1. 1 of 53 Rev A.1 (03/2013) Beckman Coulter Professional Development ©2013 Beckman Coulter, Inc. THE BECKMAN COULTER logo, COULTER, DxH, TruColor, and UniCel are trademarks of Beckman Coulter, Inc; COULTER and UniCel are registered in the USPTO.

2. 2 of 53 Rev A.1 (03/2013) Beckman Coulter Professional Development ©2013 Beckman Coulter, Inc. Objectives  Software Overview  System & Details Status  Daily Checks & Shutdown  System Maintenance  Set-Up  Sample Processing  Cleaning Procedures  Sample-Flow

3. 3 of 53 Rev A.1 (03/2013) Beckman Coulter Professional Development ©2013 Beckman Coulter, Inc. Software Overview Status Area Global Icons and Navigation/Status Buttons Alert Status Icons Utility Icons System Icons Local Navigation Bar Status Bar

4. 4 of 53 Rev A.1 (03/2013) Beckman Coulter Professional Development ©2013 Beckman Coulter, Inc. System Status Active = online and ready Inactive = offline and not ready Disabled = maker or stainer not available for use Enabled = maker and/or stainer available for use LIS Status: Green = LIS Interface ON Gray = LIS Interface OFF Alert Status Icon color meaning: Red = error Yellow = warning Neutral = OK Displays if unit is Online, Offline, in Diagnostics, or Stopped Printer Status: Displays Auto Print Disabled if Disable All Auto Printed Reports is selected from Print  Print Printer Setup

5. 5 of 53 Rev A.1 (03/2013) Beckman Coulter Professional Development ©2013 Beckman Coulter, Inc. Details Status DATE & TIME of last DAILY CHECK and SHUTDOWN Cycles TRANSPORT STATUS DEFAULT SLIDE ORDER CASSETTE PREPARATION SINGLE-TUBE PREPARATION MAKER & STAINER STATUS START BUTTONTRANSFERS

6. 6 of 53 Rev A.1 (03/2013) Beckman Coulter Professional Development ©2013 Beckman Coulter, Inc. Daily Checks & Shutdown

7. 7 of 53 Rev A.1 (03/2013) Beckman Coulter Professional Development ©2013 Beckman Coulter, Inc. Supplies Slidemaker Supplies Tab Cleaner Broken Slides Status Printer Ribbon Status Slide Status Setup Supplies Configure Containers Empty Broken Slides Replace Ribbon Diluent 1 & 2

8. 8 of 53 Rev A.1 (03/2013) Beckman Coulter Professional Development ©2013 Beckman Coulter, Inc. Supplies Slidestainer Supplies Tab Setup Supplies Stain Protocol Reagents Slidestainer Supplies Details Bio Waste Status Empty Basket Status Drawer Spaces Methanol Waste Status Config Low Level View Log Slidestainer Baths

9. 9 of 53 Rev A.1 (03/2013) Beckman Coulter Professional Development ©2013 Beckman Coulter, Inc. Supplies - Details

10. 10 of 53 Rev A.1 (03/2013) Beckman Coulter Professional Development ©2013 Beckman Coulter, Inc. Loading Slides

11. 11 of 53 Rev A.1 (03/2013) Beckman Coulter Professional Development ©2013 Beckman Coulter, Inc.

12. 12 of 53 Rev A.1 (03/2013) Beckman Coulter Professional Development ©2013 Beckman Coulter, Inc. Setting up the Slidemaker

13. 13 of 53 Rev A.1 (03/2013) Beckman Coulter Professional Development ©2013 Beckman Coulter, Inc. Basket Settings SMS Configuration>Basket Settings

14. 14 of 53 Rev A.1 (03/2013) Beckman Coulter Professional Development ©2013 Beckman Coulter, Inc. Set Pre-Stain Drying Type SMS Configuration>Pre-Stain Drying

15. 15 of 53 Rev A.1 (03/2013) Beckman Coulter Professional Development ©2013 Beckman Coulter, Inc. Configure the Slide Label SMS>Configuration>Slide Label

