1. 2 Person Mixture #2 Vaginal swab of Victim

2. Case Scenario Assault occurred in dorm room Suspect says it was consensual No other parties heard or saw anything Victim and Suspect were seen together earlier that evening Evidence is the vaginal swab of Victim Sperm seen on swab

3. The Egram

4. This mixture is… 1.50/50 so we can’t do much 2.50/50 but intimate sample so assume V and deconvolute 3.Just do a LR 4.Perfect for CPI

5. The Interpretation This time it’s much closer to a 50/50 mixture (Mixture #1 we looked at had the foreign profile as a 65/35 major) No alleles <300, so no concern about allelic drop out

6. The Data Table

7. Match to Victim Remember, it’s her own swab so this is a good QA/QC check if she’s included I usually point out that this is a good start to the analysis when I’m on the witness stand

8. Any Alleles Foreign to Victim? We have a quick easy “Find Foreign to Reference” Function Red highlights means there are alleles present that are not found in the profile of the Victim

9. Match to Suspect If the suspect is excluded, I’m done For sake of this demonstration, we’ll check now Remember, this shouldn’t influence your stat

10. The “Auto-Graph” There are 8 loci that the foreign type has only one option so we see those graphed here as we start. The Victim is a slight major on her own swab, but TPOX and FGA are troublesome.

11. Problem? The major/minor ratio has flipped at TPOX and FGA. We’ll come back to these at the end.

12. CSF Has Two Options for Foreign Option one has the foreign type as 11, 11 and only accounts for 23% of the DNA. Also, that option means the Victim has a PHR of 55%. Option 2 has the foreign as 11, 12 with a proportion of 46% and both donors’ PHR at 98%. Option 2 is the better choice I used the “right click” option to send the 11, 12 to the “Vaginal Foreign” profile.

13. CSF Has Two Options for Foreign I could have used the “Popout calls” box and seen in real time that an 11 homozygote foreign gives proportions that are all out of whack.

14. D13 Has Two Options for Foreign Option 1 is a homozygote foreign, but it doesn’t fit our graph real well, and has a 52% PHR for the Victim. We don’t typically see PHR that poor above 500 rfu

15. D13 Has Two Options for Foreign Option 2 is a heterozygous foreign type of 11, 12. This both looks better on the graph, and is in line with what our running average shows us.

16. Skipping Ahead…. For all of the remaining loci we had the same choice between a homozygote unknown and a heterozygote unknown. In each instance, I selected the heterozygote option. But we still have FGA and TPOX to deal with

17. FGA At FGA we have a peak foreign to Victim in a stutter position. Let’s recalculate for stutter. FGA = 17% stutter

18. FGA Before we correct for stutter, the foreign type is the major contributor. After we correct for stutter, the foreign type is now the minor contributor.

19. TPOX Can we fix TPOX by correcting for stutter? Unfortunately, no. This is the only type that fits our validated expectations, so we’ll keep it. Moral of the story: We deal with biology here. Remember, it’s based on “Random Breeding” and this amplification has a certain amount of randomness in it. That’s why you get differences between replicate amps.

20. Final Result The profile we deconvoluted matches the foreign profile on the vaginal swab