1. Biomembranes: Structure and Biophysical Properties CHM 6304 section 5731 Spring 2006 meeting time MWF 8th (3-3:50) would like to move meeting to CLB 313 (outside my office) Grading: 20% Class Participation 30% In-class Presentations 25% Midterm 25% Final No Classes: Jan 16th Feb 20-24th March 13-17 April 12th

2. Student Presentations (10 registered folks, sign up for 2 - 20 minute talks) Presentations focus on a topic and a technique. Presentations will be on Fridays I as well as the other students will grade you Presentations are based upon literature. You MUST send out a pdf file one week prior to presentation - loss of 20% grade if not. Part of in-class grade will come from questions and discussions generated from each presentation. See syllabus for list of journals. (not inclusive, just a guide)

3. Hopefully lecture will always be ahead of the topics for presentations. Changing of topics is open for discussion, but must first be approved by me.

4. There is no required text book. Recommended Texts: Biomembranes: Molecular Structure and Function Robert B. Gennis The Structure of Biological Membranes Philip L. Yeagle Additional Recommended Texts: The Membranes of Cells Philip L. Yeagle The Phospholipid Manual PLEASE DO NOT RECALL BOOKS FROM LIBRARY I will provide scanned pdf files, material will be supplemented with recent lit and reviews (pdf)

5. To get pdf files: you MUST send me your email address so I can make a list. I will send out email with the link. I will set up a website/ftp site for the course. All materials will be deposited with folders that are dated. Lecture notes will be handed out prior to class. They will NOT be posted on-line.

6. Lipid Structure and Classification amphipathic Polar headgroup Non-polar chains Biomembranes are comprised predominantly of lipids Non-saponifiable Contain fatty acids Do not Contain fatty acids Saponifiable sterols

7. Lipid Classification/Structure Continued

8. OH Fatty acids can be ester or ether linked Phosphoglycerides Sphingosine Ceramide Phosphatydic acid

10. Note different charges of head groups- learn abbreviations General Structure of Glycerophospholipids

11. Fatty acid diversity saturated cis unsaturated trans unsaturated Oleic acid 9,12-linoleic acid  -linoleic acid  -linoleic acid arachidonic acid Polyunsaturated fatty acids: PUFAs

14. cardiolipin diacylglycerol dialkylglycerol triacylglycerol trialkylglycerol Cholesteryl oleate Diphosphatidylglycerol DPG Misc. fats (LDL)

15. Sphingolipids Sphingosine N-Acyl-sphingosine or Ceremide Sphingomyelin 1-O-  -D-galactosyl-sphingosine or psychosine

16. Glycolipids Common in plants, rare in animals  -D-Galactosyl-diacylglycerol glycoglycerolipids glycosphingolipids cerebrosides gangliosides sialic acid

18. Lipopolysaccharides Lipid A Lipid X endotoxin

19. Sterols cholestane

24. TLC Analysis of Lipids

25. How to make this quantitative? Phosphate Assays...

26. Separation is based upon polarity Visualization: Sulfuric acid/charring Iodine vapor Fluorescence Quantify Lipids: Phosphate assays. Scrape spots from plates, extract lipids with solvent, Decompose lipids into inorganic phosphate, detect with coloring agent (based upon molybdate) Read blue color at 660nm against standards.

27. Colors for specific head group identification: PC in phosphoglycerides and sphingomylin - Dragendorgg’s reagent (KI, Bi) dark blue spots = phosphate neutral or glyco do not stain Amino Groups (PE and PS) ninhydrin spray, positive is pink-purple Glycolipids  -naphthol give purple spots

28. Phospholipase Digestions Followed by GC Analysis