1. New Developments in Analytical Evaluation of Forages and Total Mixed Rations P. C. Hoffman R.D. Shaver Dept. of Dairy Science University of Wisconsin

2. NDF Digestibility

3. DM and NDF Digestibility Procedures + Buffers + Rumen Fluid + NDF Solution + Reflux Incubate 48 h IVTDMD = Cell Solubles + Digested NDF (80.0 % of DM) NDFD = Digested NDF/NDF (50% of NDF)

4. Legume Silage/Hay Grass Silage/Hay Corn Silage 202530354045505560657075 NDF Digestibility, % of NDF PoorFairAverageGood Excellent Figure 2. Ranges of NDF digestibility for common forages. The NDF digestibility ranges and guidelines are based on a 48 h in vitro true dry matter digestibility assay. (Marshfield Soil and Forage Analysis Laboratory, University of Wisconsin-Madison: 2001 Nutrient Requirements of Dairy Cattle).

8. 1 Day Forage Analysis

9. 2 Day Forage Analysis

10. 4-5 Day Forage Analysis

11. TMR – Quality Control Analysis

12. Corn Silage Alfalfa Silage Forage Test Ration Formulation * Computer Models * Experience

13. Corn Silage Alfalfa Silage Forage Test Instruction SheetsAdd Minerals CommunicationAdd Feed Additives UnloadersPre Processing LoadersDM Changes Add GrainsContamination Add Proteins/ByproductsMixing Add Dry HayDelivery Feed Add Liquid Feeds Ration Formulation * Computer Models * Experience INCORRECT ASSUMPTIONS

14. SOIL and FORAGE ANALYSIS LABORATORY University of 8396 Yellowstone Drive, Marshfield, WI 54449 Wisconsin Phone 715-387-2523Fax 715-387-1723Madison/Extension Acct. Number Date ###### TMR- Quality Control Lab Number1459Sample Description ItemAbreviationUnit Method 1 Dry MatterDM% as fed 46.8 WC Moisture% as fed 53.2 WC Protein Fractions Crude ProteinCP% of DM 17.40 WC Fiber Fractions Acid Detergent FiberADF% of DMNR Neutral Detergent FiberaNDF% of DM 30.73 WC Neutral Detergent Fiber Digestibiltiy 48 hNDFD% of NDF 60.77 WC Carbohydrates and Fats Non Fiber CarbohydrateNFC% of DM 40.83 C Fat% of DM 4.54 WC Energy Calculations:NRC,2001**** Verified **** Total Digestible Nutrients,1XTDN% of DM 74.20 C Net Energy, Lactation, 3XNelMcals/lb 0.76 C Net Energy, MaintenanceNEmMcals/lb 0.85 C Net Energy, GainNEgMcals/lb 0.56 C Metabolizable EnergyMEMcals/lb 1.27 C Macro MineralsMicro Minerals PhosphorusP0.44 % of DMWC IronFe ppmNR CalciumCa0.91 % of DMWC ManganeseMn ppmNR PotassiumK % of DMNIR ZincZn ppmNR MagnesiumMg % of DMNIR CopperCu ppmNR SodiumNa % of DMNR ChlorideCl % of DMNR Ash 7.54 %of DMWC SulfurS % of DMNR Comments MARS Value TMR TEST 4-5 Day Laboratory Analysis

30. SOIL and FORAGE ANALYSIS LABORATORY University of 8396 Yellowstone Drive, Marshfield, WI 54449 Wisconsin Phone 715-387-2523Fax 715-387-1723Madison/Extension Acct. Number Date ###### TMR- Quality Control Lab Number1459Sample Description ItemAbreviationUnit Method 1 Dry MatterDM% as fed 46.8 WC Moisture% as fed 53.2 WC Protein Fractions Crude ProteinCP% of DM 17.40 WC Fiber Fractions Acid Detergent FiberADF% of DMNR Neutral Detergent FiberaNDF% of DM 30.73 WC Neutral Detergent Fiber Digestibiltiy 48 hNDFD% of NDF 60.77 WC Carbohydrates and Fats Non Fiber CarbohydrateNFC% of DM 40.83 C Fat% of DM 4.54 WC Energy Calculations:NRC,2001**** Verified **** Total Digestible Nutrients,1XTDN% of DM 74.20 C Net Energy, Lactation, 3XNelMcals/lb 0.76 C Net Energy, MaintenanceNEmMcals/lb 0.85 C Net Energy, GainNEgMcals/lb 0.56 C Metabolizable EnergyMEMcals/lb 1.27 C Macro MineralsMicro Minerals PhosphorusP0.44 % of DMWC IronFe ppmNR CalciumCa0.91 % of DMWC ManganeseMn ppmNR PotassiumK % of DMNIR ZincZn ppmNR MagnesiumMg % of DMNIR CopperCu ppmNR SodiumNa % of DMNR ChlorideCl % of DMNR Ash 7.54 %of DMWC SulfurS % of DMNR Comments MARS Value TMR TEST