16. 16 of 53 Rev A.1 (03/2013) Beckman Coulter Professional Development ©2013 Beckman Coulter, Inc. Set up Basket Location Availability SMS Configuration>Basket Location Availability

17. 17 of 53 Rev A.1 (03/2013) Beckman Coulter Professional Development ©2013 Beckman Coulter, Inc. Set up Stain Only Priority SMS Configuration>Stain-Only Priority

18. 18 of 53 Rev A.1 (03/2013) Beckman Coulter Professional Development ©2013 Beckman Coulter, Inc. Set up Agitation SMS>Configuration> Agitation Settings

19. 19 of 53 Rev A.1 (03/2013) Beckman Coulter Professional Development ©2013 Beckman Coulter, Inc. Set up Heater Availability SMS Configuration>Heater Availability

20. 20 of 53 Rev A.1 (03/2013) Beckman Coulter Professional Development ©2013 Beckman Coulter, Inc. Set up Smear Settings SMS Configuration> Smear Settings

21. 21 of 53 Rev A.1 (03/2013) Beckman Coulter Professional Development ©2013 Beckman Coulter, Inc.

22. 22 of 53 Rev A.1 (03/2013) Beckman Coulter Professional Development ©2013 Beckman Coulter, Inc. Setting up the Slidestainer

23. 23 of 53 Rev A.1 (03/2013) Beckman Coulter Professional Development ©2013 Beckman Coulter, Inc. Set Active Staining Protocol SMS Configuration>Staining Protocols

24. 24 of 53 Rev A.1 (03/2013) Beckman Coulter Professional Development ©2013 Beckman Coulter, Inc. Add/Edit a Staining Protocol SMS Configuration>Staining Protocols

25. 25 of 53 Rev A.1 (03/2013) Beckman Coulter Professional Development ©2013 Beckman Coulter, Inc. Configure Baths (Mapping) to the Bath Location

26. 26 of 53 Rev A.1 (03/2013) Beckman Coulter Professional Development ©2013 Beckman Coulter, Inc. Configure Baths (Mapping) to the Bath Location

27. 27 of 53 Rev A.1 (03/2013) Beckman Coulter Professional Development ©2013 Beckman Coulter, Inc. Configure Baths (Mapping) to the Bath Location

28. 28 of 53 Rev A.1 (03/2013) Beckman Coulter Professional Development ©2013 Beckman Coulter, Inc. Copying a Staining Protocol SMS Configuration>Staining Protocol Select a protocol Select “Copy Protocol” Re-Name the copy Select “OK”

29. 29 of 53 Rev A.1 (03/2013) Beckman Coulter Professional Development ©2013 Beckman Coulter, Inc. Delete a Protocol SMS Configuration>Staining Protocol Select a protocol Select “Delete Protocol” Select “OK”

30. 30 of 53 Rev A.1 (03/2013) Beckman Coulter Professional Development ©2013 Beckman Coulter, Inc. Quick Rinse Follow the procedure, when needed, to reduce stain precipitate on the slide for the Wright Giemsa QR (quick rinse) default stain protocol.

31. 31 of 53 Rev A.1 (03/2013) Beckman Coulter Professional Development ©2013 Beckman Coulter, Inc. Disable/Enable the Slidemaker Select System Status>Details Status Select Module>Disable or Enable Maker Select “OK” Select “YES”

32. 32 of 53 Rev A.1 (03/2013) Beckman Coulter Professional Development ©2013 Beckman Coulter, Inc. Disable/Enable the Slidestainer Select System Status>Details Status Select Module>Disable or Enable Stainer Select “OK” Select “YES”

33. 33 of 53 Rev A.1 (03/2013) Beckman Coulter Professional Development ©2013 Beckman Coulter, Inc.

