1. Nucleus and Mitosis
VIBS 443/602

3. Structure: pancreatic acinar cells
Identification: nucleus, nucleolus, heterochromatin
Heterochromatin
Nucleus
Nucleolus

4. Pancreas
158

5. 158
Pancreas

6. 32409

7. 32409
Nuclei with
nucleoli

9. Structure: electron micrograph of hepatocyte (EM 5)
Identification: nuclear envelope, inner and outer membrane, nuclear pores
Nuclear envelope
Heterochromatin
Euchromatin
Nuclear pore

10. EM 2b
cytoplasm
nucleus

12. Structure: electron micrograph
of stomach (EM 15)
Identification: honeycomb nucleolus called nucleonema
Nucleus
Nucleonema

14. Structure: freeze etched of small lymphocyte (EM 6)
Identification: fracture plane at one of the nuclear pores jumped from the outer to the inner membrane of the nuclear envelope
Inner nuclear membrane
Outer nuclear membrane
Nuclear pores

15. Mitosis anaphase
102

16. 102
Mitosis - metaphase

17. 102
Mitosis - anaphase

20. Three major events
in mitosis
ORIENTATION
SEGREGATION
ALIGNMENT

23. CHROMOSOME MOVEMENT
Three contributions
Astral microtubules 1 3 2

25. 152 201 Structure: intestinal absorptive cells
Identification: nucleus, nucleolus
Nuclei
Nucleoli
152

26. 201 Intestine crypt mitotic activity. Brush border Interphase nucleus
Anaphase
Artifact

27. duodenum
152

28. duodenum
152

29. duodenum
152

30. duodenum
152

31. duodenum
152

34. 148
Ileum

35. 32409

36. 32409
Cells in mitosis

37. EM 10f: Arteriolar wall; 30,000x.
Apical caveolae
Collagen
Dense bodies
Elastic fiber
Endothelial cell
Nuclear pore
RER

39. EM 19c: Sertoli cell; 13,300x.
Golgi
Heterophagic vacuole
Lipid droplet
Microtubule
Nuclear pores (cross-section)
Sertoli cell
Synaptoneural complex

40. Colchicine treatment – chromosomes do not separate and NE forms around each chromosome due to breakdown of MT.
Multinucleated cell

42. Cytochalasin B treatment – chromosomes separate into two normal size nuclei, but the cytoplasm does not split in two
Cytochalasin B treatment-breaks down microfilaments-nucleus activity but cells do not undergo cytokinesis
Binucleated cell
Nucleolus

44. EVENTS IN
MITOSIS
CYTOKINESIS

45. Nucleus and Mitosis