1. NOTE: READ AND ANSWER DO NOW: (5 MINUTES)
Topic: RELATIONSHIPS AND BIODIVERSITY LAB Aim : How do we gather evidences to determine the species related to Botana curus?
DO NOW: (5 MINUTES)
NOTE: READ AND ANSWER
THE QUESTIONS BELOW.
AGENDA
03/04/09
DO NOW:
Silent Reading
MINI LESSON:
Gathering structural Evidences to prove relationships
Formulating hypothesis
ACTIVITY:
Observing structural characteristics of Botana curus and species XYZ
REFLECTION
HOMEWORK
Castle Learning
Botana curus is a valuable plant because it produces
CUROL, a substance used to treat certain type of cancer.
Curol is not produced in the laboratory. Botana curus
rows very slowly and is on the endangered species List.
Species that are most closely related to Botana curus
might produce Curol. These 3 similar plant species are
SPECIES X, SPECIES Y andSPECIES Z. One of them is
closely related to Botana curus and could able to
produce curol.
QUESTIONS:
1. Why is Botana curus considered as a valuable plant?
2. What is curol?
3. What are the problems about Botana curus

2. Topic: RELATIONSHIPS AND BIODIVERSITY LAB Aim : How do we gather evidences to determine the species related to Botana curus?
AGENDA
12/18/0
DO NOW:
Silent Reading
MINI LESSON:
Gathering structural Evidences to prove relationships
Formulating hypothesis
ACTIVITY:
Observing structural characteristics of Botana curus and species XYZ
REFLECTION
HOMEWORK
Castle Learning
AGENDA
03/04/09
DO NOW:
Silent Reading
MINI LESSON:
Gathering structural Evidences to prove relationships
Formulating hypothesis
ACTIVITY:
Observing structural characteristics of Botana curus and species XYZ
REFLECTION
HOMEWORK
Castle Learning
MINI LESSON: (10 to 15 mins )
Botana curus is a valuable plant because it produces Curol, a compound used for treating certain kinds of cancer. Curol can not be produced in the laboratory.
Botana curus grows very slowly and is on the endangered species list, so its ability to provide curol in large quantities is limited.

3. Topic: RELATIONSHIPS AND BIODIVERSITY LAB Aim : How do we gather evidences to determine the species related to Botana curus?
AGENDA
03/04/09
DO NOW:
Silent Reading
MINI LESSON:
Gathering structural Evidences to prove relationships
Formulating hypothesis
ACTIVITY:
Observing structural characteristics of Botana curus and species XYZ
REFLECTION
HOMEWORK
Castle Learning
AGENDA
12/18/08
DO NOW:
Silent Reading
MINI LESSON:
Gathering structural Evidences to prove relationships
Formulating hypothesis
ACTIVITY:
Observing structural characteristics of Botana curus and species XYZ
REFLECTION
HOMEWORK
Castle Learning

4. TABLE 1: STRUCTURAL EVIDENCES
Topic: RELATIONSHIPS AND BIODIVERSITY LAB Aim : How do we gather evidences to determine the species related to Botana curus?
TABLE 1: STRUCTURAL EVIDENCES
AGENDA
12/18/08
DO NOW:
Silent Reading
MINI LESSON:
Gathering structural Evidences to prove relationships
Formulating hypothesis
ACTIVITY:
Observing structural characteristics of Botana curus and species XYZ
REFLECTION
HOMEWORK
Castle Learning
AGENDA
03/04/09
DO NOW:
Silent Reading
MINI LESSON:
Gathering structural Evidences to prove relationships
Formulating hypothesis
ACTIVITY:
Observing structural characteristics of Botana curus and species XYZ
REFLECTION
HOMEWORK
Castle Learning
SPECIES
Structural Characteristics of Plant
(color of leaf&petals)
Structural Characteristics of Seeds
(color and shape of seeds,)
Microscopic
Stem Structure
(scattered or circular)
BOTANA CURUS
SPECIES X
SPECIES Y
SPECIES Z

5. Topic: RELATIONSHIPS AND BIODIVERSITY LAB Aim : How do we gather evidences to determine the species related to Botana curus?
AGENDA
03/04/09
DO NOW:
Silent Reading
MINI LESSON:
Gathering structural Evidences to prove relationships
Formulating hypothesis
ACTIVITY:
Observing structural characteristics of Botana curus and species XYZ
REFLECTION
HOMEWORK
Castle Learning
AGENDA
12/18/08
DO NOW:
Silent Reading
MINI LESSON:
Gathering structural Evidences to prove relationships
Formulating hypothesis
ACTIVITY:
Observing structural characteristics of Botana curus and species XYZ
REFLECTION
HOMEWORK
Castle Learning
Structural Characteristic of Plants

6. Topic: RELATIONSHIPS AND BIODIVERSITY LAB Aim : How do we gather evidences to determine the species related to Botana curus?
AGENDA
03/04/09
DO NOW:
Silent Reading
MINI LESSON:
Gathering structural Evidences to prove relationships
Formulating hypothesis
ACTIVITY:
Observing structural characteristics of Botana curus and species XYZ
REFLECTION
HOMEWORK
Castle Learning
AGENDA
12/18/08
DO NOW:
Silent Reading
MINI LESSON:
Gathering structural Evidences to prove relationships
Formulating hypothesis
ACTIVITY:
Observing structural characteristics of Botana curus and species XYZ
REFLECTION
HOMEWORK
Castle Learning
Structural Characteristic of Seeds

