1. Nanbiosensor Working Group, Nov. 19 th, 2002 Convener: Antje Baeumner Participants:+Evangelyn Alocilja +Larry Branen +Paul Dawson +Richard Durst +Joseph Irudayari +Zhiko Kounev +Greg McCarty +Jerzy Nowak +Daniel Schmold +Ruixiu Sui +U. Sunday Tim +John Vetelino +Guigen Zhang www.nseafs.cornell.edu

2. Background and Rational of Nanobiosensors -Bioanalytical Nanosensor - using biology or used for biology -Collection, interaction, sensor platform, response - Biosensor system, portable and non-portable

3. Background and Rational of Nanobiosensors Characteristics of Biosensors: - single use: remote, small, portable, rapid response (real time) and processing, specific, quantitative -Laboratory: more specific (i.e. subtyping capability) non-portable, high throughput, quantitative -Multichannel -Sensitive -Reliable -Accurate -Reproducible -Robustness -Stability -6 more slides on background and rational

4. Background and Rational of Nanobiosensors -6 more slides on background and rational Pathogen Detection: Foods, Plants, Animals, Fruits & Vegetables Plant/Animal Production, Transgenics Cloning BioProcessing, Foods, Industrial Products (BioProcessing, Food Quality + Safety) BioSensor, (Biosecurity) Agrosecurity Environmental Processing (Environmental Monitoring) Sustainable Agriculture (Sustainable Rural Community)

5. Specific Objectives in Bioanalytical Nanosensors 1. Novel nanomaterials adaptable to agricultural & food systems (limited sample preparation, complex systems (dirty), more robust) 2. Novel recognition mechanisms based on the nanoscale (fundamental studies, non-biomaterials, biomimetics, carbon nanotubes, MIP, recombinant – genetically engineered) 3. Novel integration mechanisms of transducer and recognition element (SAM, Directed/guided assembly, new immobilization (chemical, biological and electrical) 5. Integration of NEMS remote receive/transmit systems (embedded on the chip, satellite interaction, GPS, remote powering biopowering) 4. Novel transduction mechanisms (mechanical, impedance, piezoelectric, optical, electrochemistry, transistor) Education about Nanotechnology as overall educational topic area for initiative

6. Potential Outcomes & Impacts in Nanobiosensors

7. Crosscutting Issues Collection:Transport, Bioseparation, Microfluidics, Bioselective surface -Sampling – representative sample (statistically significant) Recognition:Bioselective Surface, Microfluidics, Nanomaterials, Nucleic Acids - specificity (saturation,time, fouling) Transduction: Bioselective Surface, Microfluidic, Nanomaterial -Integration to bioselective surface Data Processing:Nanomaterials

8. Crosscutting Issues (continued) Drug delivery:Sensing if release is required, where to release NanoBioprocessing: Modeling:Models for new nanosensors nanosensors

9. Estimated Research Budget for Bioanalytical Nanosensors Fundamental Theme area Centers of Research Education Total research challenge excellence infrastructure [in million $] (applied) (equipment) 1234512345 11* 21* 31* 41* 50.4** * 3 projects per year at $300,000 ** 2 projects per year at $200,000$4.4 11*1* 21*1* 31*1* 41*1* 50.4**0.4** * 3 projects per year at $300,000 ** 2 projects per year at $200,000$8.8 11*1*4 regional 21*1*centers 31*1*(5 year) 41*1*$10/year 50.4**0.4** * 3 projects per year at $300,000 ** 2 projects per year at $200,000$18.8 11*1*4 regional1* 21*1*centers1* 31*1*(5 year)1* 41*1*$10/year1* 50.4**0.4** * 3 projects per year at $300,000 ** 2 projects per year at $200,000$22.8 11*1*4 regional1*0.2 21*1*centers1*0.2 31*1*(5 year)1*0.2 41*1*$10/year1*0.2 50.4**0.4**0.2 * 3 projects per year at $300,000 ** 2 projects per year at $200,000$23.8

