1. A Tour of the Cell

2. Discovery of cells needed microscopes 1665 – Robert Hooke observes “boxes” in cork bark; he calls them cells. 1674 – Anton van Leeuwenhoek observes single celled organisms in well water

4. Three types of microscope images (Figure 4.1)

5. Cell theory 1.Schwann and Schleiden (1839) - cells are the elementary unit of all plants and animals 2.Virchow (1858)- cells come from preexisting cells 3.Modern cell theory states that cells carry out the biochemical processes of life. 4.Viruses do not fit into the cell theory and are not considered living

6. Relative Sizes (Fig. 4.3)

7. Cells are small 1-100 µm (1µm = 0.001 mm) Cells must exchange materials with their environment through their membrane. Cells need high surface area to volume ratio in order to maximize this exchange.

8. As size increases, surface area to volume ratio decreases LengthSA = 6 * length 2 V = Length 3 SA/V 1616 22483 354272 496641.5 51501251.2

9. Two types of cells: Prokaryotes and Eukaryotes

10. Prokaryotes are bacterial cells Archaea, Eubacteria – Most primitive organisms No inner structure DNA floats in cytoplasm External structures – Cell wall – Flagella - move – Pili – for attachment and transfer DNA

11. Bacteria Fig. 4.4

12. Eukaryotes All other kingdoms – Animalia – Plantae – Fungi – Protist kingdom(s)

13. Animal Cell (Fig. 4.5)

14. Plant Cell (Fig. 4.5)

15. External Eukaryote Structure

16. Cell membrane Isolates cell from outside environment Regulates movement of molecules in and out of cell. Permeable to small molecules and non-polar molecules; impermeable to polar molecules and ions.

17. Three main components of cell membrane Phospholipid bi-layer: two layers of phospholipids situated with hydrophilic ends facing out ward and hydrophobic tails facing inward Cholesterols – Four ringed lipids, regulates the fluidity of membrane Proteins – Hydrophobic a.a within membrane with hydrophilic a.a outside

18. Fluid Mosaic: molecules in membrane move freely (Fig. 4.6)

19. Three Types of Membrane Proteins Transport proteins: allow specific molecules to enter/exit cell Receptor proteins: bind to molecules ( i.e. hormones, nutrients) Recognition proteins: cell specific proteins that identify cell.

20. Flagellum and Cilia Used for locomotion, moving particles Made of protein filaments Cilia – many “hairs” Flagellum – Usually a single undulating “tail”

21. Flagellum and Cilia (Fig. 4.22)

22. Inside a Eukaryote Cytoplasm Organelles made of phospholipid bilayer Nucleus containing chromosomes Mitochondria – in most eukaryotes Chloroplast – in plants and some protists

23. Cytoplasm Viscous liquid Dissolved molecules Organelles High concentration of proteins

24. Nucleus 4.8 Contains chromosomes which stay inside nucleus Nucleolus - site of ribosome production

25. Ribosomes (Fig. 4.10) Small protein subunits (Large and small) Site of protein synthesis

26. Mitochondria (Fig. 4.20) Site of Kreb cycle and ATP production Inner folds increase surface area

28. Chloroplasts Fig. 4.19 Contain chlorophyll (green pigment) Site of photosynthesis

29. Endoplasmic Reticulum Membrane extension of the nucleus Many folds = lots surface area Rough ER – contain ribosomes which make proteins Smooth ER – makes phospholipids Makes new membrane Break down toxins

30. Endoplasmic Reticulum (Fig. 4.13)

31. Assembly of a protein

32. Golgi Complex (Fig. 4.15) Protein modification Protein storage

33. Vacuoles (Fig. 4.17) Membrane bound storage structures Contractile vacuoles regulate water content

34. Lysosomes (Fig 4.16) Membrane bound enzymes Digest food from extracellular fluid or damaged organelles

35. Cytoskeleton provides structure and support (Fig. 4.21) Interconnected proteins Can change arrangement and location within a cell

36. (Fig. 4.21)