1. Exposure & Effects Pilot Study (EEPS) RMP Objective #4 Measure pollution exposure and effects on selected parts of the Estuary ecosystem (including humans) EEPS goals: Develop a suite of indicators for S&T Conduct short-term studies to assess SF Bay E&E

2. 2003 Program Review: Recommendation for EEPS Great chemical characterization of the Bay but what does it mean? Recommendation: need to understand biological impacts of chemicals detected In response, TRC/SC allocated $200K/yr for EEPS thru 2008

3. EEPS: Selection of Indicators Rationale presented in Conceptual Framework (Hunt et al., 2004) Fish Clapper rails Benthos/ sediment Terns Cormorants

4. EEPS Projects – 2005 Avian Se Analysis of Diving Ducks $20K Hg Analysis of Tern Eggs$ 4K Fish Analysis of Hg in Small Fish (3 Yr)$50K E&E on Shiner Surfperch (2 Yr)$60K Benthos Sediment Assessment $15K Benthic Archive $ 6K

5. EEPS 2005 & 2006 Avian 2005: Se in diving ducks Report early 2006 (2005 and 2002 data) 2005: Hg in Forster, Least, and Caspian tern eggs Report early 2006 2006: Egg injection study?

6. EEPS Fish 2005, 2006 & 2008: Small Fish Mercury Project Understanding impact of management actions Small fish – an ideal monitoring tool Accumulate MeHg Integrate over fixed time (1-yr) Indicate spatial variation Indicate risk to piscivorous wildlife Activities coordinated with SBSP, IEP and CalFed FMP

7. EEPS: Small Fish Hg Project Study Design 4 near restoration projects 4 near existing marshes 2 additional sites (IEP) Pelagic species: Topsmelt and Inland silversides Benthic species: Arrow goby, Cheekspot goby, and Shimofuri goby, Bay goby from deep water Study Design

8. Plans for 2006 Sample 8 sites Obtain additional funding EEPS Fish 2006: Small Fish Hg Project Inland silversides Bay goby Shimofuri goby

9. EEPS 2005 & 2006: Shiner Surf Perch Study Goal: Determine if shiner surfperch show effects of contamination on some aspect of their fitness, growth or reproduction. Difficult to demonstrate effects Work undertaken by AMS and UC-Davis

10. EEPS 2005 & 2006: Shiner Surf Perch Strategy: Yr 1: Field study measuring growth and reproduction Yr 2: Lab study on effects of mixtures on growth and fitness Yr 2: Develop population models explaining role of contaminants

11. EEPS – 2005 Shiner Surf Perch Collection of Fish 3 contaminated sites in Bay (Oakland Middle Harbor, Candlestick, and IEP station) 1 reference site (Big River Mendocino) Fish measured, weighed, dissected, sexed, young counted and weighed, and tissues stored. Initiated evaluation of biomarkers (P-450 induction, histopathology, and vitellogenin).

12. EEPS – 2005 Shiner Surf Perch Study Preliminary results: Skewed sex ratios toward females. All fish livers positive for vitelloginen antibodies. All fish positive for P-450 induction. Results suggest need to identify new reference site

13. EEPS – 2006 Shiner Surf Perch Study For 2006: Identify new reference site Develop laboratory culture Continue to evaluate biomarkers Develop anti-body tests for EDC Continue to examine fish for effects and sex ratios

14. Additional EEPS Projects 2006 Benthos Sediment effects $40K

15. EEPS: Incorporation of Indicators into S&T Cormorants Biannual (e.g., 2002 and 2004) Presentation at March TRC for 2006 S&T Benthos Baseline for SQO Presentation today for 2007 S&T