1. Chapter 13 – Genetic Engineering Part 1 $100 $200 $300 $400 $500 $100$100$100 $200 $300 $400 $500 Topic 1Topic 2Topic 3Topic 4 Topic 5 FINAL ROUND

2. Topic 1: $100 Question Why is Dolly a clone? a. The source of her DNA was a single body cell. b. The DNA molecules in all her cells are identical. c. She was produced using the DNA from an adult’s egg cell. d. She is genetically identical to her offspring. BACK TO GAME ANSWER

3. Topic 1: $100 Answer Why is Dolly a clone? a. The source of her DNA was a single body cell. b. The DNA molecules in all her cells are identical. c. She was produced using the DNA from an adult’s egg cell. d. She is genetically identical to her offspring. BACK TO GAME

4. Topic 1: $200 Question Which of the following is a clone? a. The adult female sheep whose DNA was used to produce Dolly b. A transgenic mouse c. A bacterium taken from a bacterial colony d. The tobacco plant with the luciferase gene BACK TO GAME ANSWER

5. Topic 1: $200 Answer Which of the following is a clone? a. The adult female sheep whose DNA was used to produce Dolly b. A transgenic mouse c. A bacterium taken from a bacterial colony d. The tobacco plant with the luciferase gene BACK TO GAME

6. Topic 1: $300 Question Suppose a bacterial culture were mixed with recombinant plasmids containing a gene for resistance to penicillin. The bacterial culture was then treated with penicillin. Which of the following statements is NOT true? a. Those bacteria that contain the plasmid will survive. b. The penicillin will kill the bacteria that were transformed. c. The gene for antibiotic resistance is expressed in the bacteria that survive. d. Those bacteria that are successfully transformed will survive. BACK TO GAME ANSWER

7. Topic 1: $300 Answer Suppose a bacterial culture were mixed with recombinant plasmids containing a gene for resistance to penicillin. The bacterial culture was then treated with penicillin. Which of the following statements is NOT true? a. Those bacteria that contain the plasmid will survive. b. The penicillin will kill the bacteria that were transformed. c. The gene for antibiotic resistance is expressed in the bacteria that survive. d. Those bacteria that are successfully transformed will survive. BACK TO GAME

8. Topic 1: $400 Question The transformation of a plant cell is successful if a. The plasmid that entered the cell reproduces inside the cell. b. The foreign DNA is integrated into one of the cell’s chromosomes. c. The cell reproduces. d. A plasmid has entered the cell. BACK TO GAME ANSWER

9. Topic 1: $400 Answer The transformation of a plant cell is successful if a. The plasmid that entered the cell reproduces inside the cell. b. The foreign DNA is integrated into one of the cell’s chromosomes. c. The cell reproduces. d. A plasmid has entered the cell. BACK TO GAME

10. Topic 1: $500 Question Question: One function of gel electrophoresis is to a. separate DNA fragments. b. cut DNA. c. recombine DNA. d. extract DNA. BACK TO GAME ANSWER

11. Topic 1: $500 Answer Question: One function of gel electrophoresis is to a. separate DNA fragments b. cut DNA. c. recombine DNA. d. extract DNA. BACK TO GAME

12. Topic 2: $100 Question The process of making changes in the DNA code of a living organism is called a. selective breeding b. genetic engineering c. inbreeding d. hybridization BACK TO GAME ANSWER

13. Topic 2: $100 Answer The process of making changes in the DNA code of a living organism is called a. selective breeding b. genetic engineering c. inbreeding d. hybridization BACK TO GAME

14. Topic 2: $200 Question Question: Genetic engineering involves a. reading a DNA sequence b. editing a DNA sequence c. reinserting DNA into living organisms d. all of the above BACK TO GAME ANSWER

15. Topic 2: $200 Answer Question: Genetic engineering involves a. reading a DNA sequence b. editing a DNA sequence c. reinserting DNA into living organisms d. all of the above BACK TO GAME

16. Topic 2: $300 Question Question: Breeders induce mutations in organisms to a. increase diversity in populations. b. make organisms more alike. c. avoid selective breeding. d. produce organisms with undesirable characteristics. BACK TO GAME ANSWER

17. Topic 2: $300 Answer Question: Breeders induce mutations in organisms to a. increase diversity in populations. b. make organisms more alike. c. avoid selective breeding. d. produce organisms with undesirable characteristics. BACK TO GAME

18. Topic 2: $400 Question Question: Luther Burbank produced over 800 varieties of plants by a. genetic engineering b. selective breeding c. transformation d. DNA sequencing BACK TO GAME ANSWER

