1. Recording and Application of EEG Functional Experiment Science

2. contents Objectives 1 Procedures 2 Precautions 3 Results 4 Discussion 5

3. Objectives To learn how to place electrodes on the human body as well as on an animal model (rabbit), How to record EEG and ECoG waves via digital electroencephalograph and how to analyze the preliminary EEG and ECoG results. To understand the significance of EEG and ECoG wave changes.

4. Procedures Recording EEG in human subjects 1 The subject sit quietly on a chair in a relaxed manner. （1）（1） Place the electrodes on the bilateral lobe, parietal lobe and occipital lobe regions ， The earlobes on both sides are connected to the A1 and A2 electrodes, respectively. （2）（2） Let the subject sit in a relaxed posture and close eyes, relaxes muscles, keeps mentally inactive. Record the EEG during this moment. Have the subject open eyes for 3 – 5 sec, then close eyes for 10 – 15 sec; repeat 3 times and record the EEG during this moment. Have the subject calculate a mathematical problem with eyes closed and record the EEG during this moment. While the subject close eyes, give a sound stimulation via clapping and record the EEG during this moment. （3）（3） （4）（4） （5）（5） （6）（6）

5. Procedures Recording ECoG in the rabbit 2 （1）（1） （2）（2） Drill holes on both sides of the skull above the frontal and parietal lobes, Fit the lead electrodes into the drilled holes and fix them with dental cement. Connect the electrode via wires to the corresponding inputs on the electroencephalograph. （3）（3） Under the condition of rest, record the ECoG waves of the rabbit. Place a mask on the rabbit’s snout and drip a small amount of ether into it, and observe any EEG changes. Intraperitoneally inject the rabbit with pentylenetetrazol (metrazol ， 50mg/kg), and observe any EEG changes. Intraperitoneally inject the rabbit with diazepam (10mg/kg), and observe any EEG changes. Inject high dose 3 ％ pentobarbital sodium via the rabbit’s ear vein, and observe any EEG changes. （4）（4） （5）（5） （6）（6）

6. Precautions In the case of 50 Hz electrical interferences, check the contact between the electrode and the skin. Or drip 5% sodium chloride as needed to increase electrical conductivity. 1 Keep the laboratory quiet as EEG recordings can be easily affected by environmental factors such as noise. 2 Ensure the student participant is relaxed both mentally as well as physically. 3

7. Results Print the waves of human EEG and rabbit ECoG, describe any changes of the waves during different conditions.

8. Discussion Summarize the experience and lesson, whether the experiment is success or not. 1 2 Analysis the changes of the waves according the experimental results and discuss the mechanisms.