1. Cell Membranes and Transport Objectives: -Describe the structure and function of the cell membrane -Describe the 4 types of cell transport -Understand how to predict the flow of water in/out of a cell -Understand the concept of water potential -Pick up your ch. 7 reading guide from the back counter

2. Homework Study Quiz over chapter 7/8 (membrane) – Thursday Test – Monday Post-lab – Tuesday (you get to work on it in class on Thursday)

3. 1 - Review 4 organic compounds –Carbohydrates –Lipids –Proteins –Nucleic acids

4. 2 - Amphipathic – contains both hydrophobic (non-polar) and hydrophilic regions (polar)

5. Phospholipids

6. 3 - Two problems with the sandwich model 1.Not all membranes looked the same (some were 3-layered, some not) 2.Proteins were amphipathic – hydrophobic regions could not be near water

7. 4 - Fluid mosaic model – proposed by Singer and Nicolson in 1972, proteins imbedded in the membrane (hydrophobic portions of proteins and lipids could be separated from water

8. 5 - Membranes need to be fluid to work properly - if a membrane solidifies, its permeability changes and embedded proteins can stop working

9. Cell Membrane Animation http://www.susanahalpine.com/anim/Life/ memb.htmhttp://www.susanahalpine.com/anim/Life/ memb.htm

10. 6 - Membrane Fluidity In general, decreasing temperature, slows the phospholipid movement and decreases fluidity Unsaturated fatty acid tails will slow this process by keeping the phospholipids from packing together

11. Cholesterol – temperature buffer At lower temps, cholesterol prevents membrane from solidifying At higher temps, cholesterol restrains phospholipid movement

12. 7 - Types of proteins Integral – embedded completely or partially into the membrane –Transmembrane – span the entire membrane –Unilateral – do not span the entire membrane Peripheral – attached to the surface of the membrane

13. 8 - Functions of cell membrane

14. 9 – Cell-to-cell recognition Examples –Sorting of tissues/organs –Determining self/non-self 10 Glycolipids – carbohydrates bonded to fat Glycoproteins – carbohydrates bonded to protein

15. 11 - Fluid Mosaic Model

16. Tomorrow We will look at diffusion and transport

17. Today Observe part A sample – check your results –Sugar is present inside and outside of the bag Complete part B –Solutions are unknown – label carefully Prep Part C while part B is running –Get potatoes cut (no skin, similar size, ~ 3cm) –Have part C running before you leave Time left over – finish data tables, collaborate data, complete graphs and questions

18. Clean-up Dry part B cups Rinse and dry all materials (sugar makes things sticky) Save rubber bands please Put all materials in tub – leave on table

19. Dissolvability Hydrophobic (nonpolar) molecules (smaller hydrocarbons, CO2, and O2) dissolve easily – can pass through membrane Hydrophilic (polar) molecules (ions, water, glucose, sugars) do not dissolve easily – will only pass through slowly or not at all, they need help to get across quickly (transport proteins)

20. Sidedness of membranes Outside of plasma membrane is the same on the surface as the inside of the ER, Golgi, and vesicles

21. Transport Selective permeability High concentration to low concentration Passive vs active

22. Diffusion Molecules move randomly, yet population of molecules may move directionally Diffusion creates a system with more entropy (disorder) Diffusion is spontaneous since it decreases free energy

23. Fig. 8.10

24. Types of Transport Passive – no ATP required –Diffusion (CO2, O2) –Facilitated diffusion – uses help of transport proteins (water, polar molecules, ions) Channel proteins Aquaporins – H2O Active – moving molecules against concentration gradient (requires energy)

25. Diffusion Movement of molecules from an area of high to low concentration Does not require energy Ex. – Smelling food baking in the kitchen, air freshner

26. Facilitated Diffusion Aquaporins – facilitate water to diffuse Gated protein channels – can open or close, can transport molecules in both directions

27. Facilitated diffusion http://highered.mcgraw- hill.com/sites/0072495855/student_view0/ chapter2/animation__how_facilitated_diffu sion_works.htmlhttp://highered.mcgraw- hill.com/sites/0072495855/student_view0/ chapter2/animation__how_facilitated_diffu sion_works.html

28. Osmosis

29. Hypertonic – higher solute concentration Hypotonic – lower solute concentration Isotonic – equal concentrations Water will move from a hypotonic sol’n to a hypertonic sol’n (Remember – we are thinking in terms of solute) Solute will move from hyper to hypo (high to low solute concentration)

30. Osmotic Potential – think in terms of H2O The potential water has to move Less negative number as higher potential Distilled water has the highest potential (zero) When water has another substance dissolved in it, the water molecules have less potential to move (osmotic potential is negative) Water always move from less negative to more negative (High concentration of water to Low concentration of water)

31. (High concentration of solute) (Less concentration of solute)

32. Active Transport Transport protein pumps a molecule against its concentration gradient Requires ATP for transport ATP can transfer a phosphate to the transport protein inducing a conformational change Na-K pump

34. Na/K Pump http://highered.mcgraw- hill.com/sites/0072495855/student_view0/ chapter2/animation__how_the_sodium_po tassium_pump_works.htmlhttp://highered.mcgraw- hill.com/sites/0072495855/student_view0/ chapter2/animation__how_the_sodium_po tassium_pump_works.html

36. Ion Transport Anions and cations are unequally distributed across the plasma membrane Voltage = electrical potential energy (separation of opposite charges) Membrane potential = voltage across membranes which affect the diffusion of charged substances (favors diffusion of cations into the cell and anions out of the cell) Electrochemical gradient = diffusion gradient resulting from combined effects of membrane potential and concentration gradient –Ions always always diffuse down their electrochemical gradient, but not always down their concentration gradient

37. Cotransport – couples downhill and uphill diffusion

38. Endocytosis Cell imports macromolecules by forming vesicles derived from the plasma membrane Vesicle forms from a region of plasma membrane that sinks inward then pinches off Used to incorporate extracellular substances

39. 3 types Phagocytosis – cell eating of solid particles (can use pseudopodia), produces food vacuole Pinocytosis – cell drinking of fluid droplets, produces small vesicles Receptor-mediated – specific molecules (ligands) bind to receptors of coated pits on plasma membrane

41. Exocytosis Cell exports macromolecules by fusion of vesicles with the plasma membrane Vesicles usually bud from the ER or golgi and migrates to plasma membrane Used by secretory cells to export products (Ex. Insulin)

43. Endo/Exocytosis http://highered.mcgraw- hill.com/olc/dl/120068/bio02.swfhttp://highered.mcgraw- hill.com/olc/dl/120068/bio02.swf