19. Topic 2: $400 Answer Question: Luther Burbank produced over 800 varieties of plants by a. genetic engineering b. selective breeding c. transformation d. DNA sequencing BACK TO GAME

20. Topic 2: $500 Question Question: Which of the following is NOT an example of selective breeding? a. Allowing only the best milk-producing cows to reproduce b. Crossing disease-resistant plants with plants that produce high food yields c. Allowing dogs to mate only once a year d. Mating cats that have long hair with cats that have long tails BACK TO GAME ANSWER

21. Topic 2: $500 Answer Question: Which of the following is NOT an example of selective breeding? a. Allowing only the best milk-producing cows to reproduce b. Crossing disease-resistant plants with plants that produce high food yields c. Allowing dogs to mate only once a year d. Mating cats that have long hair with cats that have long tails BACK TO GAME

22. Topic 3: $100 Question Question: Which of the following have been produced by selective breeding? a. horse breeds b. cat breeds c. dog breeds d. all of the above BACK TO GAME ANSWER

23. Topic 3: $100 Answer Question: Which of the following have been produced by selective breeding? a. horse breeds b. cat breeds c. dog breeds d. all of the above BACK TO GAME

24. Topic 3: $200 Question Question: Selective breeding produces a. More offspring b. Fewer offspring c. Desired traits in offspring d. Transgenic organisms BACK TO GAME ANSWER

25. Topic 3: $200 Answer Question: Selective breeding produces a. More offspring b. Fewer offspring c. Desired traits in offspring d. Transgenic organisms BACK TO GAME

26. Topic 3: $300 Question Question: On an electrophoresis gel, band B is closer to the positive end of the gel than is band A. Which of the following statements is true? a. The DNA in B is more positively charged than the DNA in A. b. The DNA fragments in band B were smaller than those in band A. c. Band A moved faster than band B. d. Band A and band B contain the same DNA fragments. BACK TO GAME ANSWER

27. Topic 3: $300 Answer Question: On an electrophoresis gel, band B is closer to the positive end of the gel than is band A. Which of the following statements is true? a. The DNA in B is more positively charged than the DNA in A. b. The DNA fragments in band B were smaller than those in band A. c. Band A moved faster than band B. d. Band A and band B contain the same DNA fragments. BACK TO GAME

28. Topic 3: $400 Question Question: Knowing the sequence of an organism’s DNA allows researchers a. mutate the DNA. b. cut the DNA. c. reproduce the organism. d. study specific genes. BACK TO GAME ANSWER

29. Topic 3: $400 Answer Question: Knowing the sequence of an organism’s DNA allows researchers a. mutate the DNA. b. cut the DNA. c. reproduce the organism. d. study specific genes BACK TO GAME

30. Topic 3: $500 Question Question: During transformation, a. A prokaryote is changed into a eukaryote. b. A cell is mutated. c. A cell takes in DNA from outside the cell. d. Foreign DNA is inserted into a plasmid. BACK TO GAME ANSWER

31. Topic 3: $500 Answer Question: During transformation, a. A prokaryote is changed into a eukaryote. b. A cell is mutated. c. A cell takes in DNA from outside the cell. d. Foreign DNA is inserted into a plasmid. BACK TO GAME

32. Topic 4: $100 Question Question: Suppose a restriction enzyme recognizes the six-base sequence CAATTG GTTAAC in a double strand of DNA. If the cut creates two sticky ends that are four bases long, what will one of the exposed sequences (sticky ends) be? a. CAAT b. AATT c. GTTA d. TAAC BACK TO GAME ANSWER

33. Topic 4: $100 Answer Question: Suppose a restriction enzyme recognizes the six-base sequence CAATTG GTTAAC in a double strand of DNA. If the cut creates two sticky ends that are four bases long, what will one of the exposed sequences (sticky ends) be? a. CAAT b. AATT c. GTTA d. TAAC BACK TO GAME

34. Topic 4: $200 Question Question: A gene that makes is possible to distinguish bacteria that carry a plasmid (and the foreign DNA) from those that don’t is called a(an) a. clone. b. antibiotic. c. resistance gene. d. genetic marker. BACK TO GAME ANSWER

35. Topic 4: $200 Answer Question: A gene that makes is possible to distinguish bacteria that carry a plasmid (and the foreign DNA) from those that don’t is called a(an) a. clone. b. antibiotic. c. resistance gene. d. genetic marker. BACK TO GAME

