ UACAUAUUGCUC 5’

28. Chapter 7: Gene Expression: $400 Question In order for the lac operon to be transcribed, what has to happen? a. Lactose levels must be low and glucose must be high b. Glucose induces an activator and lactose bonds to a phosphate group c. Lactose removes a repressor and cAMP induces an activator d. cAMP removes a repressor and glucose removes an activator BACK TO GAME ANSWER

29. Chapter 7: Gene Expression: $400 Answer BACK TO GAME In order for the lac operon to be transcribed, what has to happen? a. Lactose levels must be low and glucose must be high b. Glucose induces an activator and lactose bonds to a phosphate group c. Lactose removes a repressor and cAMP induces an activator d. cAMP removes a repressor and glucose removes an activator

30. Chapter 7: Gene Expression: $500 Question During quorum sensing, after signal molecules reach a high concentration, what is likely to happen in the cell? a. The cell is prevented from synthesizing new cell walls b. The cell attaches to a phosphate group and is activated to do work c. Signal molecules diffuse into the cell and cause mutations in the DNA d. Signal molecules attach to a sensor, which phosphorylates a response regulator to turn on genes BACK TO GAME ANSWER

31. Chapter 7: Gene Expression: $500 Answer BACK TO GAME During quorum sensing, after signal molecules reach a high concentration, what is likely to happen in the cell? a. The cell is prevented from synthesizing new cell walls b. The cell attaches to a phosphate group and is activated to do work c. Signal molecules diffuse into the cell and cause mutations in the DNA d. Signal molecules attach to a sensor, which phosphorylates a response regulator to turn on genes

32. Chapter 4: Microbial Growth: $100 Question An agar plate is an example of: a. Continous culture b. A chemostat c. Closed culture d. Liquid medium BACK TO GAME ANSWER

33. Chapter 4: Microbial Growth: $100 Answer BACK TO GAME An agar plate is an example of: a. Continous culture b. A chemostat c. Closed culture d. Liquid medium

34. Chapter 4: Microbial Growth: $200 Question On an agar plate, which part of a colony will be in the latest stage of the growth curve? a. The center b. The edges c. All areas of the colony are in the same stage of the growth curve BACK TO GAME ANSWER

35. Chapter 4: Microbial Growth: $200 Answer BACK TO GAME On an agar plate, which part of a colony will be in the latest stage of the growth curve? a. The center b. The edges c. All areas of the colony are in the same stage of the growth curve

36. Chapter 4: Microbial Growth: $300 Question Bacteria that prefer to live in oxygen but can live in environments with or without oxygen are called: a. Aerotolerant anaerobes b. Microaerophiles c. Obligate anaerobes d. Facultative anaerobes BACK TO GAME ANSWER

37. Chapter 4: Microbial Growth: $300 Answer BACK TO GAME Bacteria that prefer to live in oxygen but can live in environments with or without oxygen are called: a. Aerotolerant anaerobes b. Microaerophiles c. Obligate anaerobes d. Facultative anaerobes

38. Chapter 4: Microbial Growth: $400 Question A type of agar that included specific growth factors to favor the growth of one species of microbe above all others would be: a. selective b. differential c. enrichment d. chemically defined BACK TO GAME ANSWER

39. Chapter 4: Microbial Growth: $400 Answer BACK TO GAME A type of agar that included specific growth factors to favor the growth of one species of microbe above all others would be: a. selective b. differential c. enrichment d. chemically defined

40. Chapter 4: Microbial Growth: $500 Question This type of organism can fix its own carbon and obtains energy from chemicals found in its environment: a. photoautotroph b. photoheterotroph c. chemoautotroph d. chemoheterotroph BACK TO GAME ANSWER

41. Chapter 4: Microbial Growth: $500 Answer BACK TO GAME This type of organism can fix its own carbon and obtains energy from chemicals found in its environment: a. photoautotroph b. photoheterotroph c. chemoautotroph d. chemoheterotroph

