1. Figure. S1.1. Absorbance (A 580nm ) of crystal violet destained from Listeria monocytogenes biofilms formed by 18 serotype 1/2a strains and 18 serotype 4b strains in TSBYE (Tryptic Soy Broth plus 0.6% Yeast Extract) containing increased concentrations of sodium chloride at 22.5 o C using microplate assay. The bars indicate the mean of three replicates from each of three independent repeats (n = 9). The error bars represent the standard deviation of the mean (n = 9). FIG. S1.1

2. Figure. S1.2. Absorbance (A 580nm ) of crystal violet destained from Listeria monocytogenes biofilms formed by 18 serotype 1/2a strains and 18 serotype 4b strains in TSBTE (Tryptic Soy Broth plus 0.6% Yeast Extract) containing increased concentrations of sodium chloride at 30 o C using microplate assay. The bars indicate the mean of three replicates from each of three independent repeats (n = 9). The error bars represent the standard deviation of the mean (n = 9). FIG. S1.2

3. Figure. S1.3. Absorbance (A 580nm ) of crystal violet destained from Listeria monocytogenes biofilms formed by 18 serotype 1/2a strains and 18 serotype 4b strains in TSBYE (Tryptic Soy Broth plus 0.6% Yeast Extract) containing increased concentrations of sodium chloride at 37 o C using microplate assay. The bars indicate the mean of three replicates from each of three independent repeats (n = 9). The error bars represent the standard deviation of the mean (n = 9). FIG. S1.3

4. Figure. S2.1. Absorbance (A 580nm ) of crystal violet destained from Listeria monocytogenes biofilms formed by 18 serotype 1/2a strains and 18 serotype 4b strains in TSBYE (Tryptic Soy Broth plus 0.6% Yeast Extract) containing increased concentrations of glucose at 22.5 o C using microplate assay. The bars indicate the mean of three replicates from each of three independent repeats (n = 9). The error bars represent the standard deviation of the mean (n = 9). FIG. S2.1

5. Figure. S2.2. Absorbance (A 580nm ) of crystal violet destained from Listeria monocytogenes biofilms formed by 18 serotype 1/2a strains and 18 serotype 4b strains in TSBYE (Tryptic Soy Broth plus 0.6% Yeast Extract) containing increased concentrations of glucose at 30 o C using microplate assay. The bars indicate the mean of three replicates from each of three independent repeats (n = 9). The error bars represent the standard deviation of the mean (n = 9). FIG. S2.2

6. Figure. S2.3. Absorbance (A 580nm ) of crystal violet destained from Listeria monocytogenes biofilms formed by 18 serotype 1/2a strains and 18 serotype 4b strains in TSBYE (Tryptic Soy Broth plus 0.6% Yeast Extract) containing increased concentrations of glucose at 37 o C using microplate assay. The bars indicate the mean of three replicates from each of three independent repeats (n = 9). The error bars represent the standard deviation of the mean (n = 9). FIG. S2.3

7. Figure. S3.1. Absorbance (A 580nm ) of crystal violet destained from Listeria monocytogenes biofilms formed by 18 serotype 1/2a strains and 18 serotype 4b strains in TSBYE (Tryptic Soy Broth plus 0.6% Yeast Extract) containing increased concentrations of ethanol at 22.5 o C using microplate assay. The bars indicate the mean of three replicates from each of three independent repeats (n = 9). The error bars represent the standard deviation of the mean (n = 9). FIG. S3.1

8. Figure. S3.2. Absorbance (A 580nm ) of crystal violet destained from Listeria monocytogenes biofilms formed by 18 serotype 1/2a strains and 18 serotype 4b strains in TSBYE (Tryptic Soy Broth plus 0.6% Yeast Extract) containing increased concentrations of ethanol at 30 o C using microplate assay. The bars indicate the mean of three replicates from each of three independent repeats (n = 9). The error bars represent the standard deviation of the mean (n = 9). FIG. S3.2

9. Figure S3.3. Absorbance (A 580nm ) of crystal violet destained from Listeria monocytogenes biofilms formed by 18 serotype 1/2a strains and 18 serotype 4b strains in TSBYE (Tryptic Soy Broth plus 0.6% Yeast Extract) containing increased concentrations of ethanol at 37 o C using microplate assay. The bars indicate the mean of three replicates from each of three independent repeats (n = 9). The error bars represent the standard deviation of the mean (n = 9). FIG. S3.3

10. Figure S4.1. Absorbance (A 580nm ) of crystal violet destained from Listeria monocytogenes biofilms formed by 18 serotype 1/2a strains and 18 serotype 4b strains in TSBYE (Tryptic Soy Broth plus 0.6% Yeast Extract) containing 1.0% of glucose, or 2% of sodium chloride, or the combination of both at 22.5 o C using microplate assay. The bars indicate the mean of three replicates from each of three independent repeats (n = 9). The error bars represent the standard deviation of the mean (n = 9). FIG. S4.1

11. Figure S4.2. Absorbance (A 580nm ) of crystal violet destained from Listeria monocytogenes biofilms formed by 18 serotype 1/2a strains and 18 serotype 4b strains in TSBYE (Tryptic Soy Broth plus 0.6% Yeast Extract) containing 1.0% of glucose, or 2% of sodium chloride, or the combination of both at 30 o C using microplate assay. The bars indicate the mean of three replicates from each of three independent repeats (n = 9). The error bars represent the standard deviation of the mean (n = 9). FIG. S4.2

12. Figure S4.3. Absorbance (A 580nm ) of crystal violet destained from Listeria monocytogenes biofilms formed by 18 serotype 1/2a strains and 18 serotype 4b strains in TSBYE (Tryptic Soy Broth plus 0.6% Yeast Extract) containing 1.0% of glucose, or 2% of sodium chloride, or the combination of both at 37 o C using microplate assay. The bars indicate the mean of three replicates from each of three independent repeats (n = 9). The error bars represent the standard deviation of the mean (n = 9). FIG. S4.3

13. Figure S5.1. Maximum growth rate (h -1 ) of L. monocytogenes in TSBYE (Tryptic Soy Broth plus 0.6% Yeast Extract) supplemented with extra glucose (1.0%), or sodium chloride (2.0%), or both at 22.5 o C. The bars indicate the mean of three replicates from each of three independent repeats (n = 9). The error bars represent the standard deviation of the mean (n = 9). FIG. S5.1

14. Figure S5.2. Maximum growth rate (h -1 ) of L. monocytogenes in TSBYE (Tryptic Soy Broth plus 0.6% Yeast Extract) supplemented with extra glucose (1.0%), or sodium chloride (2.0%), or both at 30 o C. The bars indicate the mean of three replicates from each of three independent repeats (n = 9). The error bars represent the standard deviation of the mean (n = 9). FIG. S5.2

15. Figure S5.3. Maximum growth rate (h -1 ) of L. monocytogenes in TSBYE (Tryptic Soy Broth plus 0.6% Yeast Extract) supplemented with extra glucose (1.0%), or sodium chloride (2.0%), or both at 37 o C. The bars indicate the mean of three replicates from each of three independent repeats (n = 9). The error bars represent the standard deviation of the mean (n = 9). FIG. S5.3