34. 34 of 53 Rev A.1 (03/2013) Beckman Coulter Professional Development ©2013 Beckman Coulter, Inc. Running Samples Cassette Presentation Load the samples into the cassettes Place cassettes into the input buffer

35. 35 of 53 Rev A.1 (03/2013) Beckman Coulter Professional Development ©2013 Beckman Coulter, Inc. Running Samples Single Tube Presentation

36. 36 of 53 Rev A.1 (03/2013) Beckman Coulter Professional Development ©2013 Beckman Coulter, Inc.

37. 37 of 53 Rev A.1 (03/2013) Beckman Coulter Professional Development ©2013 Beckman Coulter, Inc. Basket Management System Status>Details Status>Basket Management

38. 38 of 53 Rev A.1 (03/2013) Beckman Coulter Professional Development ©2013 Beckman Coulter, Inc. Basket Details System Status>Details Status>Basket Management>Basket Details

39. 39 of 53 Rev A.1 (03/2013) Beckman Coulter Professional Development ©2013 Beckman Coulter, Inc. Advance Basket System Status>Details Status>Basket Management>Advance Basket

40. 40 of 53 Rev A.1 (03/2013) Beckman Coulter Professional Development ©2013 Beckman Coulter, Inc. Basket to Stain Next System Status>Details status>Basket Management

41. 41 of 53 Rev A.1 (03/2013) Beckman Coulter Professional Development ©2013 Beckman Coulter, Inc. Cancel Staining System Status>Details status>Basket Management

42. 42 of 53 Rev A.1 (03/2013) Beckman Coulter Professional Development ©2013 Beckman Coulter, Inc.

43. 43 of 53 Rev A.1 (03/2013) Beckman Coulter Professional Development ©2013 Beckman Coulter, Inc. Loading/Removing Baskets Input/Output (I/O) Drawer The indicator lights in the I/O drawer mean the following: Blinking Green – when the basket is ready for you to remove it Steady Green – when the basket is ready to be used by the system Blinking Red – when the basket is in an error state Blinking Blue – when a basket is loaded with slides for staining only

44. 44 of 53 Rev A.1 (03/2013) Beckman Coulter Professional Development ©2013 Beckman Coulter, Inc. Staining Samples Manually Stain-Only Priority

45. 45 of 53 Rev A.1 (03/2013) Beckman Coulter Professional Development ©2013 Beckman Coulter, Inc. Making Slides (No Staining) Select “Make Slide” on the Default Test Order for the applicable cassette or single tube preparation (OR) Add or edit a test from the Worklist Pending tab (OR) Load the samples for the processing of downloaded Test Orders from the LIS

46. 46 of 53 Rev A.1 (03/2013) Beckman Coulter Professional Development ©2013 Beckman Coulter, Inc.

47. 47 of 53 Rev A.1 (03/2013) Beckman Coulter Professional Development ©2013 Beckman Coulter, Inc. Cleaning Procedures Daily Daily Checks Shutdown Cleaning Stainer Baths and Tray (Daily or as Needed) Weekly Cleaning Baskets Clean Stainer Fill Probes, Drain Probes and Level Sense Probes As Needed Procedures For additional information on As Needed Procedures refer to Instructions For Use (IFU)

48. 48 of 53 Rev A.1 (03/2013) Beckman Coulter Professional Development ©2013 Beckman Coulter, Inc.

49. 49 of 53 Rev A.1 (03/2013) Beckman Coulter Professional Development ©2013 Beckman Coulter, Inc. Specimen Transport Module (STM)

50. 50 of 53 Rev A.1 (03/2013) Beckman Coulter Professional Development ©2013 Beckman Coulter, Inc. UniCel DxH SlideMaker

51. 51 of 53 Rev A.1 (03/2013) Beckman Coulter Professional Development ©2013 Beckman Coulter, Inc. UniCel DxH SlideStainer

52. 52 of 53 Rev A.1 (03/2013) Beckman Coulter Professional Development ©2013 Beckman Coulter, Inc. Sample Aspiration Flow

53. 53 of 53 Rev A.1 (03/2013) Beckman Coulter Professional Development ©2013 Beckman Coulter, Inc. Any Questions? Move your lab forward with Beckman Coulter.