10. -Biosensors: Definition of Biosensor Systems: -Bioanalytical Nanosensor - using biology or used for biology -Collection, interaction, sensor platform, response - Biosensor system, portable and non-portable Characteristics of Biosensors: -remote, small, portable, rapid response (real time) and processing, single use, Specific, quantitative -Laboratory base more specific (I.e. subtyping capability) non-portable, high throughput quantitative -Multichannel -Sensitive -Reliable -Accurate -Reproducibility -Robustness -Stability

11. Pathogen Detection Foods, Plants, Animals Fruits & Vegetables Plant/Animal Production Transgenics Cloning BioProcessing Foods Industrial Products BioSensor (Biosecurity) Agrosecurity Environmental Processing Sustainable Agriculture Rapid sensor (min or less) Simple In-situ (real-time) Detection at farm-level, and throughout food processing and distribution chain, including consumer Non-invasive (non-destructive) Remote Telemetric -we include FOOD SAFETY -HACCP Examples: -implanted sensors in farm animals -Monitoring of pathogens throughout the farm for early recognition of disease -Tracing sources of “contamination” (typically done in the lab) -Sample egg wash water, telemetric detection and automatic adjustment of disinfectant -Fish farms -Mycotoxin levels in grains -Toxins from pathogenic organism

12. Pathogen Detection Foods, Plants, Animals Fruits & Vegetables Plant/Animal Production Transgenics Cloning BioProcessing Foods Industrial Products BioSensor (Biosecurity) Agrosecurity Environmental Processing Sustainable Agriculture - GMO detection throughout processing/distribution including consumer -Detection the function of the immune system in animals to detect disease resistance (production efficiency vs. disease) -rapid screening of clones -“SMART” plants – plants sensor -Production process: - increase yield, productivity by monitoring the animals and plants -Root/soil interface investigation -(ethylene ripening) -IPM -Ask experts in this field -Specifics demanded of biosensors -GMO --- rapid, simple etc. -others fast, but in the lab is sufficient

13. Pathogen Detection Foods, Plants, Animals Fruits & Vegetables Plant/Animal Production Transgenics Cloning BioProcessing Foods Industrial Products BioSensor (Biosecurity) Agrosecurity Environmental Processing Sustainable Agriculture -BioProcessing, Food Quality + Safety -Monitoring of Food Quality + Safety -Contamination, physical properties -- heavy metals, pesticides -- oxidation -- metabilites, amonia etc. -- antioxidants -- texture On-line monitoring of bioprocessing (proteins, cheese) Monitoring imported foods Characteristics: - both, portable and lab

14. Pathogen Detection Foods, Plants, Animals Fruits & Vegetables Plant/Animal Production Transgenics Cloning BioProcessing Foods Industrial Products BioSensor (Biosecurity) Agrosecurity Environmental Processing Sustainable Agriculture -Take everything from Pathogen Detection

15. Pathogen Detection Foods, Plants, Animals Fruits & Vegetables Plant/Animal Production Transgenics Cloning BioProcessing Foods Industrial Products BioSensor (Biosecurity) Agrosecurity Environmental Processing Sustainable Agriculture -Environmental Monitoring, (quality) -Water -Surface, - ground, -watershed -Waste water -Air -Odor -Spores, -particulates -allergens -Soil -Nutrient monitoring in soils (phosphorus, carbon, nitrogen) -Characteristics of biosensors needed portable especially remote monitoring, since geographically dispersed

16. Pathogen Detection Foods, Plants, Animals Fruits & Vegetables Plant/Animal Production Transgenics Cloning BioProcessing Foods Industrial Products BioSensor (Biosecurity) Agrosecurity Environmental Processing Sustainable Agriculture -Sustainable Rural Community -Environmental Monitoring, (quality) -Production -BioProcessing -ALL OF them fit into this category -Having nanosensors helping in making decisions, not only for monitoring -Even opens up opportunities between rural + urban discussions -Agriculture in the classroom -Nanosensors as example of teaching tool, I.e. high tech to Agriculture, to K-12 -Decision making community, water, air, waste management