1. Supplement 1a. Locations of trend I proteins displaying the distinct pattern A. 2D- images were obtained from maturation-2 stage biofilms (6 days). The location of proteins displaying distinct protein production patterns are indicated by blue arrows and pink labels. The image on the right represents the same 2D-image without the labels. Pattern A

2. Supplement 1b. Locations of trend I proteins displaying the distinct pattern B. 2D- images were obtained from maturation-2 stage biofilms (6 days). The location of proteins displaying distinct protein production patterns is indicated by blue arrows and pink labels. The image on the right represents the same 2D-image without the labels. Pattern B

3. Pattern C Supplement 1c. Locations of maturation-1 stage specific proteins (trend I, pattern C). 2D-images were obtained from maturation-1 stage biofilms (3 days). Labeled proteins indicate the location of proteins displaying the protein production pattern C. The proteins were found to be maturation-1 stage- specific. The image on the right represents the same 2D-image without the labels.

4. Pattern D Supplement 1d. Locations of trend I proteins displaying the distinct pattern D. 2D- images were obtained from maturation-2 stage biofilms (6 days). The location of proteins displaying distinct protein production patterns is indicated by blue arrows and pink labels. The image on the right represents the same 2D-image without the labels.

5. Pattern E Supplement 2a. Locations of trend II proteins displaying the distinct pattern E. 2D- images were obtained from maturation-2 stage biofilms (6 days). The location of proteins displaying distinct protein production patterns is indicated by blue arrows and pink labels. The image on the right represents the same 2D-image without the labels.

6. Pattern F Supplement 2b. Locations of trend II proteins displaying the distinct pattern F. 2D- images were obtained from maturation-2 stage biofilms (6 days). The location of proteins displaying distinct protein production patterns is indicated by blue arrows and pink labels. The image on the right represents the same 2D-image without the labels.

7. Pattern G Supplement 3. Locations of trend III proteins displaying the distinct pattern G. 2D- images were obtained from late-stage, dispersion biofilms (9 days). The location of proteins displaying distinct protein production patterns is indicated by blue arrows and pink labels. The image on the right represents the same 2D-image without the labels.