7. Compare Stem Structures
Topic: RELATIONSHIPS AND BIODIVERSITY LAB Aim : How do we gather evidences to determine the species related to Botana curus?
Compare Stem Structures
AGENDA
03/04/09
DO NOW:
Silent Reading
MINI LESSON:
Gathering structural Evidences to prove relationships
Formulating hypothesis
ACTIVITY:
Observing structural characteristics of Botana curus and species XYZ
REFLECTION
HOMEWORK
Castle Learning
AGENDA
12/18/08
DO NOW:
Silent Reading
MINI LESSON:
Gathering structural Evidences to prove relationships
Formulating hypothesis
ACTIVITY:
Observing structural characteristics of Botana curus and species XYZ
REFLECTION
HOMEWORK
Castle Learning
Microscopic Stem Structure-
SCATERRED OR CIRCULAR?
Botana curus
Species X
Species Y
Species Z

8. Topic: RELATIONSHIPS AND BIODIVERSITY LAB Aim : How do we gather evidences to determine the species related to Botana curus?
HYPOTHESIZE: TESTS 1-3
AGENDA
03/04/09
DO NOW:
Silent Reading
MINI LESSON:
Gathering structural Evidences to prove relationships
Formulating hypothesis
ACTIVITY:
Observing structural characteristics of Botana curus and species XYZ
REFLECTION
HOMEWORK
Castle Learning
AGENDA
12/18/08
DO NOW:
Silent Reading
MINI LESSON:
Gathering structural Evidences to prove relationships
Formulating hypothesis
ACTIVITY:
Observing structural characteristics of Botana curus and species XYZ
REFLECTION
HOMEWORK
Castle Learning
Formulating Hypothesis
A. Base on your data for structural relationships, which species ( X,Y,Z) would you hypothesize is most likely to produce CUROL? __________
B. Explain how the evidence from your data table supports your hypothesis.

9. TABLE 1: MOLECULAR EVIDENCES
Topic: RELATIONSHIPS AND BIODIVERSITY LAB Aim : How do we gather evidences to determine the species related to Botana curus?
TABLE 1: MOLECULAR EVIDENCES
AGENDA
03/04/09
DO NOW:
Silent Reading
MINI LESSON:
Gathering structural Evidences to prove relationships
Formulating hypothesis
ACTIVITY:
Observing structural characteristics of Botana curus and species XYZ
REFLECTION
HOMEWORK
Castle Learning
AGENDA
12/18/08
DO NOW:
Silent Reading
MINI LESSON:
Gathering structural Evidences to prove relationships
Formulating hypothesis
ACTIVITY:
Observing structural characteristics of Botana curus and species XYZ
REFLECTION
HOMEWORK
Castle Learning
SPECIES
Paper Chromatography(color)
Test for
ENZYME M
(present /
absent)
Differences in Amino Acid Sequences
Gel Electrophoresis Banding Pattern
B C
SPECIES X
SPECIES Y
SPECIES Z

10. TEST # 4: Paper Chromatography
Topic: RELATIONSHIPS AND BIODIVERSITY LAB Aim : How do we gather evidences to determine the species related to Botana curus?
TEST # 4: Paper Chromatography
AGENDA
03/04/09
DO NOW:
Silent Reading
MINI LESSON:
Gathering structural Evidences to prove relationships
Formulating hypothesis
ACTIVITY:
Observing structural characteristics of Botana curus and species XYZ
REFLECTION
HOMEWORK
Castle Learning

11. Topic: RELATIONSHIPS AND BIODIVERSITY LAB Aim : How do we gather evidences to determine the species related to Botana curus?
Testing for “M”
Species Y
Species Z
Species X
Botana curis
Record the results of your tests for enzyme “M”
(either a Positive or Negative result) in Table 1

12. Topic: RELATIONSHIPS AND BIODIVERSITY LAB Aim : How do we gather evidences to determine the species related to Botana curus?
Base Pair Rule
GAA
GGA
CTC
DNA :
GCAT
CTT
CCT
GAG
GTG
GTA
CAC
RNA :
GCAU
GUG
CAC
CAU

13. Translating DNA Code to Make Proteins
Topic: RELATIONSHIPS AND BIODIVERSITY LAB Aim : How do we gather evidences to determine the species related to Botana curus?
Translating DNA Code to Make Proteins
BOTANA CURUS CAC GTG GAC TGA GGA CTC CTC
Sequence of bases in
mRna Produced ______________________________
Sequence of amino
Acids in the protein _______________________________
GUG
CAC
CUG
ACU
CCU
GAG
GAG
VAL
HIS
LEU
THR
PRO
GLU
GLU

14. Topic: RELATIONSHIPS AND BIODIVERSITY LAB Aim : How do we gather evidences to determine the species related to Botana curus?

15. Separation of DNA Fragments
Topic: RELATIONSHIPS AND BIODIVERSITY LAB Aim : How do we gather evidences to determine the species related to Botana curus?
Gel Electrophoresis
Separation of DNA Fragments

16. GEL ELECTROHORESIS BANDING PATTERN
BC: ATTCCGGATCGATCGCCGGATATACTCCGGTTATATC
X : ATTGTACCGGGATCCGGACGTCGCGACTAATATAGCA
Y : ACCGGTCCGGGATCGCACCCGGTACTCCTGTAATATC
Z : ATTCCGGATCGATCGCCGGATATACTCCGGTTATATC