42. Chapter 2: Chemistry: $100 Question BACK TO GAME ANSWER

43. Chapter 2: Chemistry: $100 Answer BACK TO GAME

44. Chapter 2: Chemistry: $200 Question Which of these is NOT a type of lipid? a. phospholipid b. polypeptide c. steroid d. triglyceride BACK TO GAME ANSWER

45. Chapter 2: Chemistry: $200 Answer BACK TO GAME Which of these is NOT a type of lipid? a. phospholipid b. polypeptide c. steroid d. triglyceride

46. Chapter 2: Chemistry: $300 Question A basic solution has a _____ concentration of H+ than OH- and a pH _______ than 7. a. greater, greater b. lower, lower c. greater, lower d. lower, greater BACK TO GAME ANSWER

47. Chapter 2: Chemistry: $300 Answer BACK TO GAME A basic solution has a _____ concentration of H+ than OH- and a pH _______ than 7. a. greater, greater b. lower, lower c. greater, lower d. lower, greater

48. Chapter 2: Chemistry: $400 Question These structures are 3 different: a. isomers b. isotopes c. glycerols d. fatty acids BACK TO GAME ANSWER

49. Chapter 2: Chemistry: $400 Answer BACK TO GAME These structures are 3 different: a. isomers b. isotopes c. glycerols d. fatty acids

50. Chapter 2: Chemistry: $500 Question Name 3 major parts of ATP. BACK TO GAME ANSWER

51. Chapter 2: Chemistry: $500 Answer BACK TO GAME Name 3 major parts of ATP. A ribose sugar, adenine base, and 3 phosphate groups.

52. Chapter 3: Cells: $100 Question If you wanted to see individual small molecules, which microscope should you use? a. bright field b. Dark field c. Scanning electron d. Atomic force BACK TO GAME ANSWER

53. Chapter 3: Cells : $100 Answer BACK TO GAME If you wanted to see individual small molecules, which microscope should you use? a. bright field b. Dark field c. Scanning electron d. Atomic force

54. Chapter 3: Cells: $200 Question What happens to a cell placed in a hypertonic solution? a. It swells b. It shrinks c. It stays the same size BACK TO GAME ANSWER

55. Chapter 3: Cells : $200 Answer BACK TO GAME What happens to a cell placed in a hypertonic solution? a. It swells b. It shrinks c. It stays the same size

56. Chapter 3: Cells: $300 Question In which condition would you expect endospores to form? a. Bacteria have just been streaked onto new agar b. Bacteria have just been placed in the incubator c. Bacteria have been living on the same agar for 2 weeks d. Bacteria are living in a biofilm in their natural habitat BACK TO GAME ANSWER

57. Chapter 3: Cells : $300 Answer BACK TO GAME In which condition would you expect endospores to form? a. Bacteria have just been streaked onto new agar b. Bacteria have just been placed in the incubator c. Bacteria have been living on the same agar for 2 weeks d. Bacteria are living in a biofilm in their natural habitat

58. Chapter 3: Cells: $400 Question Lysozyme targets _____ bacteria by _______ cell walls. a. Gram positive, inhibiting synthesis of b. Gram negative, inhibiting synthesis of c. Gram positive, breaking down d. Gram negative, breaking down BACK TO GAME ANSWER

59. Chapter 3: Cells : $400 Answer BACK TO GAME Lysozyme targets _____ bacteria by _______ cell walls. a. Gram positive, inhibiting synthesis of b. Gram negative, inhibiting synthesis of c. Gram positive, breaking down d. Gram negative, breaking down

60. Chapter 3: Cells: $500 Question What is a plasmid and why are they important? BACK TO GAME ANSWER

61. Chapter 3: Cells : $500 Answer BACK TO GAME What is a plasmid and why are they important? A small, round piece of DNA that can be transferred between bacteria, allowing them to share traits such as antibiotic resistance.