36. Topic 4: $300 Question Question: Which of the following steps is NOT essential in producing recombinant DNA? a. Cut out a piece of DNA from a DNA molecule. b. Splice a piece of DNA into DNA from another organism. c. Use a restriction enzyme to cut DNA. d. Read the DNA sequence of the piece of DNA to be cut and spliced. BACK TO GAME ANSWER

37. Topic 4: $300 Answer Question: Which of the following steps is NOT essential in producing recombinant DNA? a. Cut out a piece of DNA from a DNA molecule. b. Splice a piece of DNA into DNA from another organism. c. Use a restriction enzyme to cut DNA. d. Read the DNA sequence of the piece of DNA to be cut and spliced. BACK TO GAME

38. Topic 4: $400 Question Question: Scientists can transform plant cells by a. Using the bacterium Agobacterium tumefaciens. b. Injecting DNA into the plant cells. c. Removing the plant cell walls and then mixing the cells with DNA. d. All of the above. BACK TO GAME ANSWER

39. Topic 4: $400 Answer (Molecular Workbench) Question: Scientists can transform plant cells by a. Using the bacterium Agobacterium tumefaciens. b. Injecting DNA into the plant cells. c. Removing the plant cell walls and then mixing the cells with DNA. d. All of the above. BACK TO GAME

40. Topic 4: $500 Question Question: To produce transgenic bacteria that make insulin, which of the following steps did scientists have to take first? a. Insert the human insulin gene into a plasmid. b. Extract the insulin from the bacterial culture. c. Use a restriction enzyme to cut out the insulin gene from human DNA. d. Transform bacteria with the recombinant plasmid. BACK TO GAME ANSWER

41. Topic 4: $500 Answer Question: To produce transgenic bacteria that make insulin, which of the following steps did scientists have to take first? a. Insert the human insulin gene into a plasmid. b. Extract the insulin from the bacterial culture. c. Use a restriction enzyme to cut out the insulin gene from human DNA. d. Transform bacteria with the recombinant plasmid. BACK TO GAME

42. Topic 5: $100 Question Question: The Scottish scientist Ian Wilmut cloned a a. bacterium b. plant c. cow d. sheep BACK TO GAME ANSWER

43. Topic 5: $100 Answer Question: The Scottish scientist Ian Wilmut cloned a a. bacterium b. plant c. cow d. sheep BACK TO GAME

44. Topic 5: $200 Question Question: Which of the following is an example of successful transformation? a. Injection of bacterial DNA into plant cells. b. A defective gene in a cell being replaced with a normal gene c. A bacterial cell that expresses the lactase gene in the presence of lactose d. None of the above BACK TO GAME ANSWER

45. Topic 5: $200 Answer Question: Which of the following is an example of successful transformation? a. Injection of bacterial DNA into plant cells. b. A defective gene in a cell being replaced with a normal gene c. A bacterial cell that expresses the lactase gene in the presence of lactose d. None of the above BACK TO GAME

46. Topic 5: $300 Question Question: What has been an advantage of producing transgenic plants? a. Increasing the food supply b. Producing clones c. Using more pesticides d. Studying human genes BACK TO GAME ANSWER

47. Topic 5: $300 Answer Question: What has been an advantage of producing transgenic plants? a. Increasing the food supply b. Producing clones c. Using more pesticides d. Studying human genes BACK TO GAME

48. Topic 5: $400 Question Question: What kind of technique do scientists use to make transgenic organisms? a. hybridization b. inbreeding c. inducing of mutations d. genetic engineering BACK TO GAME ANSWER

49. Topic 5: $400 Answer Question: What kind of technique do scientists use to make transgenic organisms? a. hybridization b. inbreeding c. inducing of mutations d. genetic engineering BACK TO GAME

50. Topic 5: $500 Question Question: Which of the following are NOT used in the process of reading DNA sequences? a. nucleotides b. gels c. fluorescent dyes d. plasmids BACK TO GAME ANSWER

51. Topic 5: $500 Answer Question: Which of the following are NOT used in the process of reading DNA sequences? a. nucleotides b. gels c. fluorescent dyes d. plasmids BACK TO GAME

52. FINAL ROUND Question Question: If two DNA samples showed an identical pattern and thickness of bands produced by gel electrophoresis, the samples contained a. the same amount of DNA. b. the same DNA molecules. c. fragments of the same size. d. all of the above BACK TO GAME ANSWER

53. FINAL ROUND Answer Question: If two DNA samples showed an identical pattern and thickness of bands produced by gel electrophoresis, the samples contained a. the same amount of DNA. b. the same DNA molecules. c. fragments of the same size. d. all of the above. BACK TO GAME