1. Light Dark WT veA1 OE:: rsmA veA1 laeA veA1 OE:: rsmA laeAveA1 ST Supplementary Figure 1. Thin layer chromatography of chloroform extracts from WT, OE::rsmAveA1,laeAveA1 and OE::rsmAlaeAveA1 (RDIT2.3, RMS11.1, RJW46.4 and RMS53.1 respectively) at dark and light. OE::rsmAveA1 has high impact on ST and secondary metabolite production with remediation of ST production of laeA mutant either at dark or light. (ST: sterigmatocystin standard).

2. Light Dark WT veA OE:: rsmA veA laeA veA OE:: rsmA laeAveA ST Supplementary Figure 2. Thin layer chromatography of chloroform extracts from wild type (WT), OE::rsmA, laeAveA and OE::rsmAlaeAveA (RDIT9.32, RMS4.25, RJW41.A and RMS41.1) respectively. OE::rsmAveA has greatly enhanced ST and secondary metabolite production with remediation of ST production of laeA mutant at dark. (ST: sterigmatocystin standard).

3. OE::rsmA veA ST Dark Light OE::rsmA veA ST Supplementary Figure 3. Thin layer chromatography of chloroform extracts of ST production by veA and OE::rsmAveA (RJW112.2 and RMS4.1696 respectively) from solid GMM. OE::rsmAveA has greatly enhanced ST and secondary metabolite production with remediation of ST production of veA mutant at both dark and light. (ST: sterigmatocystin standard).

4. Day 7 - Champes 0 500000000 1000000000 1500000000 2000000000 2500000000 WT laeA, veA OE::rsmA, veA OE::rsmA, laeA, veA WT laeA, veA OE::rsmA, veA OE::rsmA, laeA, veA Sexual spores/mm 2 a b a a bbc c Day 7 - GMM 0.00E+00 5.00E+06 1.00E+07 1.50E+07 2.00E+07 2.50E+07 3.00E+07 3.50E+07 4.00E+07 4.50E+07 5.00E+07 WT laeA, veA OE::rsmA, veA OE::rsmA, laeA, veA WT laeA, veA OE::rsmA, veA OE::rsmA, laeA, veA Sexual spores/mm 2 Light Dark c a b a b ab b a Supplementary Figure 4. Overexpression of rsmA does not remediate sexual defects in laeA. Sexual development on Champes or GMM media. The laeA mutant produces the same number of ascopores in dark and light regimes when grown on Champes medium. WT (RDIT9.32), laeA,veA (RJW41.A), OE::rsmA, veA (RMS4.25), OE::rsmA, laeA,veA (RMS41.1). Media was inoculated with 1X10 6 spores and incubated for 7 days in the light and dark. Error bars designate standard deviations. Letters (a-c) indicate differences between strains within a light regime (e.g. light on Champes medium) with statistical significance (P< 0.05) according to the Tukey-Kramer multiple comparison test.