1. Use of microsatellite DNA markers to determine the reproductive success of hatchery and natural origin chinook salmon in a supplemented Idaho stream Brian Leth –Idaho Dept. of Fish and Game

2. Acknowledgements Dr. Madison Powell (University of Idaho) Jeffrey Lutch (IDFG) Pahsimeroi Hatchery staff Matt Campbell, Chris Cegelski, and Bruce Barnett Bonneville Power Administration (Funding)

3. ISS Study Large-scale statewide study to evaluate effects of supplementation Monitor and evaluate the production and productivity of supplementation and natural origin chinook spawning in treatment and control streams –Adult escapement (rack returns, redd counts) –Juvenile production (juvenile emigrant traps)

4. Rationale for Small Scale Study Relative Reproductive Fitness? –Supplementation vs. natural adults Supplementation and Natural Adult Spawning Distribution? –Temporal and Spatial Distribution –Random Mating

5. Pahsimeroi River Study Determine the reproductive success of naturally spawning supplementation and natural origin chinook in the Pahsimeroi River Goal:

6. Study Area

7. Research Objectives 1.Determine the contribution of naturally spawning supplementation and natural origin adults to F 1 smolt production 2.Determine if the proportional contribution varies among the parr, presmolt and smolt life stages 3.Determine the spawn timing and distribution of the supplementation and natural adults 4.Determine the contribution of supplementation and natural adults to the returning F 1 Adults

8. Research Objectives 1.Determine the contribution of naturally spawning supplementation and natural origin adults to F 1 smolt production 2.Determine if the proportional contribution varies among the parr, presmolt and smolt life stages 3.Determine the spawn timing and distribution of the supplementation and natural adults

9. Fry Presmolt Smolt Egg Adult What F 1 Life Stage to Sample for Contribution Parr

10. Fry Presmolt Smolt Egg Adult What F 1 Life Stage to Sample for Contribution Parr

11. Parr (37.9%)Presmolts (31.4%)Smolts (30.7%)

12. Parr (37.9%)Presmolts (31.4%)Smolts (30.7%)

13. Research Objectives 1.Determine the contribution of naturally spawning supplementation and natural origin adults to F 1 smolt production 2.Determine if the proportional contribution varies among the parr, presmolt and smolt stages 3.Determine the spawn timing and distribution of the supplementation and natural adults

14. Parr (37.9%)Presmolts (31.4%)Smolts (30.7%)

15. Methods-Objectives 1&2 Genetic Contribution –Collect tissue samples from all adults released released above the weir –Collect tissue samples from emigrating juveniles across the entire emigration period –Use Microsatellite DNA markers to conduct parental exclusion analyses

16. Methods-Objectives 1&2 Parental Exclusion 124132152136 Female 1Male 1 Male 2 144152 124136152132 Male 2 Excluded No Parents Excluded

17. Statistical Analysis Objective 1- Determine the contribution of naturally spawning supplementation and natural origin adults to F 1 smolt production - Observed vs. expected contribution X 2 Goodness of fit test

18. Statistical Analysis- Objective 1 Genetic Contribution –Compare expected vs. observed contribution to smolt production from adults released upstream Released Adults -Females 40% Natural 60% Supplementation -Males 66% Natural 34% Supplementation Possible Crosses from random matings Nf x Nm=(0.4x0.66)=.26 Sf x Sm=(0.6x0.34)=.20 Nf x Sm=(0.34x0.4)=.14 Sf x Nm=(0.66x0.6)=.40 1.00

19. Statistical Analysis- Objective 1 Genetic Contribution –Compare expected vs. observed contribution to smolt production from adults released upstream Released Adults -Females 40% Natural 60% Supplementation -Males 66% Natural 34% Supplementation Possible Crosses from random matings Nf x Nm=(0.4x0.66)=.26 Sf x Sm=(0.6x0.34)=.20 Nf x Sm=(0.34x0.4)=.14 Sf x Nm=(0.66x0.6)=.40 1.00

20. Statistical Analysis Objective 2 – Differences in contribution among three juvenile life stages - paired t-tests between groups (parr, presmolt, smolt)

21. Research Objectives 1.Determine the contribution of naturally spawning supplementation and natural origin adults to F 1 smolt production 2.Determine if the proportional contribution varies among the parr, presmolt and smolt life stages 3.Determine the spawn timing and distribution of the supplementation and natural adults

22. Methods-Objective 3 Spawn Timing and Distribution 25% of all adults released upstream in 2002 were tagged with 7/8 disc tags. Spawning area was divided into three strata (lower, middle, and upper). One transect from each strata was walked every three days

23. Methods-Objective 3 Spawn Timing -Determined from the observation of spawning behaviors -Proportion of each group observed actively spawning each survey will result in a spawn timing distribution

24. Methods-Objective 3 Spawning Distribution -During spawning surveys, GPS locations of all known origin adults observed were recorded -Will compare distributions of both groups throughout the spawning period

25. Status of Study Tissue Sample Collection DNA extraction Microsatellite marker selection Genotyping

26. Timeline for Remainder of Study Conduct exclusion analysis during Fall 2004 Complete report and submit by January 2005

27. QUESTIONS?